[1]丁振源,何冬成,祭仲石,等.不同养殖模式下斑点叉尾鱼回池塘水质及微生物群落特征[J].江苏农业学报,2026,42(07):1429-1440.[doi:doi:10.3969/j.issn.1000-4440.2026.07.014]
　DING Zhenyuan,HE Dongcheng,JI Zhongshi,et al.Water quality and microbial community characteristics in channel catfish (Ictalurus punctatus) ponds under different aquaculture patterns[J].,2026,42(07):1429-1440.[doi:doi:10.3969/j.issn.1000-4440.2026.07.014]
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不同养殖模式下斑点叉尾鱼回池塘水质及微生物群落特征()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1429-1440
栏目:
畜牧兽医·水产养殖
出版日期:
2026-07-31

文章信息/Info

Title:
Water quality and microbial community characteristics in channel catfish (Ictalurus punctatus) ponds under different aquaculture patterns
作者:
丁振源1; 何冬成2; 祭仲石3; 韦艳3; 管卫兵1
(1.上海海洋大学海洋科学与生态环境学院,上海201306；2.无锡三智生物科技有限公司,江苏无锡214145；3.上海市海丰水产养殖有限公司,江苏盐城224151)
Author(s):
DING Zhenyuan1; HE Dongcheng2; JI Zhongshi3; WEI Yan3; GUAN Weibing1
(1.College of Oceanography and Ecological Science, Shanghai Ocean University, Shanghai 201306, China;2.Wuxi Sanzhi Biotechnology Co., Ltd., Wuxi 214145, China;3.Shanghai Haifeng Aquaculture Co., Ltd., Yancheng 224151, China)
关键词:
斑点叉尾鱼回; 混养模式; 水质指标; 微生物群落
Keywords:
Ictalurus punctatus; polyculture system; water quality indicators; microbial community
分类号:
S964.3
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.014
文献标志码:
A
摘要:
为探究斑点叉尾鱼回(Ictalurus punctatus)混养模式的生态可持续性并优化生态养殖体系,本研究通过国家标准水质检测法和16S rRNA基因高通量测序技术,分析比较斑点叉尾鱼回主养模式及其与凡纳滨对虾(Penaeus vannamei)混养模式池塘水体不同时空的水质指标以及门、属水平微生物群落结构的差异。水质分析结果显示,斑点叉尾鱼回与凡纳滨对虾混养模式池塘水体氨态氮(NH3-N)含量、可溶性磷酸盐(PO3-4-P)含量均显著高于斑点叉尾鱼回主养模式(P＜0.05),但在健康养殖阈值内未诱发病害。养殖水体微生物群落分析结果表明,放线菌门(Actinobacteriota)细菌和变形菌门(Proteobacteria)细菌在斑点叉尾鱼回主养塘及其与凡纳滨对虾混养塘水体中相对丰度均占据优势地位。冗余分析(RDA)结果表明,斑点叉尾鱼回主养塘水体微生物群落受总氮(TN)含量、总磷(TP)含量影响显著(P＜0.05),斑点叉尾鱼回与凡纳滨对虾混养塘水体可溶性磷酸盐含量是影响微生物群落的主要环境因子(P＜0.05)。综上,斑点叉尾鱼回与凡纳滨对虾混养系统具有良好的生态互补性和水质稳定性,该模式通过生态位互补机制优化水质,降低水体富营养化,在高密度生态养殖中具有可行性。
Abstract:
To investigate the ecological sustainability of the polyculture model of Ictalurus punctatus and optimize the ecological aquaculture system, this study compared the spatiotemporal characteristics of water quality indicators and the differences in microbial community structure at phylum and genus levels between the monoculture model of I. punctatus and its polyculture model with Penaeus vannamei, using national standard water quality testing methods and 16S rRNA high-throughput sequencing technology. Water quality analysis revealed that the polyculture system exhibited significantly higher levels of ammonium nitrogen and soluble phosphate compared with the monoculture system (P＜0.05). However, all values remained within the healthy aquaculture thresholds, and no disease outbreaks were observed. Microbial community analysis indicated that Actinobacteriota and Proteobacteria were the dominant phyla in both systems. Redundancy analysis further revealed that the microbial community in monoculture ponds was significantly influenced by total nitrogen content and total phosphorus content (P<0.05), whereas soluble phosphate content was the primary environmental factor affecting the microbial community in polyculture ponds (P<0.05). In summary, the I. punctatus-P. vannamei polyculture system exhibits strong ecological complementarity and water quality stability. This model enhances water quality and reduces eutrophication through niche complementarity mechanisms, demonstrating its feasibility in high-density pond aquaculture.

参考文献/References:

[1]钟立强,王明华,陈校辉,等. 江苏斑点叉尾鱼回产业现状及发展战略思考[J]. 中国农学通报,2021,37(17):137-143.
[2]Zhong L Q, Song C, Chen X H, et al. Channel catfish in China:historical aspects,current status,and problems[J]. Aquaculture,2016,465:367-373.
[3]蔡焰值,杨德忠,雷晓中,等. 斑点叉尾鱼回亲鱼池塘培育关键技术的研究[J]. 水生态学杂志,2011,32(1):145-148.
[4]蔡焰值,雷晓中,汪亮,等. 斑点叉尾鱼回重要疾病发病原因及预防措施[J]. 湖北农业科学,2010,49(11):2867-2872.
[5]Zhong L Q, Li D M, Wang M H, et al. Dynamics of the bacterial community in a channel catfish nursery pond with a cage-pond integration system[J]. Canadian Journal of Microbiology,2018,64(12):954-967.
[6]张世勇,陈校辉,王明华,等. 斑点叉尾鱼回Cbx基因家族的全基因组鉴定与进化分析[J]. 基因组学与应用生物学,2019,38(4):1488-1496.
[7]钟立强,王明华,张世勇,等. 南京地区斑点叉尾鱼回养殖池塘水体微生物群落结构研究[J]. 农业环境科学学报,2020,39(7):1594-1604.
[8]钟立强,李冰,王明华,等. 斑点叉尾鱼回养殖池塘底泥微生物群落结构特征及其影响因素[J]. 中国水产科学,2020,27(8):893-905.
[9]盖建军,郭闯,陈焕根,等. 3种不同斑点叉尾鱼回养殖模式的水质比较分析(下)[J]. 科学养鱼,2021(2):42-43.
[10]Kochi K, Kobayashi M, Hirotaka S. Feeding habits of Ictalurus punctatus in the downstream section of Nunome Dam reservoir in Japan[J]. Landscape and Ecological Engineering,2021,17(4):563-569.
[11]王明华,姜虎成,陈校辉,等. 斑点叉尾鱼回几种健康养殖模式总结[J]. 水产养殖,2020,41(8):61-63.
[12]徐文彦,赵永军,张慧. 斑点叉尾鱼回、团头鲂池塘混养高产试验[J]. 水产科学,2004,23(10):22-24.
[13]Yuan D R, Yi Y, Yakupitiyage A, et al. Effects of addition of red tilapia (Oreochromis spp.) at different densities and sizes on production,water quality and nutrient recovery of intensive culture of white shrimp (Litopenaeus vannamei) in cement tanks[J]. Aquaculture,2010,298(3/4):226-238.
[14]徐纪萍,高雪忠,叶军强. 上海市奉贤地区淡水池塘凡纳滨对虾和鱼类混养模式探讨[J]. 水产科技情报,2017,44(3):157-161.
[15]Wang Y Y, Xu G C, Nie Z J, et al. Growth performance of bluntnose black bream,channel catfish,yellow catfish,and largemouth bass reared in the In-pond raceway recirculating culture system[J]. North American Journal of Aquaculture,2019,81(2):153-159.
[16]袁新程,施永海,谢永德,等. 混养凡纳滨对虾对草鱼消化、抗氧化、非特异性免疫及代谢能力的影响[J]. 上海农业学报,2024,40(4):92-98.
[17]范秀芬,张光贵,谢德友,等. 斑点叉尾鱼回、南美白对虾混养池塘轮茬养殖太湖青虾技术[J]. 水产养殖,2008,29(3):25.
[18]樊振中,刘维汉,韩克志,等. 南美白对虾与斑点叉尾鱼回、草鱼混养技术试验[J]. 中国水产,2015(6):66-68.
[19]冯伟业,赵一杰,王紫阳,等. 盐碱水池塘鱼虾混养模式构建与养殖效益分析[J]. 中国水产,2023(1):98-100.
[20]葛培,赵佳玉,张弥,等. 淡水养殖池塘CO2通量多时间尺度变化特征及其影响要素[J]. 农业环境科学学报,2024,43(1):190-201.
[21]Musa M, Mahmudi M, Arsad S, et al. Interrelationship and determining factors of water quality dynamics in whiteleg shrimp ponds in tropical eco-green aquaculture system[J]. Journal of Ecological Engineering,2023,24(1):19-27.
[22]Chen F D, Qiu T L, Xu J P, et al. Rapid real-time prediction techniques for ammonia and nitrite in high-density shrimp farming in recirculating aquaculture systems[J]. Fishes,2024,9(10):386.
[23]Nurhasanah A, Rahardja B S, Prayogo. Effect of probiotics in recirculating aquaculture system (RAS) on the concentration of ammonia,nitrite,and nitrate in the aquaculture of catfish (Clarias sp.)[J]. IOP Conference Series:Earth and Environmental Science,2023,1273(1):012018.
[24]Hoang M N, Nguyen P N, Bossier P. Water quality,animal performance,nutrient budgets and microbial community in the biofloc-based polyculture system of white shrimp,Litopenaeus vannamei and gray mullet,Mugil cephalus[J]. Aquaculture,2020,515:734610.
[25]Duan Y F, Tang Y P, Huang J H, et al. Changes in the microbial community of Litopenaeus vannamei larvae and rearing water during different growth stages after disinfection treatment of hatchery water[J]. Journal of Microbiology,2020,58(9):741-749.
[26]Molinas-Vera M, Ferreira-Sanabria G, PeA P, et al. The Paraguayan gut microbiome contains high abundance of the Phylum Actinobacteriota and reveals the influence of health and lifestyle factors[J]. Gut Microbes Reports,2024,1(1):2332988.
[27]Wang Z K, Zhang S L, Li C L, et al. Restoration via the aggregate spray-seeding technique affected the soil proteobacteria on an uninhabited island:community structure,metabolic function,nutritional type,and life strategy[J]. Global Ecology and Conservation,2024,53:03009.
[28]Cao D, Chen W H, Xiang Y P, et al. The efficiencies of inorganic mercury bio-methylation by aerobic bacteria under different oxygen concentrations[J]. Ecotoxicology and Environmental Safety,2021,207:111538.
[29]Liu L M, Wang S S, Chen J F. Anthropogenic activities change the relationship between microbial community taxonomic composition and functional attributes[J]. Environmental Microbiology,2021,23(11):6663-6675.
[30]Liu Q, Lei X J, Li J N, et al. Microbial communities and nitrogen cycling in Litopenaeus vannamei and Mercenaria mercenaria polyculture ponds[J]. Aquaculture Reports,2023,33:101769.
[31]Kündgen M, Jogler C, Kallscheuer N. Substrate utilization and secondary metabolite biosynthesis in the Phylum Planctomycetota[J]. Applied Microbiology and Biotechnology,2025,109(1):123.
[32]魏亚茹,王怡静,马巧丽,等. 粉粒氨氮和水分影响浮霉菌门群落的空间分化[J]. 微生物学通报,2020,47(9):2732-2745.
[33]Zhao Z G, Xu Q Y, Luo L, et al. Effect of bio-floc on water quality and the production performance of bottom and filter feeder carp fed with different protein levels in a pond polyculture system[J]. Aquaculture,2021,531:735906.
[34]曹煜成,文国樑,李卓佳,等. 池塘水体微生物群落代谢活性的动态变化及其与水质的关系[J]. 安全与环境学报,2015,15(1):280-284.
[35]Li J N, Liu G B, Liu Q, et al. Microbial community dynamics and its correlation with environmental factors in the water of polyculture ponds containing Penaeus japonicus,Portunus trituberculatus and Sinonovacula constricta[J]. Aquatic Ecology,2023,57(2):263-279.
[36]Alam M M, Jrgensen N O G, Bass D, et al. Potential of integrated multitrophic aquaculture to make prawn farming sustainable in Bangladesh[J]. Frontiers in Sustainable Food Systems,2024,8:1412919.
[37]Costa J, De Freitas R A, Neto M P N, et al. Integrated multitrophic aquaculture improves the production performance of Amazonian fishes in nursery phase[J]. Aquaculture,2025,600:742240.
[38]赵宇曦,刘兴国,周润锋,等. 池塘多营养级养殖水体的初级生产力及影响因子分析[J]. 渔业现代化,2022,49(6):91-99.
[39]蒋葛,黎慧,沈辉,等. 基于生态沟渠的凡纳滨对虾小型温棚养殖尾水净化技术效能分析[J]. 浙江海洋大学学报(自然科学版),2021,40(6):518-524.
[40]呙金亮,李奎,孙静妤,等. 复合生态沟渠对池塘养殖尾水和稻田退水的净化效果[J]. 水土保持通报,2022,42(6):206-213.
[41]Lu J X, Guo Z Z, Kang Y, et al. Recent advances in the enhanced nitrogen removal by oxygen-increasing technology in constructed wetlands[J]. Ecotoxicology and Environmental Safety,2020,205:111330.
[42]Roy S M, Choi H, Kim T. Review of state-of-the-art improvements in recirculating aquaculture systems:insights into design,operation,and statistical modeling approaches[J]. Aquaculture,2025,605:742545.
[43]熊莹槐,罗晓春,钱雪桥,等. 复合酶制剂和复合菌制剂对凡纳滨对虾生长、体生化组成及能量收支的影响[J]. 海洋湖沼通报,2022,44(6):41-48.

备注/Memo

备注/Memo:
收稿日期：2025-08-11基金项目：国家重点研发计划项目(2023YFD2400500)作者简介：丁振源(1999-),男,山东潍坊人,硕士研究生,研究方向为生态渔业。(E-mail)m230501228@st.shou.edu.cn通讯作者：管卫兵,(E-mail)wbguan@shou.edu.cn
更新日期/Last Update: 2026-08-21