[1]张溪,沈素素,薛利红.磷添加对长期缺磷稻田土壤磷转化和甲烷排放的影响[J].江苏农业学报,2026,42(07):1387-1398.[doi:doi:10.3969/j.issn.1000-4440.2026.07.010]
　ZHANG Xi,SHEN Susu,XUE Lihong.Impacts of phosphorus addition on phosphorus transformation and CH4 emission in long-term phosphorus-deficient paddy soils[J].,2026,42(07):1387-1398.[doi:doi:10.3969/j.issn.1000-4440.2026.07.010]
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磷添加对长期缺磷稻田土壤磷转化和甲烷排放的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1387-1398
栏目:
耕作栽培·资源环境
出版日期:
2026-07-31

文章信息/Info

Title:
Impacts of phosphorus addition on phosphorus transformation and CH4 emission in long-term phosphorus-deficient paddy soils
作者:
张溪1; 沈素素1; 2; 薛利红1; 2
(1.江苏省农业科学院农业资源与环境研究所/农业农村部长江下游平原农业环境重点实验室,江苏南京210014；2.南京农业大学资源与环境科学学院,江苏南京211800)
Author(s):
ZHANG Xi1; SHEN Susu1; 2; XUE Lihong1; 2
(1.Institute of Agricultural Resources and Environment, Jiangsu Academy of Agricultural Sciences/Key Laboratory of Agro-Environment in Downstream of Yangtze Plain, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China;2.College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 211800, China)
关键词:
长期缺磷; 稻田; 磷; 甲烷; 产甲烷菌; 甲烷氧化菌
Keywords:
long-term phosphorus-deficiency; paddy soil; phosphorus; methane; methanogen; methanotroph
分类号:
S511; S153.6
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.010
文献标志码:
A
摘要:
本研究以长期不施磷稻田土壤为研究对象,通过盆栽试验设置施磷处理(NKP)与不施磷处理(NK),系统分析磷添加对缺磷土壤磷素形态、磷转化微生物、甲烷(CH4)产生及水稻生长的影响。结果表明,磷肥投入可显著增加土壤全磷含量和有效磷含量（P<0.05）；土壤无机磷以铁结合态磷(Fe-P)和钙结合态磷(Ca-P)为主,有机磷以活性和中活性为主,各形态磷绝对含量随磷肥投入而增加,但其占全磷比例降低。施磷处理抑制了土壤磷酸酶活性及磷转化功能基因表达,且分蘖期的抑制远超成熟期。施磷处理虽未改变产甲烷菌群落结构,但驱动甲烷氧化菌中甲基单胞菌属(Methylomonas)菌显著增殖并抑制甲基杆菌属(Methylobacter)菌增殖,这可能是降低水稻分蘖期稻田土壤甲烷排放峰值的关键因子。相关性分析结果表明,甲烷排放量与闭蓄态磷含量和活性有机磷含量呈显著负相关（P<0.05）,这可能与闭蓄态磷的“物理屏障”效应阻碍产甲烷底物扩散,以及活性有机磷对甲烷氧化菌的营养支持有关。此外,与不施磷处理相比施磷处理显著提升水稻产量和植株吸磷量（P<0.05）。综上,在长期缺磷稻田中施用磷肥可通过调控土壤磷循环与甲烷氧化菌群落实现水稻分蘖期稻田土壤甲烷排放通量降低与水稻增产。
Abstract:
This study took long-term phosphorus-deficient paddy soil as the research object. Pot experiments were conducted with a phosphorus application treatment (NKP) and a no-phosphorus application treatment (NK). The effects of phosphorus addition on phosphorus fractions, phosphorus-transforming microorganisms, methane (CH4) production and rice growth in phosphorus-deficient soil were systematically analyzed. The results showed that phosphorus addition significantly increased soil total phosphorus and available phosphorus contents (P<0.05). Soil inorganic phosphorus was dominated by iron-bound phosphorus (Fe-P) and calcium-bound phosphorus (Ca-P), while organic phosphorus was composed mainly of labile and moderately labile fractions. The absolute content of each phosphorus fraction increased with phosphorus addition, but its proportion to total phosphorus decreased. Phosphorus addition inhibited soil phosphatase activity and the expression of phosphorus-transforming functional genes, and the inhibition was much stronger at the tillering stage than at the maturity stage. Phosphorus addition did not alter the community structure of methanogens, but it drove a significant proliferation of Methylomonas and inhibited the proliferation of Methylobacter among methanotrophs, which may be a key factor in reducing the peak methane emission from paddy soil at the tillering stage. Correlation analysis indicated that methane emission was significantly negatively correlated with occluded phosphorus content and labile organic phosphorus content (P<0.05). This may be related to the “physical barrier” effect of occluded phosphorus in hindering the diffusion of methanogenic substrates, and to the nutritional support provided by labile organic phosphorus for methanotrophs. Furthermore, compared with the no-phosphorus treatment, phosphorus addition significantly increased rice yield and plant phosphorus uptake (P<0.05). In conclusion, phosphorus addition to long-term phosphorus-deficient paddy soil can reduce methane emission flux from the paddy soil at the tillering stage and increase rice yield by regulating soil phosphorus cycling and the methanotrophic community.

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备注/Memo

备注/Memo:
收稿日期:2025-08-08基金项目:国家重点研发计划项目(2021YFD1700803)；国家自然科学基金项目(41877087)作者简介:张溪(1996-),男,江苏涟水人,博士,助理研究员,研究方向为农田养分管理与面源污染阻止与控制。(E-mail)zhangxi@jaas.ac.cn通讯作者:薛利红,(E-mail)xuelihong@jaas.ac.cn
更新日期/Last Update: 2026-08-21