[1]杨艺,柯甫志,聂振朋,等.柑橘间座壳菌全基因组非经典分泌蛋白预测及致病相关性分析[J].江苏农业学报,2026,42(07):1360-1369.[doi:doi:10.3969/j.issn.1000-4440.2026.07.007]
　YANG Yi,KE Fuzhi,NIE Zhenpeng,et al.Genome-wide prediction and pathogenic analysis of non-classical secreted proteins of Diaporthe citri[J].,2026,42(07):1360-1369.[doi:doi:10.3969/j.issn.1000-4440.2026.07.007]
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柑橘间座壳菌全基因组非经典分泌蛋白预测及致病相关性分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1360-1369
栏目:
植物保护
出版日期:
2026-07-31

文章信息/Info

Title:
Genome-wide prediction and pathogenic analysis of non-classical secreted proteins of Diaporthe citri
作者:
杨艺; 柯甫志; 聂振朋; 王罗云; 崔长江; 黄秀; 蒲占湑; 徐建国; 孙立方
(浙江省农业科学院/浙江省柑橘研究所/国家柑橘品种改良中心浙江分中心,浙江台州318020)
Author(s):
YANG Yi; KE Fuzhi; NIE Zhenpeng; WANG Luoyun; CUI Changjiang; HUANG Xiu; PU Zhanxu; XU Jianguo; SUN Lifang
(Zhejiang Academy of Agricultural Sciences/Citrus Research Institute of Zhejiang Province/Zhejiang Branch of National Citrus Variety Improvement Center, Taizhou 318020, China)
关键词:
柑橘间座壳菌; 柑橘黑点病; 非经典分泌蛋白; 致病基因
Keywords:
Diaporthe citri; citrus melanose; non-classical secreted proteins; disease-causing gene
分类号:
S436.66
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.007
文献标志码:
A
摘要:
由柑橘间座壳菌(Diaporthe citri)诱导的黑点病是全球柑橘生产面临的重要病害之一,严重降低了柑橘的外观品质和经济价值。分泌蛋白参与病原菌致病过程,但目前相关研究多关注经典分泌蛋白,而对非经典分泌蛋白知之甚少。本研究基于柑橘间座壳菌全基因组序列,分别利用TMHMM 2.0、WoLF PSORT、SignalP 5.0、SecretomeP-1.0、dbCAN、eggNOG-mapper和PHI-BLAST等生物信息学工具,开展非经典分泌蛋白的预测及功能分析,并利用实时荧光定量逆转录聚合酶链反应(qRT-PCR)对其中11个候选基因在柑橘间座壳菌侵染过程中的转录水平进行分析。结果表明,从柑橘间座壳菌全基因组编码的15 761个蛋白质中预测到302个候选非经典分泌蛋白,其中,11个为潜在的致病力增强非经典分泌蛋白。qRT-PCR验证结果表明,2个候选基因(INS49_010083和INS49_015019)在柑橘间座壳菌侵染感病种质鸡尾葡萄柚果实过程中显著上调表达,推测它们可能参与柑橘间座壳菌致病过程。本研究结果为开展柑橘间座壳菌致病机制研究及柑橘抗黑点病种质精准培育奠定理论依据。
Abstract:
Citrus melanose, caused by Diaporthe citri, is one of the important diseases affecting citrus cultivation worldwide, which significantly reduces the appearance quality and economic value of citrus. Secretory proteins play crucial roles in the pathogen infection process. However, current research mainly focuses on classical secretory proteins, with limited attention paid to non-classical secretory proteins. In this study, based on the whole-genome sequence of D. citri, non-classical secreted proteins were predicted by using TMHMM 2.0, WoLF PSORT, SignalP 5.0 and SecretomeP-1.0, and their functional annotations were analyzed by using dbCAN, eggNOG-mapper and PHI-BLAST. Moreover, the transcription levels of 11 candidate genes during D. citri infection were analyzed by using quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The results showed that 302 candidate non-classical secretory proteins were predicted from the 15 761 encoded proteins, of which 11 were potential virulence-enhancing proteins. Additionally, two candidate genes (INS49_010083 and INS49_015019) were significantly upregulated during the infection of fruits of the susceptible germplasm Cocktail grapefruit by Diaporthe citri, suggesting that they may be involved in the pathogenic process of D. citri. These results provide a theoretical basis for elucidating the pathogenic mechanisms of D. citri and conducting precision breeding of citrus varieties resistant to citrus melanose.

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备注/Memo

备注/Memo:
收稿日期：2025-08-21基金项目：浙江省农业新品种选育重大专项(2021C02066-1)；浙江省农业重大技术协同推广计划项目(2024ZDXT04-1)；国家重点研发计划项目(2024YFD2300804)；国家现代农业(柑橘)产业技术体系项目(CARS-26)作者简介：杨艺(1995-),女,江西南昌人,博士,助理研究员,主要从事柑橘品质分子育种研究。(E-mail) jiayouziyi@126.com通讯作者：孙立方,(E-mail)sun6286855@126.com
更新日期/Last Update: 2026-08-21