[1]任昂彦,陈涛,李晓莎,等.向日葵COBRA家族基因的全基因组鉴定与分析[J].江苏农业学报,2026,42(07):1338-1348.[doi:doi:10.3969/j.issn.1000-4440.2026.07.005]
　REN Angyan,CHEN Tao,LI Xiaosha,et al.Genome-wide identification and analysis of COBRA family genes in sunflower[J].,2026,42(07):1338-1348.[doi:doi:10.3969/j.issn.1000-4440.2026.07.005]
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向日葵COBRA家族基因的全基因组鉴定与分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1338-1348
栏目:
遗传育种·生理生化
出版日期:
2026-07-31

文章信息/Info

Title:
Genome-wide identification and analysis of COBRA family genes in sunflower
作者:
任昂彦1; 陈涛1; 李晓莎1; 贺江1; 杨华1; 高进1; 刘爱萍2; 陈环宇1; 戚永奎1
(1.江苏沿海地区农业科学研究所,江苏盐城224002；2.建湖县芦沟镇农业农村办公室,江苏建湖224711)
Author(s):
REN Angyan1; CHEN Tao1; LI Xiaosha1; HE Jiang1; YANG Hua1; GAO Jin1; LIU Aiping2; CHEN Huanyu1; QI Yongkui1
(1.Institute of Agricultural Sciences in the Coastal District of Jiangsu Province, Yancheng 224002, China;2.Agriculture and Rural Affairs Office of Lugou Town, Jianhu County, Jianhu 224711, China)
关键词:
向日葵; COBRA蛋白; 纤维素; 细胞壁
Keywords:
sunflower; COBRA protein; cellulose; cell wall
分类号:
S565.5
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.005
文献标志码:
A
摘要:
COBRA蛋白是一类糖基磷脂酰肌醇(GPI)锚定蛋白,在植物细胞壁生物合成、细胞分裂、细胞扩张和防御反应等过程中发挥重要作用。本研究在向日葵全基因组数据中鉴定出不均匀分布在11条染色体上的16个COBRA基因家族成员。基于生物信息学分析,向日葵COBRA家族基因可划分为2个亚家族,二者在结构特征上存在显著差异,包括基因结构以及编码的COBRA蛋白关键保守结构域（半胱氨酸-半胱氨酸-缬氨酸-丝氨酸，CCVS）变异、亲疏水模式分化、GPI锚定修饰位点分化等。启动子顺式作用元件分析发现,该家族基因均含2种以上参与逆境胁迫响应、激素信号传导或发育调控相关的顺式作用元件。蛋白质互作预测结果显示, HaCOBRA10蛋白和HaCOBRA14蛋白均与2个纤维素合成酶蛋白(CESA4、CESA8)及2个糖基转移酶蛋白(IRX3、IRX9)互作。组织差异表达和胁迫响应分析发现,该家族基因在生长发育调控和逆境胁迫响应中可能具有功能多样性。本研究结果为向日葵细胞壁形成机制研究及抗逆育种提供了新靶点,并为进一步探究COBRA蛋白在植物中的生物学功能奠定了理论基础。
Abstract:
COBRA proteins are a class of glycosyl-phosphatidylinositol (GPI)-anchored proteins that play important roles in plant cell wall biosynthesis, cell division, cell expansion, and defense responses. In this study, 16 COBRA genes unevenly distributed across 11 chromosomes were identified in the sunflower whole-genome. Based on bioinformatics analysis, this family was classified into two subfamilies, with significant differences in structural features, including gene structure, variations in the key conserved domain (cysteine-cysteine-valine-serine, CCVS), divergence in hydrophobicity/hydrophilicity patterns, and divergence in GPI-anchoring modification sites. Analysis of promoter cis-acting elements revealed that all members contained at least two types of cis-acting elements involved in stress response, hormone signaling, or developmental regulation. The protein-protein interaction prediction results showed that both HaCOBRA10 and HaCOBRA14 interacted with two cellulose synthases (CESA4, CESA8) and two glycosyltransferases (IRX3, IRX9). Tissue-specific expression and stress response analyses suggested that genes in this family may exhibit functional diversity in growth, development, and stress adaptation. This study provides new targets for research on cell wall formation mechanism and stress resistance breeding in sunflower, and lays a theoretical foundation for further investigation into the biological functions of COBRA proteins in plants.

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备注/Memo

备注/Memo:
收稿日期：2025-07-30基金项目：江苏省盐城市应用基础研究计划项目(YCBK2025063、YCBK2025071)；农业农村部沿海盐碱地农业科学观测实验站开放课题(YHS202105、YHS202306)；江苏省农业科技自主创新基金项目[CX(23)1040]；江苏省基础研究计划青年基金项目(BK20240495)作者简介：任昂彦(1989-),女,山东菏泽人,博士,助理研究员,主要从事经济作物遗传育种研究。(E-mail)ray0918@163.com通讯作者：戚永奎,(E-mail)qiyongkuiworld@sina.cn
更新日期/Last Update: 2026-08-21