[1]王新宇,刘卓君,郭子浩,等.普通小麦TaSeBP基因表达与自然等位变异分析[J].江苏农业学报,2026,42(07):1317-1325.[doi:doi:10.3969/j.issn.1000-4440.2026.07.003]
　WANG Xinyu,LIU Zhuojun,GUO Zihao,et al.Expression and natural allelic variation analysis of TaSeBP gene in common wheat[J].,2026,42(07):1317-1325.[doi:doi:10.3969/j.issn.1000-4440.2026.07.003]
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普通小麦TaSeBP基因表达与自然等位变异分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1317-1325
栏目:
遗传育种·生理生化
出版日期:
2026-07-31

文章信息/Info

Title:
Expression and natural allelic variation analysis of TaSeBP gene in common wheat
作者:
王新宇1; 刘卓君1; 郭子浩1; 史小霞1; 郝昱宁1; 韦小龙1; 杨博慧1; 贾举庆1; 杨珍平1; 吕晋慧1; 姜亮1; 2; 张春来1
(1.山西农业大学农学院/省部共建黄土高原作物高效生产协同创新中心,山西太谷030801；2.山西后稷实验室,山西太原030031)
Author(s):
WANG Xinyu1; LIU Zhuojun1; GUO Zihao1; SHI Xiaoxia1; HAO Yuning1; WEI Xiaolong1; YANG Bohui1; JIA Juqing1; YANG Zhenping1; LYU Jinhui1; JIANG Liang1; 2; ZHANG Chunlai1
(1.College of Agriculture, Shanxi Agricultural University/Collaborative Innovation Center for Efficient Crop Production on the Loess Plateau, Taigu 030801, China;2.Shanxi Houji Laboratory, Taiyuan 030031, China)
关键词:
普通小麦; 硒结合蛋白; 硒添加; 寒冷胁迫; 自然等位变异
Keywords:
common wheat (Triticum aestivum L.); selenium-binding protein; selenium supplement; freezing stress; natural allelic variation
分类号:
S512.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.003
文献标志码:
A
摘要:
本研究应用生物信息和分子生物学方法分析小麦SeBP基因的表达和自然等位变异,为筛选和创制富硒小麦种质资源提供理论依据和方法。对小麦不同品种SeBP基因表达量分析及逆境胁迫处理研究的结果表明,TaSeBP-3D的相对表达量最高；此外TaSeBP响应硒添加和寒冷胁迫,Huaimai18诱导相对表达量上调,Jinghe1号相对表达量降低；对候选启动子区域的顺式作用元件分析发现,存在脱落酸响应、赤霉素响应等元件,推测SeBP基因与植株生长发育、响应逆境胁迫有关；对小麦自然群体DNA变异分析结果表明,TaSeBP存在多个错义突变位点和单核苷酸插入删除变异；通过对小麦SeBP系统发育进化分析发现其存在单双子叶植物分化。本研究结果可为深入研究TaSeBP基因功能及分子育种应用提供参考。
Abstract:
This study applied bioinformatics and molecular biology methods to analyze the expression and natural allelic variation of the wheat SeBP gene, providing theoretical basis and methods for screening and developing selenium-rich wheat germplasm resources. Through the analysis of expression levels of SeBP gene in different wheat varieties and studies under stress treatments, the results showed that TaSeBP-3D had the highest expression level. Furthermore, TaSeBP responded to selenium supplementation and cold stress, with Huaimai 18 showing an upregulation in relative expression, while Jinghe No.1 showed a decrease in relative expression. Analysis of cis-acting elements in the candidate promoter region revealed the presence of abscisic acid-responsive, gibberellin-responsive elements, suggesting a relationship with plant growth and development and response to stress. DNA variation analysis in natural wheat populations indicated that TaSeBP had multiple missense mutation sites and single nucleotide insertion-deletion variations. Phylogenetic and evolutionary analysis of wheat SeBP revealed differentiation between monocotyledonous and dicotyledonous plants. The results of this study can provide a reference for in-depth research on TaSeBP gene function and its application in molecular breeding.

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备注/Memo

备注/Memo:
收稿日期：2025-07-13基金项目：国家自然科学基金项目(31971944)；山西省教育厅研究生教育创新计划实践创新项目(2023SJ109)；山西省教育厅专业建设项目(J20220211)；山西农谷建设科研专项(SXNGJSKYZX201702)作者简介：王新宇(1998-),男,陕西宝鸡人,硕士研究生,研究方向为小麦分子育种。(E-mail)2747009861@qq.com通讯作者：张春来,(E-mail)chunlaiz@hotmail.com
更新日期/Last Update: 2026-08-21