[1]邓亚淋,代惠珍,谢明月,等.基于非靶向代谢组学解析不同水稻品种的品质差异[J].江苏农业学报,2026,42(07):1305-1316.[doi:doi:10.3969/j.issn.1000-4440.2026.07.002]
　DENG Yalin,DAI Huizhen,XIE Mingyue,et al.Untargeted-metabolomics dissection of quality differences among rice cultivars[J].,2026,42(07):1305-1316.[doi:doi:10.3969/j.issn.1000-4440.2026.07.002]
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基于非靶向代谢组学解析不同水稻品种的品质差异()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年07期
页码:
1305-1316
栏目:
遗传育种·生理生化
出版日期:
2026-07-31

文章信息/Info

Title:
Untargeted-metabolomics dissection of quality differences among rice cultivars
作者:
邓亚淋1; 代惠珍1; 谢明月1; 茹颖晶1; 唐菊1; 曾珍1; 周敏逍1; 张声浩1; 刘孟泽1; 吴合周2; 胡景涛1
(1.重庆三峡科技大学生物与食品工程学院,重庆404000；2.湖南桃花源农业科技股份有限公司,湖南常德415000)
Author(s):
DENG Yalin1; DAI Huizhen1; XIE Mingyue1; RU Yingjing1; TANG Ju1; ZENG Zhen1; ZHOU Minxiao1; ZHANG Shenghao1; LIU Mengze1; WU Hezhou2; HU Jingtao1
(1.College of Biology and Food Engineering, Chongqing Sanxia University of Science and Technology, Chongqing 404000, China;2.Hunan Taohuayuan Agricultural Science and Technology Co., Ltd., Changde 415000, China)
关键词:
风味; 品质; 储藏; 水稻; 非靶向代谢组学
Keywords:
flavor; quality; storage; rice; untargeted metabolomics
分类号:
S511.2+1
DOI:
doi:10.3969/j.issn.1000-4440.2026.07.002
文献标志码:
A
摘要:
为比较相同储藏温度下不同品种稻米中品质相关代谢物的构成差异,采用超高效液相色谱-串联质谱(UPLC-MS/MS)非靶向代谢组学技术,对籼稻桃湘优188(TXY188)和五优308(WY308)储藏前与恒温条件储藏90 d后的差异代谢产物进行分析并解析其代谢通路。结果表明,通过UPLC-MS/MS共检测出3 055种代谢物。储藏初期,与WY308相比有239种代谢物在TXY188中高度积累,丁香亭、香草醛、肉桂酸和松柏醛等代谢物在TXY188中显著富集。储藏90 d后,香草醛、肉桂酸和松柏醛等代谢物含量仍高于WY308。京都基因与基因组百科全书(KEGG)富集分析结果显示,类黄酮生物合成和苯丙烷代谢是主要代谢通路。本研究从风味代谢物动态变化角度,为稻谷储藏过程中品质的调控提供了数据支持。
Abstract:
To compare the differences in the composition of quality-related metabolites in rice among varieties stored at the same temperature, untargeted metabolomics approach based on ultraperformance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) was used to analyze differential metabolites and elucidate metabolic pathways in the indica rice varieties, Taoxiangyou 188 (TXY188) and Wuyou 308 (WY308), before storage and after 90 d of storage under constant-temperature conditions. The results showed that a total of 3 055 metabolites were detected by UPLC-MS/MS. At the initial stage of storage, 239 metabolites were accumulated at higher levels in TXY188 than in WY308, and metabolites such assyringetin, vanillin, cinnamic acid, and coniferaldehyde were significantly enriched in TXY188. After 90 d of storage, the contents of metabolites such as vanillin, cinnamic acid and coniferaldehyde remained higher in TXY188 than in WY308. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis revealed that flavonoid biosynthesis and phenylpropanoid metabolism were the major metabolic pathways involved. This study provides data support for regulation of grain quality during rice storage from the perspective of dynamic changes in flavor-related metabolites.

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备注/Memo

备注/Memo:
收稿日期：2026-02-25基金项目：西南作物基因资源发掘与利用国家重点实验室开放课题(SKL-KF202424)；重庆三峡科技大学研究生科研创新项目(YJSKY25027)作者简介：邓亚淋(1999-),女,重庆人,硕士研究生,研究方向为作物品质及逆境生物学调控。(E-mail)dengyalin0903@163.com。代惠珍为共同第一作者。通讯作者：胡景涛,(E-mail)20180016@sanxiau.edu.cn
更新日期/Last Update: 2026-08-21