[1]田然旗,成梦凯,杨姣姣,等.大白菜MBF1家族基因鉴定及高温或低温胁迫下的表达分析[J].江苏农业学报,2026,42(05):1015-1026.[doi:doi:10.3969/j.issn.1000-4440.2026.05.015]
　TIAN Ranqi,CHENG Mengkai,YANG Jiaojiao,et al.Identification of the MBF1 family genes in Chinese cabbage and expression analysis under high- or low-temperature stress[J].,2026,42(05):1015-1026.[doi:doi:10.3969/j.issn.1000-4440.2026.05.015]
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大白菜MBF1家族基因鉴定及高温或低温胁迫下的表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年05期
页码:
1015-1026
栏目:
园艺
出版日期:
2026-05-31

文章信息/Info

Title:
Identification of the MBF1 family genes in Chinese cabbage and expression analysis under high- or low-temperature stress
作者:
田然旗1; 成梦凯1; 杨姣姣1; 张鼎晨1; 毛学达1; 胡琳莉1; 2
(1.甘肃农业大学园艺学院,甘肃兰州730070；2.干旱生境作物学国家重点实验室,甘肃兰州730070)
Author(s):
TIAN Ranqi1; CHENG Mengkai1; YANG Jiaojiao1; ZHANG Dingchen1; MAO Xueda1; HU Linli1; 2
(1.College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China;2.State Key Laboratory of Aridland Crop Science, Lanzhou 730070, China)
关键词:
大白菜; MBF1家族基因; 生物信息学分析; 基因表达分析; 高温或低温胁迫响应
Keywords:
Chinese cabbage; MBF1 family genes; bioinformatics analysis; gene expression analysis; response to high or low temperature stress
分类号:
S634.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.05.015
文献标志码:
A
摘要:
多蛋白桥梁因子(Multiprotein bridging factor 1,MBF1)作为一类转录共激活因子,在植物响应高温胁迫和低温胁迫过程中发挥重要作用。本研究在大白菜全基因组中系统鉴定MBF1家族基因,并对其分子特征进行分析。通过生物信息学手段对该家族基因的结构、染色体定位、顺式作用元件、种内与种间共线性关系及编码蛋白质理化性质、亚细胞定位、蛋白质结构进行全面分析；并基于转录组测序(RNA-Seq)数据结合实时荧光定量逆转录PCR(qRT-PCR)技术,解析其在高温和低温胁迫下的表达模式。结果表明,大白菜MBF1家族基因含有6个成员(分别命名为BrMBF1-01～BrMBF1-06),分布于5条染色体上,其编码的蛋白质氨基酸数量介于127 aa至458 aa,相对分子量为14 098.37～50 794.78,等电点位于9.25～10.16。从该家族基因启动子中鉴定出激素响应、胁迫响应、昼夜节律调控3大类顺式作用元件。大白菜MBF1家族基因编码的蛋白质二级结构均包含α-螺旋、β-转角、延伸链及无规则卷曲，二级结构组成高度保守,三级结构构象存在明显多样性。3对大白菜MBF1基因存在片段重复,同时大白菜MBF1基因与拟南芥MBF1基因之间存在种间共线性关系。低温胁迫下,大白菜BrMBF1-02、BrMBF1-03基因相对表达量下调,其余4个MBF1基因相对表达量呈先下调后上调趋势；高温胁迫下,除BrMBF1-06基因和BrMBF1-02基因外,另外4个MBF1基因响应迅速,相对表达量显著上调。综上,本研究结果既为大白菜MBF1基因的功能研究奠定了基础,也为其抗高温和低温胁迫分子育种提供了基因资源。
Abstract:
Multiprotein bridging factor 1 (MBF1), as a class of transcriptional coactivators, plays an important role in plant responses to high- and low-temperature stress. In this study, the MBF1 family genes were systematically identified from the whole genome of Chinese cabbage, and their molecular characteristics were analyzed. Using bioinformatics tools, the gene structures, chromosomal locations, cis-acting elements, intraspecific and interspecific collinearity of these family genes, as well as the physicochemical properties, subcellular localization, and protein structures of the encoded proteins, were comprehensively analyzed. Furthermore, based on transcriptome sequencing (RNA-seq) data combined with quantitative real-time reverse transcription PCR (qRT-PCR), their expression patterns under high- and low-temperature stress were analyzed. The results showed that the Chinese cabbage MBF1 family genes contained six members (designated BrMBF1-01 to BrMBF1-06, respectively), which were distributed on five chromosomes. The encoded proteins ranged from 127 aa to 458 aa in amino acid length, with relative molecular masses from 14 098.37 to 50 794.78, and their isoelectric points ranged from 9.25 to 10.16. The promoters of these family genes were found to contain three major categories of cis-acting elements: hormone-responsive, stress-responsive, and circadian rhythm regulatory elements. The secondary structures of the proteins encoded by the Chinese cabbage MBF1 family genes all contained α-helices, β-turns, extended strands, and random coils. The secondary structure composition was highly conserved, while the tertiary structure conformations exhibited significant diversity. Three pairs of MBF1 genes in Chinese cabbage exhibited segmental duplications, and there was interspecific collinearity between Chinese cabbage MBF1 genes and Arabidopsis thaliana MBF1 genes. Under low-temperature stress, the relative expression levels of BrMBF1-02 and BrMBF1-03 genes in Chinese cabbage were downregulated, while the remaining four MBF1 genes first decreased and then increased in their relative expression levels. Under high-temperature stress, except for the BrMBF1-06 and BrMBF1-02 genes, the other four MBF1 genes responded rapidly and showed significantly upregulated relative expression levels. In summary, the results of this study lay a foundation for functional studies of MBF1 genes in Chinese cabbage and also provide genetic resources for molecular breeding for resistance to high-temperature and low-temperature stresses.

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备注/Memo

备注/Memo:
收稿日期：2026-01-25基金项目：甘肃农业大学大学生创新创业项目(202512010)；国家自然科学基金项目(32560727)；干旱生境作物学国家重点实验室主任基金项目(GSCS-2023-Z06)作者简介：田然旗(2004-),男,山东济宁人,本科,研究方向为作物逆境分子生物学。(E-mail)13142207302@163.com通讯作者：胡琳莉,(E-mail)hull@gsau.edu.cn
更新日期/Last Update: 2026-06-17