[1]邝良德,王云端,李钰莹,等.四川麻兔不同泌乳时期乳脂球膜蛋白的差异分析[J].江苏农业学报,2026,42(05):990-1003.[doi:doi:10.3969/j.issn.1000-4440.2026.05.013]
　KUANG Liangde,WANG Yunduan,LI Yuying,et al.Differential analysis of milk fat globule membrane proteins across lactation stages in Sichuan rabbits[J].,2026,42(05):990-1003.[doi:doi:10.3969/j.issn.1000-4440.2026.05.013]
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四川麻兔不同泌乳时期乳脂球膜蛋白的差异分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年05期
页码:
990-1003
栏目:
畜牧兽医·水产养殖
出版日期:
2026-05-31

文章信息/Info

Title:
Differential analysis of milk fat globule membrane proteins across lactation stages in Sichuan rabbits
作者:
邝良德1; 王云端2; 李钰莹1; 刘艳1; 穆雪儿2; 雷岷1; 郑洁1; 郭志强1; 杨锐1; 向雨晗2; 付伟2; 李丛艳1
(1.四川省畜牧科学研究院/畜禽种业四川省重点实验室,四川成都610066；2.西南民族大学畜牧兽医学院/西南民族大学青藏高原动物遗传资源保护与利用教育部重点实验室,四川成都610041)
Author(s):
KUANG Liangde1; WANG Yunduan2; LI Yuying1; LIU Yan1; MU Xue’er2; LEI Min1; ZHENG Jie1; GUO Zhiqiang1; YANG Rui1; XIANG Yuhan2; FU Wei2; LI Congyan1
(1.Sichuan Animal Science Academy/Animal Genetic Breeding and Reproduction Key Laboratory of Sichuan Province, Chengdu 610066, China;2.College of Animal Science and Veterinary Medicine, Southwest Minzu University/Key Laboratory of the Qinghai-Tibet Plateau Animal Genetic Resource Reservation and Utilization of Ministry of Education, Southwest Minzu University, Chengdu 610041, China)
关键词:
四川麻兔; 泌乳期; 乳脂球膜蛋白
Keywords:
Sichuan rabbits; lactation; milk fat globule membrane protein
分类号:
S829.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.05.013
文献标志码:
A
摘要:
乳脂球膜(MFGM)蛋白在脂质代谢、免疫防御及泌乳调控中发挥关键作用。本研究采用非对称轨道无损质量分析器-数据非依赖性采集(Astral-DIA)质谱技术对四川麻兔泌乳前期(MM1)、泌乳中期(MM21)、泌乳后期(MM35)的乳脂球膜蛋白质谱进行分析。共鉴定出2 937种乳脂球膜蛋白,其中1 907种在所有泌乳阶段均存在。泌乳前期与泌乳后期之间存在1 331种差异表达蛋白(DEMP),泌乳中期与泌乳后期之间有458种差异表达蛋白,泌乳前期与泌乳中期间有1 351种差异表达蛋白。通过KEGG通路映射、eggNOG分类注释、Pfam结构域分析及亚细胞定位预测进行多维功能注释,揭示了乳脂球膜蛋白功能在3个泌乳阶段呈现差异性:泌乳前期更关联生物体免疫系统；泌乳中期与泌乳后期功能趋于稳定,主要参与营养代谢。通过优化Mfuzz时序聚类及关联功能注释,解析了特征乳脂球膜蛋白的时间表达模式,同时筛选出8种代表性乳脂球膜蛋白。本研究结果表明,四川麻兔乳脂球膜蛋白具备脂质代谢、囊泡运输、信号调控和免疫保护的复杂功能特征,既为能量代谢提供支持,又可通过免疫通路提高新生幼兔对病原体的防御能力。本研究结果阐明了兔不同泌乳阶段乳汁中特异性蛋白质组学特征及其生物学意义,为后续研究乳脂球膜蛋白在乳汁分泌和新生幼兔发育中的作用提供了重要理论基础。
Abstract:
Milk fat globule membrane (MFGM) proteins play essential roles in lipid metabolism, immune defense, and the regulation of lactation. In this study, the asymmetric track lossless mass analyzer-data-independent acquisition (Astral-DIA) mass spectrometry platform was employed to profile the MFGM proteome of Sichuan rabbits across three lactation stages: early lactation (MM1), mid-lactation (MM21), and late lactation (MM35). A total of 2 937 MFGM proteins were identified, of which 1 907 were consistently present throughout all lactation stages. Comparative analyses revealed 1 331 differentially expressed MFGM proteins (DEMPs) between early lactation and late lactation, 458 DEMPs between mid-lactation and late lactation, and 1 351 DEMPs between early lactation and mid-lactation. Functional annotations based on KEGG pathway analysis, eggNOG classification, Pfam domain identification, and subcellular localization prediction uncovered distinct stage-specific roles of MFGM proteins: those in early lactation were predominantly linked to immune function, while proteins in mid-lactation and late lactation were functionally stable and mainly involved in nutrient metabolism. Time-course expression patterns of characteristic MFGM proteins were further dissected using optimized Mfuzz clustering, and eight representative proteins were identified and functionally characterized. The MFGM proteins of Sichuan rabbits exhibited complex functional characteristics related to lipid metabolism, vesicle trafficking, signal transduction, and immune protection, supporting both energy supply and innate immunity in neonates. This study elucidates the specific proteomic characteristics and biological significance of MFGM proteins during lactation, providing an important foundation for subsequent research on the functional roles of MFGM proteins in milk secretion and development of newborn rabbit pups.

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备注/Memo

备注/Memo:
收稿日期：2025-09-05基金项目：四川省科研院所基本科研项目(SASA202405)；国家现代农业产业技术体系四川兔创新团队项目(SCCXTD-2024-26)；国家兔产业技术体系项目(CARS-43-D-1)；四川省畜禽育种攻关项目(2021YFYZ0033)；四川省财政运行专项(SASA2025CZYX004)作者简介：邝良德(1983-),男,湖南郴州人,硕士,副研究员,研究方向为家兔遗传育种与饲养。(E-mail)215640832@qq.com通讯作者：李丛艳,(E-mail)licongyan0311@sina.com
更新日期/Last Update: 2026-06-17