[1]张明浩,王爱凡,龚林涛,等.芳樟醇合酶基因(LIS)在薰衣草萜类物质合成中的功能分析[J].江苏农业学报,2026,42(04):808-816.[doi:doi:10.3969/j.issn.1000-4440.2026.04.017]
　ZHANG Minghao,WANG Aifan,GONG Lintao,et al.Functional analysis of linalool synthase gene (LIS) in terpenoid synthesis of lavender[J].,2026,42(04):808-816.[doi:doi:10.3969/j.issn.1000-4440.2026.04.017]
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芳樟醇合酶基因(LIS)在薰衣草萜类物质合成中的功能分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
808-816
栏目:
园艺
出版日期:
2026-04-30

文章信息/Info

Title:
Functional analysis of linalool synthase gene (LIS) in terpenoid synthesis of lavender
作者:
张明浩; 王爱凡; 龚林涛; 苏秀娟
(新疆农业大学农学院/新疆作物生物育种重点实验室/新疆农业大学薰衣草研究所,新疆乌鲁木齐830052)
Author(s):
ZHANG Minghao; WANG Aifan; GONG Lintao; SU Xiujuan
(College of Agronomy, Xinjiang Agricultural University/Xinjiang Key Laboratory of Crop Biological Breeding/Lavender Research Institute, Xinjiang Agricultural University, Urumqi 830052, China)
关键词:
芳樟醇合酶基因（LIS）; 薰衣草; 萜类物质
Keywords:
linalool synthase gene (LIS); lavender; terpenoids
分类号:
TQ654
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.017
文献标志码:
A
摘要:
为探究芳樟醇合酶基因(LIS)在薰衣草萜类物质代谢调控中的功能,本研究采用农杆菌介导法对高产精油品种杂薰衣草(Lavandula×intermedia)叶片进行遗传转化,获得过表达LiLIS基因薰衣草植株；通过分析其萜类合成途径中相关基因的表达量以及萜类化合物含量的变化,验证LiLIS1和LiLIS2基因在薰衣草中的功能。结果表明,与野生型(WT)薰衣草相比,过表达LiLIS1和LiLIS2基因均能显著提升薰衣草中单萜芳樟醇和薰衣草醇的含量（P<0.05）,同时过表达LiLIS1和LiLIS2基因植株中类胡萝卜素含量不同程度提升；而单萜乙酸薰衣草酯含量不同程度下降，过表达LiLIS2基因植株中樟脑含量显著下降（P<0.05）。因此,LiLIS基因在薰衣草萜类物质代谢合成中发挥重要作用,本研究结果可以为薰衣草品种改良提供理论基础及基因资源。
Abstract:
To investigate the function of the linalool synthase gene (LIS) in the regulation of terpenoid metabolism in lavender, Agrobacterium-mediated genetic transformation was used to modify the leaves of lavender (Lavandula×intermedia), generating lavender plants overexpressing the LiLIS gene. By analyzing the expression levels of genes involved in the terpenoid biosynthesis pathway and changes in terpenoid compound content, the functions of the LiLIS1 and LiLIS2 genes in lavender were verified. The results showed that, compared with wild-type (WT) lavender, overexpression of the LiLIS1 and LiLIS2 genes significantly increased the content of the monoterpenes linalool and lavandulol in lavender （P<0.05）. Additionally, carotenoid content in plants overexpressing LiLIS1 and LiLIS2 was increased to varying degrees. However, the content of monoterpene lavandulyl acetate decreased to varying degrees. The content of camphor decreased significantly in plants overexpressing the LiLIS2 gene （P<0.05）. Therefore, the LiLIS gene plays an important role in the metabolism and biosynthesis of terpenoids in lavender. These findings can provide a theoretical basis and genetic resources for the improvement of lavender cultivars.

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备注/Memo

备注/Memo:
收稿日期：2025-05-06基金项目：新疆维吾尔自治区重大科技专项(2022A03004-1)；国家自然科学基金项目(31760429)作者简介：张明浩(1999-),男,新疆乌鲁木齐人,硕士研究生,主要从事薰衣草分子育种研究。(E-mail)827432607@qq.com通讯作者：苏秀娟,(E-mail)smm1980@yeah.net
更新日期/Last Update: 2026-05-11