[1]杨培华,张弛,马佳伟,等.生姜HSP70家族基因鉴定及其响应高温胁迫的表达分析[J].江苏农业学报,2026,42(04):770-785.[doi:doi:10.3969/j.issn.1000-4440.2026.04.014]
　YANG Peihua,ZHANG Chi,MA Jiawei,et al.Identification of HSP70 family genes in ginger and their expression analysis in response to high temperature stress[J].,2026,42(04):770-785.[doi:doi:10.3969/j.issn.1000-4440.2026.04.014]
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生姜HSP70家族基因鉴定及其响应高温胁迫的表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
770-785
栏目:
园艺
出版日期:
2026-04-30

文章信息/Info

Title:
Identification of HSP70 family genes in ginger and their expression analysis in response to high temperature stress
作者:
杨培华1; 张弛1; 马佳伟1; 刘佳颖1; 万盼1; 吴子昱1; 梁红艳2; 叶亮3; 朱永兴1
(1.长江大学园艺园林学院/香辛园艺植物种质创新与利用湖北省重点实验室,湖北荆州434025；2.荆州市农业科学院,湖北荆州434025；3.宜昌犁沣农业科技有限责任公司,湖北宜昌443113)
Author(s):
YANG Peihua1; ZHANG Chi1; MA Jiawei1; LIU Jiaying1; WAN Pan1; WU Ziyu1; LIANG Hongyan2; YE Liang3; ZHU Yongxing1
(1.College of Horticulture and Gardening, Yangtze University/Hubei Key Laboratory of Spices & Horticultural Plant Germplasm Innovation & Utilization, Jingzhou 434025, China;2.Jingzhou Academy of Agricultural Sciences, Jingzhou 434025, China;3.Yichang Lifeng Agricultural Science and Technology Co., Ltd., Yichang 443113, China)
关键词:
生姜; HSP70家族基因; 高温胁迫; 表达分析
Keywords:
ginger;  HSP70 family genes; high temperature stress; expression analysis
分类号:
S632.5
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.014
文献标志码:
A
摘要:
为解析生姜(Zingiber officinale Roscoe)热激蛋白(Heat shock proteins,HSP)基因家族的信息,本研究基于生姜基因组数据,分析鉴定了ZoHSP70家族基因及其表达模式,并进一步利用实时荧光定量反转录聚合酶链式反应(qRT-PCR)检测了8个ZoHSP70基因在高温胁迫下的表达特性。研究结果表明,在生姜基因组中共鉴定到31个ZoHSP70家族基因；系统进化树显示,ZoHSP70家族基因可分为5个亚家族,位于同一亚家族的HSP70基因具有相似的基因结构。ZoHSP70家族基因编码的蛋白质氨基酸序列长度在163 aa至909 aa之间,相对分子量为18 120～101 610,除了ZoHSP70-26蛋白为碱性蛋白外,其余均为酸性蛋白。物种内共线性分析结果表明,ZoHSP70家族基因主要以片段复制为进化驱动力。顺式作用调控元件分析发现,ZoHSP70家族基因上游启动子区域内含有与光响应、生长发育响应、逆境胁迫响应、激素响应相关的作用元件。转录组测序数据分析结果显示,ZoHSP70家族基因有一定的组织表达特异性且响应干旱、低温等逆境胁迫,其中,ZoHSP70-10、ZoHSP70-24、ZoHSP70-30等基因在生姜不同生长时期、不同组织以及干旱、低温和淹水胁迫下均有较高表达。qRT-PCR分析结果表明,与0 h相比,ZoHSP70-24基因在高温胁迫处理4 h时表达量显著降低（P<0.05）,随着胁迫时间的延长,其基因表达量迅速升高,至24 h时表达量达到峰值；基因ZoHSP70-4、ZoHSP70-10、ZoHSP70-20在高温胁迫处理4 h时表达量最高,其余处理时间基因表达量均显著低于4 h（P<0.05）,但仍显著高于CK的表达量（P<0.05）。本研究结果为进一步解析生姜在不同非生物胁迫下HSP70家族基因参与调控的分子机制提供了理论参考。
Abstract:
To analyze the information of heat shock protein (HSP) gene family in Zingiber officinale Roscoe, the ZoHSP70 family genes and their expression patterns were analyzed and identified based on the ginger genome data, and the expression characteristics of eight ZoHSP70 genes under high temperature stress were further detected by quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). The results showed that 31 ZoHSP70 family genes were identified in the ginger genome. The phylogenetic tree showed that ZoHSP70 family genes could be divided into five subfamilies, and HSP70 genes in the same subfamily exhibited similar gene structures. The amino acid sequence lengths of the proteins encoded by the ZoHSP70 family genes ranged from 163 aa to 909 aa， with relative molecular weights ranging from 18 120 to 101 610. ZoHSP70-26 is an alkaline protein, while the remaining proteins are acidic. Intra-species colinearity analysis showed that the ZoHSP70 family genes were mainly driven by fragment replication. The analysis of cis-acting regulatory elements showed that the upstream promoter regions of ZoHSP70 family genes contained elements related to light, growth and development response, stress response and hormone response. Transcriptome sequencing data analysis showed that ZoHSP70 family genes had certain tissue expression specificity and responded to stresses such as drought and low temperature. Among them, genes such as ZoHSP70-10, ZoHSP70-24, and ZoHSP70-30 showed higher expression levels across different growth stages, various tissues, and under drought, low temperature and waterlogging stresses. The results of qRT-PCR analysis showed that compared with 0 h, the expression of ZoHSP70-24 gene was significantly decreased at 4 h under high temperature stress (P<0.05). With the prolongation of stress time, the expression of ZoHSP70-24 gene increased rapidly and reached the peak at 24 h. The expression levels of genes ZoHSP70-4, ZoHSP70-10, and ZoHSP70-20 were the highest at 4 h of high temperature stress treatment, and the gene expression levels at other treatment time points were significantly lower than those at 4 h (P<0.05), but still significantly higher than CK (P<0.05). In summary, this study provides a theoretical reference for further analysis of the molecular mechanisms by which HSP70 family genes participate in the regulation of ginger under different abiotic stresses.

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备注/Memo

备注/Memo:
收稿日期：2025-07-25基金项目：湖北省国际科技合作项目(2024EHA011)；长江大学湿地生态与农业利用教育部工程研究中心开放基金项目(KFG202422)作者简介：杨培华(2001-),女,湖北恩施人,硕士研究生,主要从事蔬菜逆境生理研究。(E-mail)yph919701@163.com通讯作者：朱永兴,(E-mail)xbnlzyx@163.com
更新日期/Last Update: 2026-05-11