[1]陈春娜,李正一,徐飞,等.厚颌鲂幼鱼对急性高温胁迫的生理适应机制[J].江苏农业学报,2026,42(04):763-769.[doi:doi:10.3969/j.issn.1000-4440.2026.04.013]
　CHEN Chunna,LI Zhengyi,XU Fei,et al.Physiological adaptation mechanism of juvenile Megalobrama pellegrini to acute high temperature stress[J].,2026,42(04):763-769.[doi:doi:10.3969/j.issn.1000-4440.2026.04.013]
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厚颌鲂幼鱼对急性高温胁迫的生理适应机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
763-769
栏目:
畜牧兽医·水产养殖
出版日期:
2026-04-30

文章信息/Info

Title:
Physiological adaptation mechanism of juvenile Megalobrama pellegrini to acute high temperature stress
作者:
陈春娜; 李正一; 徐飞; 刘红; 曾皓宇; 黄颖颖
(四川省农业科学院水产研究所/四川省水产研究所/长江上游鱼类资源保护与利用四川省重点实验室,四川成都611731)
Author(s):
CHEN Chunna; LI Zhengyi; XU Fei; LIU Hong; ZENG Haoyu; HUANG Yingying
(Fisheries Research Institute, Sichuan Academy of Agricultural Sciences/Sichuan Fisheries Research Institute/Fishes Conservation and Utilization in the Upper Reaches of the Yangtze River Key Laboratory of Sichuan Province, Chengdu 611731, China)
关键词:
厚颌鲂; 高温胁迫; 肝脏; 抗氧化酶; 热休克蛋白; 组织结构
Keywords:
Megalobrama pellegrini; heat stress; liver; antioxidant enzymes; heat shock protein; tissue structure
分类号:
S965.119
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.013
文献标志码:
A
摘要:
为探究急性高温胁迫对厚颌鲂(Megalobrama pellegrini)幼鱼的生理影响,本研究选用池塘培育的1龄健康厚颌鲂幼鱼作为试验对象,驯养2周后,设置18 ℃常温对照(CK)和32 ℃高温胁迫2个处理,于不同温度处理后0 h、6 h、12 h、24 h、48 h和96 h进行取样,测定厚颌鲂幼鱼肝脏超氧化物歧化酶(SOD)活性和过氧化氢酶(CAT)活性、热休克基因HSP60α和HSP70α的表达水平,并进行肝组织病理学观察,解析厚颌鲂幼鱼对急性高温胁迫的生理响应特征。结果表明,随着急性高温胁迫持续时间的增加,厚颌鲂幼鱼肝脏SOD、CAT活性呈先升高后降低的变化趋势,均于胁迫后24 h达到峰值。常温对照下,厚颌鲂幼鱼肝脏HSP70α和HSP60α基因表达水平无显著变化,而高温处理12 h和24 h HSP70α基因表达水平显著上调,高温处理6 h和24 h HSP60α基因表达水平亦显著高于CK。随着高温胁迫时间延长,厚颌鲂幼鱼肝脏组织出现大面积肝细胞空泡化和肝窦扩张等病理特征。综上,急性高温胁迫会导致厚颌鲂幼鱼产生应激损伤,引起肝组织病理学变化,厚颌鲂幼鱼通过快速激活热休克蛋白系统和抗氧化酶防御体系来应对胁迫。
Abstract:
To explore the physiological effects of acute high temperature stress on juvenile Megalobrama pellegrini, one-year-old healthy juvenile Megalobrama pellegrini cultured in ponds were selected as experimental subjects. After two weeks of domestication, two temperature treatment groups of 18 ℃ normal temperature control (CK) and 32 ℃ high temperature stress were set up. After different temperature treatments, samples were taken at 0 h, 6 h, 12 h, 24 h, 48 h and 96 h, respectively. The activities of superoxide dismutase (SOD) and catalase (CAT), the gene expression levels of heat shock genes HSP60α and HSP70α in the liver of juvenile Megalobrama pellegrini were determined, and the physiological response characteristics of juvenile Megalobrama pellegrini to acute high temperature stress were analyzed by combining with histopathological observation of liver. The results showed that with the increase of the duration of acute high temperature stress, the activities of SOD and CAT in the liver of juvenile Megalobrama pellegrini increased first and then decreased, reaching the peak at 24 h after stress. Under normal temperature control, there was no significant change in the expression levels of HSP70α and HSP60α genes in the liver of juvenile Megalobrama pellegrini, while the expression levels of HSP70α gene were significantly up-regulated at 12 h and 24 h after high temperature treatment, and the expression levels of HSP60α gene were also significantly higher than those of CK at 6 h and 24 h after high temperature treatment. With the prolongation of high temperature stress time, the liver tissue of juvenile Megalobrama pellegrini showed pathological characteristics such as large area of hepatocyte vacuolization and hepatic sinus dilatation. In summary, acute high temperature stress can cause stress damage in juvenile Megalobrama pellegrini and cause pathological changes in liver tissue. Juvenile Megalobrama pellegrini responds to stress by rapidly activating the heat shock protein system and antioxidant enzyme defense system.

参考文献/References:

[1]闫辰宇,姚世博. 全球和中国气候变化特征与趋势解析[N]. 中国气象报,2025-06-30(3).
[2]王盛辉. 长江干流水温长时序重构及变化特征[J]. 长江科学院院报,2025,42(10):54-63.
[3]靳纪明,路晶晶,李小勇,等. 鱼类对高温胁迫响应及机制研究进展[J]. 水产科学,2025,44(1):151-161.
[4]丰超杰,张颖,张永泉,等. 急性高温胁迫对黑龙江茴鱼血清生化指标、消化酶、抗氧化酶活性及其基因表达的影响[J]. 淡水渔业,2023,53(6):37-45.
[5]王润,刘洋,杨英明,等. 半滑舌鳎响应急性高温胁迫的生理变化和相关基因表达[J]. 渔业科学进展,2024,45(4):24-33.
[6]董福霖,黄天晴,刘恩慧,等. 高温胁迫对虹鳟(Oncorhynchus mykiss)肝脏中抗氧化酶活性和免疫相关基因表达的影响[J]. 海洋与湖沼,2023,54(1):225-232.
[7]KIM J H, KIM S K, HUR Y B. Temperature-mediated changes in stress responses,acetylcholinesterase,and immune responses of juvenile olive flounder Paralichthys olivaceus in a bio-floc environment[J]. Aquaculture,2019,506:453-458.
[8]丰超杰,刘霞飞,张颖,等. 高温胁迫下黑龙江茴鱼幼鱼肝脏组织结构变化及转录组表达特征[J]. 大连海洋大学学报,2023,38(4):603-614.
[9]何雨,刘峰,张天乐,等. 高温胁迫对小黄鱼肝脏组织结构和细胞凋亡的影响[J]. 浙江农业学报,2024,36(1):58-66.
[10]MA F, ZHAO L, MA R L, et al. FoxO signaling and mitochondria-related apoptosis pathways mediate tsinling lenok trout (Brachymystax lenok tsinlingensis) liver injury under high temperature stress[J]. International Journal of Biological Macromolecules,2023,251:126404.
[11]金新萍,谢倩,吕斌,等. 金鱼HSC70和HSP40基因克隆及其原核表达[J]. 南方农业学报,2018,49(2):367-374.
[12]刘丽丽,王晓雯,朱建亚,等. 虎皮鱼热激蛋白PtHsp70基因序列与低温表达分析[J]. 水产科学,2019,38(6):774-782.
[13]辛苑茹,温海深,李吉方,等. 急性高温胁迫对虹鳟二倍体和三倍体幼鱼hsps基因表达的影响[J]. 中国海洋大学学报(自然科学版),2019,49(3):129-137.
[14]王晓雯,张蓉,朱建亚,等. 急性热应激对西伯利亚鲟肝功指标及肝脏热休克蛋白表达的影响[J]. 四川农业大学学报,2019,37(1):122-128.
[15]朱凌威,张朝阳,刘钊,等. 杂交黄颡鱼hsp70基因核心序列的克隆、表达及其在高温应激下的组织表达[J]. 渔业科学进展,2021,42(1):47-55.
[16]李文静,王剑伟,谢从新,等. 厚颌鲂的年龄结构及生长特性[J]. 中国水产科学,2007,14(2):215-222.
[17]张洁若. 养殖密度和投喂策略对厚颌鲂幼鱼生长、饲料利用和抗应激能力的影响[D]. 海口:海南大学,2020.
[18]刘军. 长江上游特有鱼类受威胁及优先保护顺序的定量分析[J]. 中国环境科学,2004,24(4):395-399.
[19]李文静,王剑伟,谢从新,等. 厚颌鲂(Megalobrama pellegrini)的繁殖生物学特征[J]. 生态学报,2007,27(5):1917-1925.
[20]杜长雷,赵娜,王永明,等. 厚颌鲂精子的显微及超微结构观察[J]. 西南师范大学学报(自然科学版),2012,37(2):72-76.
[21]杜长雷. 厚颌鲂卵巢发育与卵子发生的形态和化学组分变化研究[D]. 重庆:西南大学,2011.
[22]袁锡立. 厚颌鲂驯养及人工繁殖技术研究[J]. 中国水产,2013(3):55-56.
[23]王瑾瑾. 狭义鲌亚科及其相关类群系统发育关系研究及厚颌鲂遗传多样性分析[D]. 重庆:西南大学,2013.
[24]易少奎,张新辉,杨坤,等. 厚颌鲂核型分析与DNA含量的测定[J]. 华中农业大学学报,2014,33(1):80-84.
[25]曲焕韬,陈磊,廖建新,等. 投喂策略对厚颌鲂幼鱼的生长、肠道消化酶活性和形态学的影响[J]. 海南大学学报(自然科学版),2021,39(1):29-35.
[26]王尧,陈晨光,张洁若,等. 养殖密度对厚颌鲂幼鱼生长、饲料利用及肠道抗氧化应激性能的影响[J]. 渔业科学进展,2022,43(1):106-114.
[27]李芹,唐洪玉,郑永华,等. 禁食后再投喂对厚颌鲂幼鱼生化及非特异性免疫指标的影响[J]. 西北农林科技大学学报(自然科学版),2015,43(2):49-57.
[28]杨艳红,郑永华,王磊. 重金属(Hg2+、Pb2+、Cr6+)对厚颌鲂幼鱼单一急性毒性效应[J]. 科学养鱼,2017(5):57-58.
[29]张文静,胡文达,郑永华. 重金属铜、镉对厚颌鲂幼鱼的毒性作用[J]. 科学养鱼,2012(9):50-52.
[30]张晨光,丁炜东,曹哲明,等. 急性高温胁迫对翘嘴鳜幼鱼抗氧化酶和消化酶活性及热休克蛋白基因表达的影响[J]. 南方农业学报,2021,52(3):815-826.
[31]姜旭阳,黄铭,杨小刚,等. 急性高温胁迫对虹鳟和硬头鳟幼鱼抗氧化酶活性的影响[J]. 中国水产科学,2021,28(1):57-65.
[32]王国成. 高温胁迫对白梭吻鲈生理生化的影响及其HSC70基因的克隆与表达[D]. 苏州:苏州大学,2017.
[33]DAWOOD M A O, NORELDIN A E, SEWILAM H. Blood biochemical variables,antioxidative status,and histological features of intestinal,gill,and liver tissues of African catfish (Clarias gariepinus) exposed to high salinity and high-temperature stress[J]. Environmental Science and Pollution Research International,2022,29(37):56357-56369.
[34]杨健,陈刚,黄建盛,等. 温度和盐度对军曹鱼幼鱼生长与抗氧化酶活性的影响[J]. 广东海洋大学学报,2007,27(4):25-29.
[35]LUSHCHAK V I, BAGNYUKOVA T V. Temperature increase results in oxidative stress in goldfish tissues. 2. Antioxidant and associated enzymes[J]. Comparative Biochemistry and Physiology Part C:Toxicology & Pharmacology,2006,143(1):36-41.
[36]MARTNEZ-LVAREZ R M, MORALES A E, SANZ A. Antioxidant defenses in fish:biotic and abiotic factors[J]. Reviews in Fish Biology and Fisheries,2005,15(1):75-88.
[37]MADEIRA D, NARCISO L, CABRAL H N, et al. Influence of temperature in thermal and oxidative stress responses in estuarine fish[J]. Comparative Biochemistry and Physiology Part A:Molecular & Integrative Physiology,2013,166(2):237-243.
[38]GHISELLI A, SERAFINI M, NATELLA F, et al. Total antioxidant capacity as a tool to assess redox status:critical view and experimental data[J]. Free Radical Biology and Medicine,2000,29(11):1106-1114.
[39]YU B P. Cellular defenses against damage from reactive oxygen species[J]. Physiological Reviews,1994,74(1):139-162.
[40]BECHMANN L P, HANNIVOORT R A, GERKEN G, et al. The interaction of hepatic lipid and glucose metabolism in liver diseases[J]. Journal of Hepatology,2012,56(4):952-964.
[41]陆健,张佳佳,周国勤,等. 急性高温胁迫对大口黑鲈“优鲈3号”组织损伤及HSPs基因表达的影响[J]. 水产科学,2021,40(4):508-515.
[42]周彦静,刘哲,夏斌鹏,等. 持续热应激对虹鳟肝脏组织形态结构的影响[J]. 甘肃农业大学学报,2017,52(6):1-5.
[43]刘波. 高温应激与大黄蒽醌提取物对团头鲂生理反应及相关应激蛋白表达的影响[D]. 南京:南京农业大学,2012.
[44]CUI Y T, LIU B, XIE J, et al. The effect of hyperthermia on cell viability,oxidative damage,and heat shock protein expression in hepatic cells of grass carp (Ctenopharyngodon idellus)[J]. Journal of Thermal Biology,2013,38(6):355-361.
[45]杨鑫. 高温胁迫对花鲈生理和能量代谢的影响与机制初探[D]. 厦门:集美大学,2024.
[46]史琛榆,赵淳朴,胡艺潇,等. 银鲳应对高温胁迫的生理响应及其相关基因表达研究[J]. 应用海洋学学报,2022,41(1):1-7.
[47]ZHOU A G, XIE S L, WANG Z L, et al. HSP60 expression profile under different extreme temperature stress in albino northern snakehead,Channa argus[J]. Cell Stress & Chaperones,2018,23(4):791-796.
[48] HABTE-TSION M, REN M C, GE X P, et al. Increase in temperature affects the growth,immune,and antioxidant status and hepatopancreatic gene expressions of antioxidant enzymes and heat-shock proteins of blunt snout bream,Megalobrama amblycephala fry[J]. Science & Technology,2016,2(8):408-426.
[49]强俊,杨弘,王辉,等. 急性温度应激对吉富品系尼罗罗非鱼(Oreochromis niloticus)幼鱼生化指标和肝脏HSP70 mRNA表达的影响[J]. 海洋与湖沼,2012,43(5):943-953.
[50]YOST H J, LINDQUIST S. Heat shock proteins affect RNA processing during the heat shock response of Saccharomyces cerevisiae[J]. Molecular and Cellular Biology,1991,11(2):1062-1068.
[51]李佳. 温度和盐度对菲律宾蛤仔(Ruditapes philippinarum)热休克蛋白家族基因表达的影响[D]. 大连:大连海洋大学,2016.

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备注/Memo

备注/Memo:
收稿日期：2025-07-28基金项目：四川省科技计划项目(2023NSFSC0209)作者简介：陈春娜(1981-),女,四川自贡人,硕士,副研究员,研究方向为水产动物生理生化。(E-mail)hc_1981_2001@sina.com通讯作者：黄颖颖,(E-mail)hz-yli@163.com
更新日期/Last Update: 2026-05-11