[1]魏黎欣,李泽宇,赵敏,等.寡雄腐霉菌株GAQ1对黄瓜猝倒病的防效及对黄瓜的促生作用[J].江苏农业学报,2026,42(04):701-708.[doi:doi:10.3969/j.issn.1000-4440.2026.04.006]
　WEI Lixin,LI Zeyu,ZHAO Min,et al.Biocontrol efficacy of Pythium oligandrum strain GAQ1 against cucumber damping-off and its growth-promoting effects on cucumber[J].,2026,42(04):701-708.[doi:doi:10.3969/j.issn.1000-4440.2026.04.006]
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寡雄腐霉菌株GAQ1对黄瓜猝倒病的防效及对黄瓜的促生作用()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
701-708
栏目:
植物保护
出版日期:
2026-04-30

文章信息/Info

Title:
Biocontrol efficacy of Pythium oligandrum strain GAQ1 against cucumber damping-off and its growth-promoting effects on cucumber
作者:
魏黎欣1; 2; 李泽宇2; 3; 赵敏2; 苏东徽2; 周冬梅2; 魏利辉2; 乐秀虎1; 王楠2
(1.河北工程大学园林与生态工程学院,河北邯郸056038；2.江苏省农业科学院植物保护研究所,江苏南京210014；3.南京农业大学植物保护学院,江苏南京211800 )
Author(s):
WEI Lixin1; 2; LI Zeyu2; 3; ZHAO Min2; SU Donghui2; ZHOU Dongmei2; WEI Lihui2; LE Xiuhu1; WANG Nan2
(1.School of Landscape and Ecological Engineering, Hebei University of Engineering, Handan 056038, China;2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;3.College of Plant Protection, Nanjing Agricultural University, Nanjing 211800, China)
关键词:
寡雄腐霉; 瓜果腐霉; 黄瓜; 生物防治; 促生作用
Keywords:
Pythium oligandrum; Pythium aphanidermatum; Cucumis sativus; biological control; growth-promoting effect
分类号:
S436.421
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.006
文献标志码:
A
摘要:
生物防治因具有环境友好和安全性,在作物应对生物与非生物胁迫中备受关注,然而当前市场上的生物防治产品数量仍远不及化学农药。本研究以从生姜田块中分离获得的生防卵菌——寡雄腐霉(Pythium oligandrum)菌株GAQ1为研究对象,系统评估了其对瓜果腐霉(Pythium aphanidermatum)的拮抗作用，对黄瓜猝倒病的防治效果以及对黄瓜植株的促生作用。结果表明,寡雄腐霉菌株GAQ1能够通过菌丝缠绕导致瓜果腐霉菌丝大面积失活或死亡。盆栽试验结果显示,寡雄腐霉菌株GAQ1处理显著降低了黄瓜猝倒病的发病率,病情指数由71.9下降至21.9,防治效果达69.57%。此外,黄瓜幼苗经GAQ1发酵液处理28 d后,黄瓜植株的株高、根长、叶片数、SPAD值、地上部鲜重和地下部鲜重等生长指标均显著提高,增幅分别为9.72%、65.48%、18.67%、5.74%、24.19%和78.35%。综上所述,寡雄腐霉菌株GAQ1不仅对黄瓜猝倒病具有显著防治效果,还能明显促进植株生长。本研究结果为寡雄腐霉菌株GAQ1的开发与应用提供了理论数据,为瓜果腐霉所致病害的生物防治提供了新的候选菌株资源,对实现绿色农业可持续发展具有积极意义。
Abstract:
Biological control has garnered significant attention due to its environmental friendliness and safety in addressing both biotic and abiotic stresses in crops. However, the number of commercially available biological control products remains far lower than that of chemical pesticides. This study focused on the biocontrol oomycete Pythium oligandrum strain GAQ1 ginger isolated from ginger field soil, and systematically evaluated its antagonistic effects against P. aphanidermatum, its efficacy in controlling cucumber damping-off, and its growth-promoting effects on cucumber plants. The results demonstrated that GAQ1 could achieve mycoparasitism through hyphal coiling, leading to extensive inactivation or death of P. aphanidermatum hyphae. Pot experiments revealed that GAQ1 treatment significantly reduced the incidence of cucumber damping-off, with the disease index decreasing from 71.9 to 21.9 and a control efficacy of 69.57%. Furthermore, after four weeks of GAQ1 treatment, the growth parameters of cucumber plants including plant height, root length, leaf number, SPAD value, aboveground fresh weight, and underground fresh weight were significantly enhanced, with increases of 9.72%, 65.48%, 18.67%, 5.74%, 24.19%, and 78.35%, respectively. In conclusion, P. oligandrum GAQ1 not only exhibited remarkable control efficacy against cucumber damping-off but also significantly promoted plant growth. This study provides data support for the development and application of GAQ1, offers a new candidate strain resource for the biological control of diseases caused by P. aphanidermatum, and holds significant importance for advancing sustainable green agriculture.

参考文献/References:

[1]BENHAMOU N, LE FLOCH G, VALLANCE J, et al. Pythium oligandrum:an example of opportunistic success[J]. Microbiology,2012,158(11):2679-2694.
[2]姜一鸣,黄海鹰,陈勇. 寡雄腐霉生防机理及应用研究进展[J]. 中国生物防治学报,2017,33(3):401-407.
[3]BLONONKOV K, HYSKOV V, CHMELK J, et al. Pythium oligandrum in plant protection and growth promotion:secretion of hydrolytic enzymes,elicitors and tryptamine as auxin precursor[J]. Microbiological Research,2022,258:126976.
[4]XUE Y W, LI W S, LI M N, et al. Biological control of a root-knot nematode Meloidogyne incognita infection of tomato (Solanum lycopersicum L. ) by the oomycete biocontrol agent Pythium oligandrum[J]. Journal of Fungi,2024,10(4):265.
[5]薛原,王真娣,袁嘉雯,等. 寡雄腐霉生防机制及其在农业中的应用进展[J]. 生物灾害科学,2024,47(2):149-158.
[6]PICARD K, PONCHET M, BLEIN J P, et al. Oligandrin. A proteinaceous molecule produced by the mycoparasite Pythium oligandrum induces resistance to Phytophthora parasitica infection in tomato plants[J]. Plant Physiology,2000,124(1):379-395.
[7]王子洋,熊雨洁,冯发运,等. 寡雄腐霉对禾谷镰刀菌防效及其产孢诱导剂筛选[J]. 江苏农业科学,2023,51(18):101-107.
[8]耿明明,黄建国. 寡雄腐霉发酵液的动物毒性及对辣椒的促生防病效应[J]. 植物保护学报,2016,43(2):307-313.
[9]赵建,吴叶宽,袁玲,等. 寡雄腐霉发酵液对烤烟生长的影响及对烟草黑胫病的防治作用[J]. 植物保护学报,2013,40(1):68-72.
[10]SAYED S R M, ABDELMOHSEN S A M, ABDELZAHER H M A, et al. Myco-suppression analysis of soybean (Glycine max) damping-off caused by Pythium aphanidermatum[J]. Plants,2021,10(4):788.
[11]YOU X D, BARRAUD J, TOJO M. Suppressive effects of Pythium oligandrum on soybean damping off caused by P. aphanidermatum and P. myriotylum[J]. Annual Report of the Kansai Plant Protection Society,2019,61:9-13.
[12]李伟山,纠敏,周冬梅,等. 寡雄腐霉GAQ1对辣椒疫病的防效及对辣椒的促生作用[J]. 植物保护学报,2022,49(3):956-965.
[13]NG C A, PERNICA M, LITVANOVA K, et al. Biocontrol using Pythium oligandrum during malting of Fusarium-contaminated barley[J]. Fermentation,2023,9(3):257.
[14]PATKOWSK E. Effectiveness of grapefruit extract and Pythium oligandrum in the control of bean and peas pathogens[J]. Journal of Plant Protection Resource,2006,46(1):15-28.
[15]CHEN S Q, DALY P, ZHOU D M, et al. The use of mutant and engineered microbial agents for biological control of plant diseases caused by Pythium:achievements versus challenges[J]. Fungal Biology Reviews,2022,40:76-90.
[16]欧阳由男,夏陆欣,朱练峰,等. 寡雄腐霉制剂“多利维生”对水稻的促长与防病增产效果[J]. 中国稻米,2007,13(6):48-51.
[17]贾瑞莲,耿明明,袁玲. 寡雄腐霉发酵液对温室番茄生长及灰霉病的防治作用[J]. 植物保护学报,2015,42(5):827-833.
[18]VESTBERG M, KUKKONEN S, SAARI K, et al. Microbial ino-culation for improving the growth and health of micropropagated strawberry[J]. Applied Soil Ecology,2004,27(3):243-258.
[19]MUTHUKUMAR A, ESWARAN A, SANJEEVKUMAS K. Exploitation of Trichoderma species on the growth of Pythium aphanidermatum in chilli[J]. Brazilian Journal of Microbiology,2011,42(4):1598-1607.
[20]POSTMA J, BONANTS P J, VAN OS E A. Population dynamics of Pythium aphanidermatum in cucumber grown in closed systems[J]. Mededelingen,2001,66(2a):47-59.
[21]AL-SHEIKH H. Two pathogenic species of Pythium:P. aphanidermatum and P. diclinum from a wheat field[J]. Saudi Journal of Biological Sciences,2010,17(4):347-352.
[22]RAI M, ABD-ELSALAM K A, INGLE A P. Pythium[M]. Boca Raton:CRC Press,2020：77-91.
[23]AL-MAHMOOLI I H, FINCKH M R, VELAZHAHAN R, et al. Pythium and Globisporangium species associated with cucumber rhizosphere causing damping-off and their effects on cucumber seed decay in Oman[J]. Archives of Microbiology,2024,206(9):374.
[24]PARVEEN T, MEENA M, JAIN T, et al. Pythium aphanidermatum and its control measures[M]. Boca Raton:CRC Press,2020：299-313.
[25]KAUR M, SHARMA P. Recent advances in cucumber (Cucumis sativus L. )[J]. The Journal of Horticultural Science and Biotechnology,2022,97(1):3-23.
[26]FENG S J, ZHANG J P, MU Z H, et al. Recent progress on the molecular breeding of Cucumis sativus L. in China[J]. Theoretical and Applied Genetics,2020,133(5):1777-1790.
[27]AFANDI A, BORJIGIN C, HIENO A, et al. Highly clonal Pythium aphanidermatum population infecting greenhouse plants in Japan[J]. Archives of Phytopathology and Plant Protection,2023,56(16):1255-1266.
[28]KAWICHA P, PENGPROH R, THANYASIRIWAT T, et al. Bioproducts derived from Bacillus stercoris isolate B. PNR1 and Streptomyces sp. isolate S. PNR29 for enhanced plant growth and disease control in tomato[J]. New Zealand Journal of Crop and Horticultural Science,2025,53(5):1530-1546.
[29]THEPBANDIT W, NAWONG S, ATHINUWAT D. Potential of a microbial co-culture composed of Bacillus vallismortis TU-Orga21 and Bacillus subtilis TU-Orga1 to improve the efficacy of controlling Damping-off caused by Pythium aphanidermatum in kale[J]. Plant Pathology,2025,74(6):1527-1543.
[30]陈凯,隋丽娜,赵忠娟,等. 木霉共培养发酵对黄瓜枯萎病的防治效果[J]. 中国生物防治学报,2022,38(1):108-114.
[31]SOROUR A,ZOBAIR N,GHANEM K,et al. Rhamnolipid from Pseudomonas sp. as a green surfactant for enhanced phytoreme-diation[J]. Scientific Reports,2025,15(1):29780.
[32]DALY P, CHEN S Q, XUE T Q, et al. Dual-transcriptomic,microscopic,and biocontrol analyses of the interaction between the bioeffector Pythium oligandrum and the Pythium soft-rot of ginger pathogen Pythium myriotylum[J]. Frontiers in Microbiology,2021,12:765872.
[33]PU X M, XIE B Y, LI P Q, et al. Analysis of the defence-related mechanism in cucumber seedlings in relation to root colonization by nonpathogenic Fusarium oxysporum CS-20[J]. FEMS Microbiology Letters,2014,355(2):142-151.
[34]PLAATSNITERINK A J V D .Monograph of the genus Pythium[J]. Studies in Mycology,1981,21:1-244.

备注/Memo

备注/Memo:
收稿日期:2025-09-09基金项目:国家特色蔬菜产业技术体系项目(CARS-24-C-01)；邯郸市科学技术研究与发展计划项目(21422012329)作者简介:魏黎欣(1999-),女,河南鹤壁人,硕士研究生,主要从事生防卵菌GAQ1的功能研究。(E-mail)986932891@qq.com通讯作者:乐秀虎,(E-mail)lexiuhu@163.com；王楠,(E-mail)20230035@jaas.ac.cn
更新日期/Last Update: 2026-05-11