[1]邓召,崔妮妮,余育林,等.超甜玉米籽粒维生素C含量关联位点定位及基因挖掘[J].江苏农业学报,2026,42(04):682-690.[doi:doi:10.3969/j.issn.1000-4440.2026.04.004]
　DENG Zhao,CUI Nini,YU Yulin,et al.Association mapping and gene mining for vitamin C content in super-sweet corn kernels[J].,2026,42(04):682-690.[doi:doi:10.3969/j.issn.1000-4440.2026.04.004]
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超甜玉米籽粒维生素C含量关联位点定位及基因挖掘()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年04期
页码:
682-690
栏目:
遗传育种·生理生化
出版日期:
2026-04-30

文章信息/Info

Title:
Association mapping and gene mining for vitamin C content in super-sweet corn kernels
作者:
邓召; 崔妮妮; 余育林; 文伟祺; 程昕昕; 余海兵
(安徽科技学院农学院,安徽凤阳233100)
Author(s):
DENG Zhao; CUI Nini; YU Yulin; WEN Weiqi; CHENG Xinxin; YU Haibing
(College of Agriculture, Anhui Science and Technology University, Fengyang 233100, China)
关键词:
超甜玉米; 维生素C; 全基因组关联分析; 候选基因
Keywords:
super-sweet corn; vitamin C; genome-wide association study; candidate genes
分类号:
S513
DOI:
doi:10.3969/j.issn.1000-4440.2026.04.004
文献标志码:
A
摘要:
为揭示超甜玉米籽粒营养物质累积的调控机制,本研究采用 BLINK 模型定位了调控超甜玉米籽粒维生素C含量的主效位点,并对候选基因进行了预测。结果表明,供试的200份超甜玉米微核心种质籽粒中维生素C含量为0.156～1.289 mg/g,均值为0.749 mg/g,呈正态分布；全基因组关联分析(GWAS)共关联到6个与籽粒维生素C含量显著相关的单核苷酸多态性(SNP)位点,表型变异解释率为9.53%～12.83%,其中表型变异率最高的是Affx-91328273位点；在显著SNP位点上下游200 kb的置信区间内共找到153个候选基因,其中已注释基因58个,占比为37.9%。鉴定出的GRMZM2G093098、GRMZM2G106061、GRMZM2G179308、GRMZM2G101274、GRMZM2G004222、GRMZM2G133631 6个基因是调控超甜玉米籽粒维生素C含量的关键基因,其主要参与细胞分裂、信号转导和能量代谢等途径,为深入理解超甜玉米籽粒维生素C含量的遗传机制提供了重要支撑。
Abstract:
To uncover the regulatory mechanisms underlying the accumulation of nutrients in the kernels of super-sweet corn, this study employed the BLINK model to identify the major loci regulating vitamin C content in super-sweet corn kernels and predict the candidate genes. The results showed that the vitamin C content in super-sweet corn kernels ranged from 0.156 mg/g to 1.289 mg/g, with an average of 0.749 mg/g, exhibiting a normal distribution. A genome-wide association study (GWAS) identified six single nucleotide polymorphism (SNP) loci significantly associated with kernel vitamin C content, with the phenotypic variation explained (PVE) ranging from 9.53% to 12.83%, among which the Affx-91328273 locus had the highest PVE. A total of 153 candidate genes were identified within the 200 kb confidence interval upstream and downstream of the significant SNP loci, of which 58 were annotated genes, accounting for 37.9%. Six genes including GRMZM2G093098, GRMZM2G106061, GRMZM2G179308, GRMZM2G101274, GRMZM2G004222 and GRMZM2G133631 were identifed as key genes regulating kernel vitamin C content in super-sweet corn. These genes were mainly involved in cell division, signal transduction, energy metabolism and other pathways. These findings provide important support for an in-depth understanding of the genetic mechanisms underlying kernel vitamin C content in super-sweet corn.

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备注/Memo

备注/Memo:
收稿日期：2025-05-30基金项目：安徽省高校自然科学研究重大项目(KJ2019ZD57)；安徽科技学院科研发展基金项目(FZ230126)；安徽省功能农业与功能食品重点实验室(安徽科技学院)项目(iFAST-2024-3)；安徽科技学院作物学高峰学科基金项目(XK-XJGF001)作者简介：邓召(2005-),男,安徽定远人,硕士研究生,主要从事玉米遗传育种研究。(E-mail)19505505830@163.com通讯作者：程昕昕,(E-mail)chengxin0901@163.com
更新日期/Last Update: 2026-05-11