[1]彭俊祥,李思鹏,刘杨,等.海南龙血树NBS-LRR基因家族的鉴定、密码子偏好性与表达分析[J].江苏农业学报,2026,42(03):625-637.[doi:doi:10.3969/j.issn.1000-4440.2026.03.021]
　PENG Junxiang,LI Sipeng,LIU Yang,et al.Identification,codon usage bias and expression analysis of NBS-LRR gene family in Dracaena cambodiana Pierre ex Gagn.[J].,2026,42(03):625-637.[doi:doi:10.3969/j.issn.1000-4440.2026.03.021]
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海南龙血树NBS-LRR基因家族的鉴定、密码子偏好性与表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年03期
页码:
625-637
栏目:
园艺
出版日期:
2026-03-31

文章信息/Info

Title:
Identification,codon usage bias and expression analysis of NBS-LRR gene family in Dracaena cambodiana Pierre ex Gagn.
作者:
彭俊祥1; 李思鹏2; 刘杨1; 徐艳红1; 魏建和1; 2
(1.中国医学科学院/北京协和医学院药用植物研究所/天然药物活性物质与功能国家重点实验室/濒危药材繁育国家工程实验室,北京100193；2.中国医学科学院/北京协和医学院药用植物研究所海南分所/海南省南药资源保护与开发重点实验室/国家中医药管理局沉香可持续利用重点研究室,海南海口570311)
Author(s):
PENG Junxiang1; LI Sipeng2; LIU Yang1; XU Yanhong1; WEI Jianhe1; 2
(1.Chinese Academy of Medical Sciences/Institute of Medicinal Plant Development, Peking Union Medical College/State Key Laboratory of Bioactive Substance and Function of Natural Medicines/National Enginee-ring Laboratory for Breeding of Endangered Medicinal Materials, Beijing 100193, China；2.Chinese Academy of Medical Sciences/Hainan Branch of the Institute of Medicinal Plant Development, Peking Union Medical College/Hainan Provincial Key Laboratory of Resources Conservation and Development of Southern Medicine/Key Laboratory of State Administration of Traditional Chinese Medicine for Agarwood Sustainable Utilization, Haikou 570311, China)
关键词:
海南龙血树; NBS-LRR; 基因家族分析; 密码子偏好性; 表达模式
Keywords:
Dracaena cambodiana Pierre ex Gagn.; NBS-LRR; gene family analysis; codon bias; pattern of expression
分类号:
S567.1+9;S687.9
DOI:
doi:10.3969/j.issn.1000-4440.2026.03.021
文献标志码:
A
摘要:
NBS-LRR是植物中最大的一个抗病基因家族,在植物抗性及免疫反应中发挥重要作用。本研究基于课题组完成的海南龙血树全基因组数据,利用生物信息学方法对海南龙血树NBS-LRR基因家族进行系统鉴定,并对其蛋白质理化性质、染色体定位、基因结构及密码子偏好性等进行分析。结果表明,海南龙血树中共有95个DcNBS-LRR编码基因,不均衡地分布在20条染色体中的14条上。这些基因分为CNL、CN、RNL、RN、NL和N 6个亚家族。海南龙血树NBS-LRR蛋白普遍具有疏水性,预测在细胞核、细胞质、细胞膜和叶绿体中都有分布。DcNBS-LRR蛋白具有10个相对保守的基序（Motif）,并且多数排列顺序稳定。密码子偏好性分析结果显示,DcNBS-LRR偏好以A/U结尾的密码子,高频密码子有31个,其中17个被认为是最优密码子；对称规则二图（PR2-plot）分析、有效密码子数（ENC）-plot分析以及中性绘图分析结果显示,DcNBS-LRR基因家族密码子使用偏好主要受自然选择的影响。密码子使用频率分析结果表明,酿酒酵母、烟草和拟南芥均可作为DcNBS-LRR基因异源表达的理想受体。此外,对DcNBS-LRR基因响应尖孢镰刀菌侵染的表达动态进行了分析,结果表明,大部分DcNBS-LRR基因在侵染前期和中期(接种后12 h、1 d、3 d、7 d)表达受到抑制,而在未侵染和侵染30 d时表达量较高。本研究是首次对海南龙血树中的NBS-LRR基因家族进行鉴定和特征分析,本研究结果将为揭示海南龙血树抗病机制以及龙血竭形成的分子机制研究提供线索,可为后续深入解析DcNBS-LRR的功能奠定基础。
Abstract:
NBS-LRR is the largest gene family among plant disease resistance genes and plays an important role in plant resistance and immune response. Based on the previously completed whole-genome data of Dracaena cambodiana Pierre ex Gagn. by our research group, this study systematically identified the NBS-LRR gene family of D. cambodiana using bioinformatics methods, and analyzed its physicochemical properties, chromosome localization, gene structure and codon preference. The results indicated that there were a total of 95 DcNBS-LRR coding genes in D. cambodiana, which were unevenly distributed on 14 out of 20 chromosomes. These genes were divided into six subfamilies including CNL, CN, RNL, RN, NL, and N. The NBS-LRR protein of D. cambodiana was generally hydrophobic and was predicted to be distributed in the nucleus, cytoplasm, cell membrane and chloroplast. DcNBS-LRR proteins had ten relatively conserved motifs, and most of them were arranged in stable order. Codon bias analysis showed that DcNBS-LRR preferred codons ending in A/U, and there were 31 high frequency codons, of which 17 were considered to be optimal codons. Parity rule 2 (PR2)-plot analysis, effective number of codon (ENC)-plot analysis and neutral plot analysis showed that the codon usage preference of DcNBS-LRR gene family was mainly influenced by natural selection. Codon usage frequency analysis indicated that Saccharomyces cerevisiae, Nicotiana tabacum and Arabidopsis thaliana could all serve as ideal receptors for heterologous expression of DcNBS-LRR genes. In addition, the expression dynamics of DcNBS-LRR genes in response to Fusarium oxysporum infection were analyzed. The results showed that the expression of most DcNBS-LRR genes was suppressed in the early stage and middle stage of infection (12 h, 1 d, 3 d, and 7 d), while the expression was higher in non-infection and 30 days of infection. This study is the first to identify and analyze the characteristics of the NBS-LRR gene family in D. cambodiana. The research results will provide clues for revealing the disease resistance mechanism of D. cambodiana and the molecular mechanism of Dragon’s blood formation, and lay the foundation for the subsequent in-depth analysis of the function of DcNBS-LRR.

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备注/Memo

备注/Memo:
收稿日期：2025-04-21基金项目：国家自然科学基金项目(82173925);海南省自然科学基金项目(322QN337)；中国医学科学院医学与健康科技创新工程项目(2021-I2M-1-032)；海南省优秀人才团队建设项目(HNYT20240003)作者简介：彭俊祥(2000-),男,内蒙古赤峰人,硕士研究生,主要从事植物与真菌互作机制研究。(Tel)15326832672;(E-mail)2824518687@qq.com通讯作者：徐艳红,(Tel)13269675108,(E-mail)xuyanhong99@163.com;魏建和, (Tel)01057833016,(E-mail)wjianh@263.net
更新日期/Last Update: 2026-04-17