[1]伏晓科,何文,钟玉蝶,等.树冠覆膜与双层套袋对不知火柑橘果实品质的影响[J].江苏农业学报,2026,42(02):399-406.[doi:doi:10.3969/j.issn.1000-4440.2026.02.019]
　FU Xiaoke,HE Wen,ZHONG Yudie,et al.Effects of canopy film-covering and double-layer bagging on fruit quality of Citrus reticulata cv. ‘Buzhihuo’[J].,2026,42(02):399-406.[doi:doi:10.3969/j.issn.1000-4440.2026.02.019]
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树冠覆膜与双层套袋对不知火柑橘果实品质的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
399-406
栏目:
园艺
出版日期:
2026-02-28

文章信息/Info

Title:
Effects of canopy film-covering and double-layer bagging on fruit quality of Citrus reticulata cv. ‘Buzhihuo’
作者:
伏晓科1; 何文2; 钟玉蝶3; 向娟1; 杨俊1
(1.眉山职业技术学院,四川眉山620020；2.四川农业大学,四川成都611130；3.成都市农业技术推广总站,四川成都610041)
Author(s):
FU Xiaoke1; HE Wen2; ZHONG Yudie3; XIANG Juan1; YANG Jun1
(1.Meishan Vocational and Technical College, Meishan 620020, China;2.Sichuan Agricultural University, Chengdu 611130, China;3.Chengdu Agricultural Technology Promotion General Station, Chengdu 610041, China)
关键词:
套袋; 树冠覆膜; 果实品质; 转录组; 差异表达基因
Keywords:
bagging; canopy film-covering; fruit quality; transcriptome; differentially expressed gene
分类号:
S666.9
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.019
文献标志码:
A
摘要:
探究单层套袋加树冠覆膜(SB+PF)处理和双层套袋(DB)处理2种不同防御低温冷害的处理对晚熟柑橘不知火(Citrus reticulata ‘Buzhihuo’)果实品质的影响及分子机制。结果表明,与SB+PF处理相比,DB处理极显著提高了单果重,显著降低了果皮厚度，极显著提高了果实横径和纵径。SB+PF处理果实果皮明度(L*)显著高于DB处理,但2种处理间叶绿素和类胡萝卜素含量差异不显著。与SB+PF处理相比,DB处理果实的可滴定酸含量显著提高，维生素C含量极显著提高。转录组分析发现,DB处理下有133个差异表达基因,其中85个下调,48个上调,这些基因富集在黄酮生物合成、α-亚麻酸代谢等通路。加权基因共表达网络分析(WGCNA)结果揭示了10个与糖酸代谢相关的转录因子。本研究结果表明，双层套袋通过调控微环境和相关基因表达改善了果实外观品质。
Abstract:
This study compared single-layer bagging plus canopy film-covering (SB+PF) treatment and double-layer bagging (DB) treatment to investigate their effects on fruit quality and molecular mechanisms for cold injury prevention in Citrus reticulata cv. ‘Buzhihuo’. The results showed that， (1) compared with SB+PF treatment, DB treatment extremely significantly increased single-fruit weight and significantly reduced peel thickness, while extremely significantly increased fruit transverse and longitudinal diameters. SB+PF treatment resulted in significantly higher peel brightness (L* value) compared with DB treatment, but no significant differences were observed in chlorophyll content or carotenoid content. (2) Compared with SB+PF treatment, DB treatment increased fruit titratable acid content significantly, and increased vitamin C content extremely significantly. (3) Transcriptome analysis revealed a total of 133 differentially expressed genes (DEGs) under DB treatment conditions, among which 85 were down-regulated genes and 48 were up-regulated genes. These genes showed enrichment in pathways such as flavonoid biosynthesis and α-linolenic acid metabolism. Through Weighted Gene Co-expression Network Analysis (WGCNA), ten transcription factors related to sugar-acid metabolism were screened out. The study demonstrates that double-layer bagging improves fruit appearance quality by regulating the micro-environment and gene expression.

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备注/Memo

备注/Memo:
收稿日期：2025-10-14基金项目：四川省科普培训项目(科普作品创作类)(2025JDKP0118)；眉山市指导性科技计划项目(2023KJZD083)作者简介：伏晓科(1993-),男,甘肃秦安人,硕士,讲师,主要从事果树栽培与采后生理研究。(E-mail)1175912581@qq.com通讯作者：杨俊, (E-mail)scmsyangjun@qq.com
更新日期/Last Update: 2026-03-16