[1]张晨,刘畅,冯国军,等.外源尿囊素对碱胁迫下菜豆种子萌发及抗性的影响[J].江苏农业学报,2026,42(02):392-398.[doi:doi:10.3969/j.issn.1000-4440.2026.02.018]
　ZHANG Chen,LIU Chang,FENG Guojun,et al.Effect of exogenous allantoin on common bean seed germination and resistance under alkali stress[J].,2026,42(02):392-398.[doi:doi:10.3969/j.issn.1000-4440.2026.02.018]
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外源尿囊素对碱胁迫下菜豆种子萌发及抗性的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
392-398
栏目:
园艺
出版日期:
2026-02-28

文章信息/Info

Title:
Effect of exogenous allantoin on common bean seed germination and resistance under alkali stress
作者:
张晨; 刘畅; 冯国军; 刘大军; 闫志山; 张泰峰; 杨晓旭
(黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150080)
Author(s):
ZHANG Chen; LIU Chang; FENG Guojun; LIU Dajun; YAN Zhishan; ZHANG Taifeng; YANG Xiaoxu
(College of Modern Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China)
关键词:
菜豆; 种子萌发; 碱胁迫; 尿囊素
Keywords:
common bean; seed germination; alkali stress; allantoin
分类号:
S643.1
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.018
文献标志码:
A
摘要:
为探究尿囊素对碱胁迫环境下菜豆种子萌发及生理特征的影响,本研究以碱敏感菜豆品种金冠为试验材料,在筛选敏感碱胁迫浓度和适宜尿囊素浸种浓度的基础上,设置蒸馏水浸种+蒸馏水萌发培养对照(CK)、蒸馏水浸种+40 mmol/L NaHCO3萌发培养处理(S)、2 mmol/L尿囊素浸种＋40 mmol/L NaHCO3萌发培养处理(S+All),测定培养后12 h、24 h、36 h、48 h、60 h和72 h菜豆种子中可溶性蛋白质、可溶性糖、脯氨酸等渗透调节物质的含量，菜豆子叶中超氧化物歧化酶(SOD)、过氧化物酶(POD)及过氧化氢酶(CAT)等抗氧化酶的活性及超氧阴离子自由基(O·-2)产生速率、丙二醛(MDA)含量。结果表明,2 mmol/L尿囊素浸种缓解了菜豆种子萌发的碱胁迫，发芽势、发芽率分别较蒸馏水处理提高了17.71%、17.76%。培养后12～72 h S处理菜豆种子的可溶性蛋白质含量、可溶性糖含量、脯氨酸含量及菜豆子叶中的O2·-产生速率、MDA含量总体高于CK,而菜豆子叶的抗氧化酶活性总体低于CK。S+All处理菜豆种子的可溶性蛋白质含量、可溶性糖含量、脯氨酸含量及菜豆子叶的抗氧化酶活性总体高于S处理,而菜豆子叶的O2·-产生速率和MDA含量总体低于S处理。本研究结果为尿囊素在碱胁迫环境下菜豆种植中的应用提供了依据。
Abstract:
To explore the effects of allantoin on seed germination and physiological characteristics of common bean under alkali stress, the alkali-sensitive common bean variety Jinguan was used as the experimental material in this study. Based on the screening of sensitive alkali stress concentration and suitable allantoin soaking concentration, distilled water soaking + distilled water germination culture control (CK), distilled water soaking + 40 mmol/L NaHCO3 germination culture treatment (S), 2 mmol/L allantoin soaking + 40 mmol/L NaHCO3 germination culture treatment (S+All ) were set. The contents of soluble protein, soluble sugar, proline and other osmotic adjustment substances in common bean seeds, the activities of antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), the production rate of superoxide anion free radical (O2·-) and the content of malondialdehyde (MDA) in cotyledons of common bean seeds were measured at 12 h, 24 h, 36 h, 48 h, 60 h and 72 h after culture. The results showed that the germination potential and germination rate of seeds treated with 2 mmol/L allantoin were 17.71% and 17.76% higher than those of distilled water treatment, respectively, which alleviated alkaline stress during the germination of common bean seeds. The soluble protein content, soluble sugar content, proline content in common bean seeds, and O2·- production rate and MDA content of the cotyledons of bean seeds treated with S at 12-72 h after culture were generally higher than those of CK, while the antioxidant enzyme activity was generally lower than that of CK in cotyledons of common bean. The soluble protein content, soluble sugar content, proline content in common bean seeds and antioxidant enzyme activity in cotyledons of common bean of S+All treatment were higher than those of S treatment, while the O·-2 production rate and MDA content in cotyledons of common bean were lower than those of S treatment. The results of this study provide a basis for the application of allantoin in bean planting under alkaline stress.

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备注/Memo

备注/Memo:
收稿日期：2025-04-14基金项目：黑龙江省自然科学基金优秀青年基金项目(YQ2024C046);黑龙江省“优秀青年教师基础研究支撑计划”项目(YQJH2024198)；黑龙江省高等学校基本科研业务费项目(2024-KYYWF-0110)作者简介：张晨(1999-),女,山东泰安人,硕士研究生,主要从事菜豆育种及种质资源创新研究。(E-mail)3086630848@qq.com通讯作者：杨晓旭,(E-mail)sunny19880106@126.com
更新日期/Last Update: 2026-03-16