[1]周扬达,郑建波,李飞,等.摄食不同饵料对翘嘴鳜脑组织、肝组织中基因表达的影响[J].江苏农业学报,2026,42(02):382-391.[doi:doi:10.3969/j.issn.1000-4440.2026.02.017]
　ZHOU Yangda,ZHENG Jianbo,LI Fei,et al.Effects of different feeding regimens on gene expression in brain and liver tissues of mandarin fish (Siniperca chuatisi)[J].,2026,42(02):382-391.[doi:doi:10.3969/j.issn.1000-4440.2026.02.017]
点击复制

摄食不同饵料对翘嘴鳜脑组织、肝组织中基因表达的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
382-391
栏目:
畜牧兽医·水产养殖
出版日期:
2026-02-28

文章信息/Info

Title:
Effects of different feeding regimens on gene expression in brain and liver tissues of mandarin fish (Siniperca chuatisi)
作者:
周扬达1; 2; 郑建波2; 李飞2; 刘士力2; 蒋文枰2; 迟美丽2; 程顺2; 张海琪1; 2
(1.浙江海洋大学, 浙江舟山316022;2.浙江省淡水水产研究所,浙江湖州313001)
Author(s):
ZHOU Yangda1; 2; ZHENG Jianbo2; LI Fei2; LIU Shili2; JIANG Wenping2; CHI Meili2; CHENG Shun2; ZHANG Haiqi1; 2
(1.Zhejiang Ocean University, Zhoushan 316022, China;2.Zhejiang Institute of Freshwater Fisheries, Huzhou 313001, China)
关键词:
翘嘴鳜; 不同摄食饵料; 转录组测序; 差异表达基因
Keywords:
Siniperca chuatisi; different feeding regimens; transcriptome sequencing; differentially expressed genes
分类号:
S917
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.017
文献标志码:
A
摘要:
为研究肉食性翘嘴鳜适应配合饲料及食性转换的分子调控机制,本研究采用转录组测序方法,对摄食活饵的对照组(LFSC)和摄食配合饲料的试验组(CFSC)翘嘴鳜的脑组织、肝组织的基因表达模式进行深入分析。结果表明,共得到有效数据约92.00 Gb,其中质量数大于20的碱基所占百分比(Q20)、质量数大于30的碱基所占百分比(Q30)分别在98.00%、96.00%以上,G+C含量区间为44.94%～50.19%。比较转录组分析结果显示,基于活饵组与饲料组的比较数据,从翘嘴鳜脑组织中共获得1 405个差异表达基因(Differentially expressed genes, DEG),其中486个DEG上调表达,919个DEG下调表达；基于活饵组与饲料组的比较数据,从翘嘴鳜肝组织中获得2 850个DEG,其中1 298个DEG上调表达,1 552个DEG下调表达。对翘嘴鳜脑组织、肝组织中的差异表达基因进行京都基因与基因组百科全书（KEGG）功能富集分析发现,翘嘴鳜肝组织中的DEG主要富集于类固醇生物合成、脂肪酸降解、丙酮酸代谢及抗原加工与呈递等信号通路,脑组织中的DEG主要富集于细胞因子-细胞因子受体互作等信号通路。进一步通过功能注释筛选到昼夜节律通路相关基因clockb、per1b、RORA,学习信号通路相关基因creb1b,视网膜光敏感性通路相关基因rdh8a、rgra,食欲调控通路相关基因tac1、eif4eb,代谢通路相关基因pck1等。筛选到的基因可作为参与决定翘嘴鳜独特食物偏好的候选基因。本研究结果可为下一步揭示翘嘴鳜适应人工配合饲料及摄食偏好的转变提供理论支撑,有助于培育易驯食人工配合饲料翘嘴鳜新品系。
Abstract:
To investigate the molecular mechanisms underlying the adaptation to formulated feed and dietary transition in carnivorous mandarin fish (Siniperca chuatsi), we performed deep transcriptome sequencing of brain and liver tissues from the live-food control group (LFSC) and the formulated-feed treatment group (CFSC). A total of approximately 92.00 Gb of clean data were obtained, with Q20≥98.00% and Q30≥96.00%, and the G+C content ranged from 44.94% to 50.19%. Comparative transcriptomic analysis identified 1 405 differentially expressed genes (DEGs) in the brain (LFSC vs. CFSC), with 486 up-regulated and 919 down-regulated. Similarly, 2 850 DEGs were identified in the liver, including 1 298 up-regulated and 1 552 down-regulated. Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that liver DEGs were significantly enriched in steroid biosynthesis, fatty-acid degradation, pyruvate metabolism and antigen processing and presentation, whereas brain DEGs were mainly enriched in the cytokine-cytokine receptor interaction pathway. Further functional annotation highlighted DEGs associated with circadian rhythm (clockb, per1b, RORA), learning/memory (creb1b), retinal phototransduction (rdh8a, rgra), appetite regulation (tac1, eif4eb) and metabolism (pck1). These genes represent candidate regulators of the species-specific food preference in mandarin fish. The findings provide a theoretical framework for elucidating the molecular basis of dietary adaptation and preference shift in mandarin fish, and will facilitate the breeding of novel strains that readily accept formulated feed.

参考文献/References:

[1]DING W D, ZHANG X H, ZHAO X M, et al. A chromosome-level genome assembly of the mandarin fish (Siniperca chuatsi)[J]. Frontiers in Genetics,2021,12:671650.
[2]CAI-WU Y Q D Z. On the classification and distribution of the sinipercinae fishes (Family Serranidae)[J]. Zoological Research, 1988, 9(2):113-125.
[3]HE S, LI L, LUY L Y, et al. Mandarin fish (Sinipercidae) genomes provide insights into innate predatory feeding[J]. Communications Biology,2020,3(1):361.
[4]班赛男,朱传忠,杨新冬,等. 摄食不同饵料对翘嘴鳜生长、体成分和消化酶活性的影响[J]. 淡水渔业,2020,50(1):93-100.
[5]LI Y, LI J Z, LU J T, et al. Effects of live and artificial feeds on the growth,digestion,immunity and intestinal microflora of mandarin fish hybrid (Siniperca chuatsi♀ × Siniperca scherzeri♂)[J]. Aquaculture Research,2017,48(8):4479-4485.
[6]HE J G, WANG S P, ZENG K, et al. Systemic disease caused by an iridovirus-like agent in cultured mandarinfish,Siniperca chuatsi (Basilewsky),in China[J]. Journal of Fish Diseases,2000,23(3):219-222.
[7]TAO J J, GUI J F, ZHANG Q Y. Isolation and characterization of a rhabdovirus from co-infection of two viruses in mandarin fish[J]. Aquaculture,2007,262(1):1-9.
[8]陈晓,孙成飞,董浚键,等. 摄食配合饲料翘嘴鳜快长组与慢长组转录组特征比较分析[J]. 基因组学与应用生物学,2023,42(5):501-517.
[9]吴遵霖,李蓓,李桂云,等. 鳜鱼配合饲料驯饲与养殖研究[J]. 淡水渔业,1996,26(1):16-19.
[10]LIANG X F, OKU H, OGATA H Y, et al. Weaning Chinese perch Siniperca chuatsi (Basilewsky) onto artificial diets based upon its specific sensory modality in feeding[J]. Aquaculture Research,2001,32:76-82.
[11]LIANG X F, LIN X T, LI S Q, et al. Impact of environmental and innate factors on the food habit of Chinese perch Siniperca chuatsi (Basilewsky) (Percichthyidae)[J]. Aquaculture Research,2008,39(2):150-157.
[12]PENG J, DOU Y Q, LIANG H, et al. Social learning of acquiring novel feeding habit in mandarin fish (Siniperca chuatsi)[J]. International Journal of Molecular Sciences,2019,20(18):4399.
[13]HE S, LIANG X F, SUN J, et al. Insights into food preference in hybrid F1 of Siniperca chuatsi (♀) × Siniperca scherzeri (♂) mandarin fish through transcriptome analysis[J]. BMC Genomics,2013,14:601.
[14]刘鼎瑞,欧阳号锋,黄景军,等. 基于转录组测序解析翘嘴鳜适应人工饲料的分子机制[J]. 海南热带海洋学院学报, 2022, 29(5): 1-11.
[15]CHEN Y X, CHEN Y S, SHI C M, et al. SOAPnuke:a MapReduce acceleration-supported software for integrated quality control and preprocessing of high-throughput sequencing data[J]. GigaScience,2018,7(1):1-6.
[16]KIM D, LANGMEAD B, SALZBERG S L. HISAT:a fast spliced aligner with low memory requirements[J]. Nature Methods,2015,12(4):357-360.
[17]LI B, DEWEY C N. RSEM:accurate transcript quantification from RNA-Seq data with or without a reference genome[J]. BMC Bioinformatics,2011,12(1):323.
[18]LOVE M I, HUBER W, ANDERS S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2[J]. Genome Biology,2014,15(12):550.
[19]潘教文,李臻,王庆国,等. NaCl处理谷子萌发期种子的转录组学分析[J]. 中国农业科学,2019,52(22):3964-3976.
[20]LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-△△Ct method[J]. Methods,2001,25(4):402-408.
[21]蒋阳阳,汪翔,吴明林,等. 鳜鱼配合饲料养殖经济效益分析及发展建议[J]. 安徽农业科学,2023,51(22):72-75.
[22]ORT G, LOVEJOY N R, SHI Q. Special issue on fish transcriptomics[J]. Genomics,2019,111(3):213-214.
[23]GUAN W Z, QIU G F, LIU F. Transcriptome analysis of the growth performance of hybrid mandarin fish after food conversion[J]. PLoS One,2020,15(10):e0240308.
[24]LI L, LIANG X F, HE S, et al. Transcriptome analysis of grass carp (Ctenopharyngodon idella) fed with animal and plant diets[J]. Gene,2015,574(2):371-379.
[25]ZHAO L L, LUO J, LIU Q, et al. Different diets can affect the digestion and immunity of common carp (Cyprinus carpio) according to enzyme activity assay and transcriptome sequencing[J]. Aquaculture,2020,523:735176.
[26]张煜,何中央,黄家庆,等. 配合饲料和活饵饲喂对翘嘴鳜肌肉代谢基因表达的影响研究[J]. 浙江农业科学,2023,64(6):1380-1384.
[27]ZHENG N, SCHULMAN B A, SONG L Z, et al. Structure of the Cul1-Rbx1-Skp1-F boxSkp2 SCF ubiquitin ligase complex[J]. Nature,2002,416(6882):703-709.
[28]AKASHI M, TSUCHIYA Y, YOSHINO T, et al. Control of intracellular dynamics of mammalian period proteins by casein kinase I ε (CKIε) and CKIδ in cultured cells[J]. Molecular and Cellular Biology,2002,22(6):1693-1703.
[29]ZHENG J B, YAO Y F, RUI Q L, et al. Effect of different feeding regimens on physiological indicators,intestinal transcriptome,and bacterial flora of mandarin fish (Siniperca chuatsi)[J]. Comparative Biochemistry and Physiology Part D:Genomics and Proteomics,2024,52:101301.
[30]LIANG X F, KIU J K, HUANG B Y. The role of sense organs in the feeding behaviour of Chinese perch[J]. Journal of Fish Biology,1998,52(5):1058-1067.
[31]ANDREWS Z B, LIU Z W, WALLLINGFORD N, et al. UCP2 mediates ghrelin’s action on NPY/AgRP neurons by lowering free radicals[J]. Nature,2008,454(7206):846-851.
[32]TAO Y X. The melanocortin-4 receptor:physiology,pharmacology,and pathophysiology[J]. Endocrine Reviews,2010,31(4):506-543.

备注/Memo

备注/Memo:
收稿日期：2025-03-03基金项目：重点研发计划项目“农业双强”专项(2024ZD2046)作者简介：周扬达(1999-),男,浙江建德人,硕士研究生,主要从事动物遗传育种及繁殖研究。(E-mail)344060212@qq.com通讯作者：张海琪,(E-mail)zmk407@126.com
更新日期/Last Update: 2026-03-16