[1]杜澳博,胡浩,王静,等.江苏省和山东省部分地区奶牛粪便中大肠埃希菌耐药性及分子流行特征[J].江苏农业学报,2026,42(02):357-365.[doi:doi:10.3969/j.issn.1000-4440.2026.02.014]
　DU Aobo,HU Hao,WANG Jing,et al.Drug resistance and molecular epidemiological characteristics of Escherichia coli in dairy cow feces in Jiangsu and Shandong provinces[J].,2026,42(02):357-365.[doi:doi:10.3969/j.issn.1000-4440.2026.02.014]
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江苏省和山东省部分地区奶牛粪便中大肠埃希菌耐药性及分子流行特征()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
357-365
栏目:
畜牧兽医·水产养殖
出版日期:
2026-02-28

文章信息/Info

Title:
Drug resistance and molecular epidemiological characteristics of Escherichia coli in dairy cow feces in Jiangsu and Shandong provinces
作者:
杜澳博1; 2; 胡浩2; 王静2; 杨祥牟2; 张莉莉2; 杨章平1; 李明勋1; 王冉2
(1.扬州大学动物科学与技术学院,江苏扬州225009；2.江苏省农业科学院农产品质量安全与营养研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室,江苏南京210014)
Author(s):
DU Aobo1; 2; HU Hao2; WANG Jing2; YANG Xiangmu2; ZHANG Lili2; YANG Zhangping1; LI Mingxun1; WANG Ran2
(1.College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety—State Key Laboraton Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China)
关键词:
大肠埃希菌; 全基因组测序; 耐药性分析; 毒力基因
Keywords:
Escherichia coli; whole genome sequencing; antimicrobial resistance analysis; virulence genes
分类号:
R446.5
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.014
文献标志码:
A
摘要:
为明确江苏省和山东省规模化养殖场奶牛粪便中大肠埃希菌的耐药情况与分子流行病学特征,本研究从江苏省徐州市、淮安市、宿迁市、盐城市和山东省日照市5市规模化养殖场采集腹泻奶牛粪便样品156份,使用伊红美蓝琼脂培养基分离大肠埃希菌,利用微量肉汤稀释法测定分离株对常见抗菌药物的耐药性,基于全基因组测序结果分析分离菌株耐药基因和毒力基因的携带情况及其分子分型和主要流行特征。结果表明,从156份奶牛粪便样品中共分离获得46株大肠埃希菌,分离率为29.49%,分离株对庆大霉素、氨苄西林、四环素和磺胺甲口恶唑的耐药率较高,分别为65.22%、63.04%、63.04%和60.81%；58.70%的分离株对3种及以上药物产生耐药性。分离株中共检测出44种耐药基因和57种毒力基因,耐药基因主要包括blaEC、blaCTX-M-55、blaNDM-5、blaNDM-6、aph(3’)-Ia、tet(A)、sul2、qnrS1和mph(A),检出率分别为100.00%、47.83%、6.52%、2.17%、30.43%、34.78%、30.43%、26.09%、32.61%,毒力基因主要包括espX1、fedC、sslE、iss、eae、astA、stxA1a、stxB1a、stxB2c,检出率分别为84.78%、80.43%、50.00%、34.78%、8.70%、4.34%、2.17%、2.17%、2.17%。从46株分离株中共预测到30种血清型和28种多位点序列分型(MLST),其中O101为主要流行血清型,占比21.74%；ST10为优势MLST,占比17.39%。分离株可分为4个进化分支,不同地区分离株之间遗传进化关系较远。本研究结果为江苏省和山东省地区奶牛大肠埃希菌引发的疾病的精准防治提供了参考依据。
Abstract:
To clarify the drug resistance and molecular epidemiological characteristics of Escherichia coli in dairy manure from large-scale farms in Jiangsu province and Shandong province, 156 fecal samples were collected from large-scale farms in Xuzhou, Huai’an, Suqian, Yancheng in Jiangsu province and Rizhao in Shandong province. Escherichia coli was isolated by Eosin Methylene Blue agar medium, and the drug resistance of the isolates to common antibiotics was determined by micro-broth dilution method. Based on the results of whole genome sequencing, the carrying status of drug resistance genes and virulence genes, molecular typing and main epidemic characteristics of the isolates were analyzed. The results showed that 46 strains of Escherichia coli were isolated from 156 cow feces samples, and the isolation rate was 29.49%. The resistance rates of the isolates to gentamicin, ampicillin, tetracycline and sulfamethoxazole were 65.22%, 63.04%, 63.04% and 60.81%, respectively. Additionally, 58.70% of the isolates demonstrated resistance to three or more drugs. A total of 44 drug resistance genes and 57 virulence genes were detected in the isolates. The drug resistance genes mainly included blaEC, blaCTX-M-55, blaNDM-5, blaNDM-6, aph(3’)-Ia, tet(A), sul2, qnrS1 and mph(A), and the detection rates were 100.00%, 47.83%, 6.52%, 2.17%, 30.43%, 34.78%, 30.43%, 26.09% and 32.61%, respectively. Virulence genes mainly included espX1, fedC, sslE, iss, eae, astA, stxA1a, stxB1a and stxB2c, with detection rates of 84.78%, 80.43%, 50.00%, 34.78%, 8.70%, 4.34%, 2.17%, 2.17% and 2.17%, respectively. A total of 30 serotypes and 28 multilocus sequence typing (MLST) types were predicted from 46 isolates. Among these, O101 was identified as the predominant serotype, accounting for 21.74%, and ST10 was the dominant MLST type, accounting for 17.39%. The isolates were classified into four phylogenetic clusters, with relatively distant genetic relationships observed among strains from different regions. The results of this study provide a reference for the precise prevention and control of diseases caused by Escherichia coli in dairy cows in Jiangsu and Shandong provinces.

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备注/Memo

备注/Memo:
收稿日期：2025-05-09基金项目：国家重点研发计划项目(2021YFE0101800、2022YFF1001200)作者简介：杜澳博(1999-),男,河南漯河人,硕士研究生,主要从事噬菌体研究。(E-mail)duaobo1101@163.com通讯作者：王冉,(E-mail)ranwang@jaas.ac.cn；李明勋,(E-mail)limingxun@live.com
更新日期/Last Update: 2026-03-16