[1]刘萍萍,孙阎,刘丹丹,等.亚麻E2F/DP转录因子基因家族鉴定及表达分析[J].江苏农业学报,2026,42(02):250-263.[doi:doi:10.3969/j.issn.1000-4440.2026.02.004]
　LIU Pingping,SUN Yan,LIU Dandan,et al.Identification and expression analysis of the E2F/DP transcription factor gene family in flax[J].,2026,42(02):250-263.[doi:doi:10.3969/j.issn.1000-4440.2026.02.004]
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亚麻E2F/DP转录因子基因家族鉴定及表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年02期
页码:
250-263
栏目:
遗传育种·生理生化
出版日期:
2026-02-28

文章信息/Info

Title:
Identification and expression analysis of the E2F/DP transcription factor gene family in flax
作者:
刘萍萍1; 2; 孙阎2; 刘丹丹1; 唐立郦1; 杨洌1; 程莉莉1; 康庆华1; 宋喜霞1; 姜忠娟2; 刘烨3
(1.黑龙江省农业科学院经济作物研究所,黑龙江哈尔滨150086；2.黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150081；3.东北林业大学生命科学学院,黑龙江哈尔滨150040；4.黑龙江省农业科学院草业研究所,黑龙江哈尔滨150086)
Author(s):
LIU Pingping1; 2; SUN Yan2; LIU Dandan1; TANG Lili1; YANG Lie1; CHENG Lili1; KANG Qinghua1; SONG Xixia1; JIANG Zhongjuan2; LIU Ye3
(1.Institute of Economic Crops, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China;2.College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150081, China;3.College of Life Sciences, Northeast Forestry University, Harbin 150040, China;4.Institute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China)
关键词:
亚麻; E2F/DP家族基因; 转录因子
Keywords:
flax; E2F/DP family genes; transcription factors
分类号:
S563.2
DOI:
doi:10.3969/j.issn.1000-4440.2026.02.004
文献标志码:
A
摘要:
为明确亚麻E2F/DP家族基因的生物信息学特征及其在作物生长发育过程中的影响机制,本研究通过生物信息学方法,分析亚麻E2F/DP家族基因结构、顺式作用元件、染色体定位、物种内和物种间共线性特征,及其编码蛋白质的理化性质和互作网络,并利用转录组测序(RNA-seq)技术分析不同激素处理下亚麻E2F/DP基因的表达模式。结果表明,亚麻基因组中共鉴定到14个E2F/DP家族基因,其中有7个属于E2F亚家族,3个属于DP亚家族,4个属于DEL亚家族；同一亚家族的E2F/DP基因结构及其编码蛋白质的保守基序分布、保守结构域高度相似。物种内共发现16对共线性基因,且存在共线性的两个基因位于不同的染色体上。亚麻E2F/DP家族基因启动子序列中含有丰富的光响应元件、激素响应元件、胁迫响应元件、发育过程特定元件等。3个DP亚家族蛋白质(Lus10014423、Lus10022620和Lus10023926)为关键枢纽蛋白。不同激素处理后,亚麻茎部中段、茎部下段与叶片中14个E2F/DP家族基因均呈现差异性表达,基于实时荧光定量PCR(qRT-PCR)得到的Lus10022620和Lus10039455基因相对表达量与转录组测序结果在变化趋势上基本一致。本研究结果为进一步开展激素对亚麻E2F/DP转录因子的调控机制研究提供了基础。
Abstract:
To clarify the bioinformatics characteristics of flax E2F/DP family genes and their mechanisms in crop growth and development, this study analyzed the gene structure, cis-acting elements, chromosomal localization and intra-species and inter-species collinearity of flax E2F/DP family, as well as the physicochemical properties and interaction networks of the encoded proteins by bioinformatics methods, and analyzed the expression pattern of flax E2F/DP genes under different hormone treatments by transcriptome sequencing (RNA-seq) technology. The results showed that a total of 14 E2F/DP family genes were identified in the flax genome, of which seven belonged to the E2F subfamily, three belonged to the DP subfamily, and four belonged to the DEL subfamily. Within the same subfamily, the E2F/DP gene structures, as well as the distribution of conserved motifs and conserved domains of the encoded proteins were highly similar. A total of 16 pairs of collinearity genes were found in the species, and two genes with collinearity were located on different chromosomes. The promoter sequences of flax E2F/DP family genes contained abundant light response elements, hormone response elements, stress response elements, development process specific elements and so on. Three DP subfamily proteins (Lus10014423, Lus10022620 and Lus10023926) were key hub proteins. After different hormone treatments, 14 E2F/DP genes in the middle and lower segments of flax stem and leaf tissues showed differential expression. The relative expression levels of Lus10022620 and Lus10039455 genes based on real-time fluorescence quantitative PCR (qRT-PCR) were basically consistent with the trend of transcriptome sequencing results. The results of this study provide a basis for further study on the regulation mechanism of hormones on flax E2F/DP transcription factors.

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备注/Memo

备注/Memo:
收稿日期:2025-08-27基金项目:黑龙江省农业科技创新跨越工程项目(CX23GG04)；国家麻类产业技术体系资助项目(CARS-16-S03)作者简介:刘萍萍(2000-),女,黑龙江哈尔滨人,硕士研究生,主要从事亚麻分子遗传育种研究。(E-mail)2310590367@qq.com通讯作者:袁红梅,(E-mail)yuanhm@haas.cn
更新日期/Last Update: 2026-03-16