[1]聂春雨,王日欣,凌磊,等.紫花苜蓿对盐碱地土壤理化性质和生物多样性的影响[J].江苏农业学报,2026,42(01):68-78.[doi:doi:10.3969/j.issn.1000-4440.2026.01.008]
　NIE Chunyu,WANG Rixin,LING Lei,et al.The impact of Medicago sativa L. on the physicochemical properties and biodiversity of saline-alkali soil[J].,2026,42(01):68-78.[doi:doi:10.3969/j.issn.1000-4440.2026.01.008]
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紫花苜蓿对盐碱地土壤理化性质和生物多样性的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年01期
页码:
68-78
栏目:
耕作栽培·资源环境
出版日期:
2026-01-31

文章信息/Info

Title:
The impact of Medicago sativa L. on the physicochemical properties and biodiversity of saline-alkali soil
作者:
聂春雨1; 2; 王日欣1; 凌磊1; 2; 殷亚杰1; 2; 陈乃钰1; 2; 任国领1; 2
(1.大庆师范学院生物工程学院,黑龙江大庆163712；2.黑龙江省油田应用化学与技术重点实验室,黑龙江大庆163712)
Author(s):
NIE Chunyu1; 2; WANG Rixin1; LING Lei1; 2; YIN Yajie1; 2; CHEN Naiyu1; 2; REN Guoling1; 2
(1.School of Biological Engineering, Daqing Normal University, Daqing 163712, China；2.Heilongjiang Provincial Key Laboratory of Oilfield Applied Chemistry and Technology, Daqing 163712, China)
关键词:
紫花苜蓿; 盐碱地; 土壤微生物; 土壤动物; 相关性分析
Keywords:
Medicago sativa L.; saline-alkali soil; soil microorganisms; soil animals; correlation analysis
分类号:
S156.4
DOI:
doi:10.3969/j.issn.1000-4440.2026.01.008
文献标志码:
A
摘要:
本研究利用16S rRNA高通量测序技术和土壤动物干漏斗分离法,系统分析了紫花苜蓿种植对盐碱地土壤环境、动物群落、微生物群落的影响。结果表明,与未种植紫花苜蓿盐碱地相比,种植紫花苜蓿盐碱地土壤脱氢酶(DHA)、蔗糖酶(SC)、脲酶(UE)、过氧化氢酶(CAT)、多酚氧化酶(PPO)、转化酶(AI)活性以及土壤全氮含量、速效氮含量、速效磷含量、速效钾含量均显著提高(P＜0.05),土壤pH值显著降低(P＜0.05)。这种土壤环境的变化直接驱动了微生物群落的重构。具体表现为,与未种植紫花苜蓿盐碱地相比,种植紫花苜蓿盐碱地土壤中黄色土源菌属(Flavisolibacter)、溶杆菌属(Lysobacter)、类固醇杆菌属(Steroidobacter)、Blastocatellaceae、RB41细菌相对丰度以及弯孢属(Curvularia)、黄杆菌属(Laetisaria)、Phaeomycocentrospora、Naganishia、Knufia真菌相对丰度显著上升(P＜0.05),Nitriliruptoraceae、Afifella、Tepidamorphus、Balneolaceae、硝化杆菌属(Nitrosococcus)细菌相对丰度以及Sodiomyces、黄囊伞菌属(Deconica)、假丝酵母菌属(Candida)、Robillarda、粪壳菌属(Sordaria)真菌相对丰度显著下降(P＜0.05)。土壤微生物群落与土壤动物群落呈现显著相关性。盲蛛目(Opiliones)、石蛃目(Microcoryphia)动物相对丰度分别与Nitriliruptoraceae、Afifella、Tepidamorphus、拜耶林克氏菌属(Balneolaceae)、硝化杆菌属(Nitrosococcus)细菌相对丰度呈显著负相关(P＜0.05),与类固醇杆菌属(Steroidobacter)细菌相对丰度呈显著正相关(P＜0.05)。盲蛛目(Opiliones)、石蛃目(Microcoryphia)动物相对丰度分别与粪壳菌属(Sodiomyces)、黄囊伞菌属(Deconic)、假丝酵母菌属(Candida)、Robillarda真菌相对丰度呈显著负相关(P＜0.05)。表明土壤动物群落参与了土壤微生物群落的调控,土壤动物与微生物的互作进一步影响环境因子,这种环境因子-微生物-动物的三元互作网络共同构成了紫花苜蓿改良盐碱地的生态基础。本研究结果为盐碱地生物改良提供了理论依据。
Abstract:
In this study, 16S rRNA high-throughput sequencing technology and the dry funnel separation method for soil animals were used to systematically analyze the effects of alfalfa (Medicago sativa L.) cultivation on the soil environment, animal communities, and microbial communities in saline-alkali soils. The results showed that compared with saline-alkali soils without alfalfa cultivation, the activities of soil dehydrogenase (DHA), sucrase (SC), urease (UE), catalase (CAT), polyphenol oxidase (PPO), and invertase (AI), as well as the contents of soil total nitrogen, available nitrogen, available phosphorus, and available potassium in saline-alkali soils with alfalfa cultivation were significantly increased (P＜0.05), while the soil pH value was significantly decreased (P＜0.05). These changes in the soil environment directly drove the reconstruction of microbial communities. Specifically, compared with saline-alkali soils without alfalfa cultivation, the relative abundances of bacteria such as Flavisolibacter, Lysobacter, Steroidobacter, Blastocatellaceae, and RB41, as well as fungi such as Curvularia, Laetisaria, Phaeomycocentrospora, Naganishia, and Knufia in saline-alkali soils with alfalfa cultivation were significantly increased (P＜0.05). In contrast, the relative abundances of bacteria such as Nitriliruptoraceae, Afifella, Tepidamorphus, Balneolaceae, and Nitrosococcus, as well as fungi such as Sodiomyces, Deconica, Candida, Robillarda, and Sordaria were significantly decreased (P＜0.05). There was a significant correlation between the soil microbial communities and the soil animal communities. The relative abundances of animals belonging to Opiliones and Microcoryphia were significantly negatively correlated with the relative abundances of bacteria such as Nitriliruptoraceae, Afifella, Tepidamorphus, Balneolaceae, and Nitrosococcus (P＜0.05), and significantly positively correlated with the relative abundance of Steroidobacter (P＜0.05). Additionally, the relative abundances of Opiliones and Microcoryphia animals were significantly negatively correlated with the relative abundances of fungi such as Sodiomyces, Deconica, Candida, and Robillarda (P＜0.05). These results indicate that the soil animal communities are involved in the regulation of the soil microbial communities, and the interaction between soil animals and microorganisms further affects environmental factors. This ternary interaction network of environmental factors-microorganisms-animals collectively constitutes the ecological basis for the improvement of saline-alkali soils by alfalfa. This study provides a theoretical basis for the biological improvement of saline-alkali soils.

参考文献/References:

[1]高攀攀. 改良剂施用对盐渍土壤碳氮转化的影响研究[D]. 泰安:山东农业大学,2023.
[2]朱烨. 丛生生物改良滨海盐碱地的效应及机制研究[D]. 南京:南京农业大学,2021.
[3]王世平,陈月,潘大伟,等. 盐碱地治理研究综述:现状、问题与对策[J]. 化工矿物与加工,2023,52(11):59-68.
[4]王艺璇,王珂,曲鲁平,等. 中国松嫩平原盐碱土固碳潜力过程及机理研究[J]. 中国农业资源与区划,2024,45(1):129-138.
[5]扶海超,夏阳,杨丹丹,等. 不同生物有机肥对土壤理化性状及烟叶生长发育的影响[J]. 安徽农业科学,2015,43(30):86-89.
[6]刘庚炜,高雅琪,邵泽璇,等. 土壤盐渍化修复技术研究进展[J]. 黑龙江农业科学,2024(1):99-107.
[7]ZHU D, SUN L, MAO L N, et al.Combined effects of cropping alfalfa (Medicago sativa L.) on the soil pore structure,microbial communities and organic carbon fractions in saline soils[J]. Applied Soil Ecology,2025,208:105993.
[8]王晓春,杨炜迪,高婷. 盐碱地紫花苜蓿根际土壤细菌群落多样性分析[J]. 北方园艺,2023(23):75-82.
[9]王晓春,高婷. 盐碱地紫花苜蓿根际土壤真菌多样性分析[J]. 农业与技术,2023,43(24):9-12.
[10]王永珍,冯怡琳,赵文智,等. 绿洲农田玉米和牧草种植对地表节肢动物群落结构的影响[J]. 中国生态农业学报(中英文),2023,31(11):1721-1732.
[11]HALLETT P D, MARIN M, BENDING G D, et al. Building soil sustainability from root-soil interface traits[J]. Trends in Plant Science,2022,27(7):688-698.
[12]孔亚丽,秦华,朱春权,等. 土壤微生物影响土壤健康的作用机制研究进展[J]. 土壤学报,2024,61(2):331-347.
[13]张怡洋,刘阳,阮迪,等. 艾丁湖可培养好氧嗜盐细菌多样性及抗辐射特征初探[J]. 湖北农业科学,2024,63(6):44-52.
[14]PIROMYOU P, BURANABANYAT B, TANTASAWAT P, et al. Effect of plant growth promoting rhizobacteria (PGPR) inoculation on microbial community structure in rhizosphere of forage corn cultivated in Thailand[J]. European Journal of Soil Biology,2010,47(1):44-54.
[15]李维娜,胡庆玲,郝转,等. 陕西省黄河湿地春季不同土地利用方式下中小型土壤动物多样性研究[J]. 湖北农业科学,2020,59(13):47-50.
[16]邱柏淞,宋鑫,谭智诚,等. 蚯蚓活动下矿物对凋落物分解及腐殖化的影响[J]. 农业环境科学学报,2024,43(9):2050-2059.
[17]SHARMA M, KHURANA H, SINGH D N, et al. The genus Sphingopyxis:systematics,ecology,and bioremediation potential—a review[J]. Journal of Environmental Management,2020,280:111744.
[18]EDWARDS J, JOHNSON C, SANTOS-MEDELLIN C, et al. Structure,variation,and assembly of the root-associated microbiomes of rice[J]. Proceedings of the National Academy of Sciences of the United States of America,2015,112(8):911-920.
[19]高明,周保同,魏朝富,等. 不同耕作方式对稻田土壤动物、微生物及酶活性的影响研究[J]. 应用生态学报,2004(7):1177-1181.
[20]HARTA I, SIMON B, VINOGRADOV S, et al. Collembola communities and soil conditions in forest plantations established in an intensively managed agricultural area[J]. Journal of Forestry Research,2021,32 :1819-1832.
[21]关松荫. 土壤酶及其研究方法[M]. 北京:中国农业出版社,1986.
[22]尹文英. 中国土壤动物检索图鉴[M]. 北京:科学出版社,1998.
[23]魏炳传. 《昆虫分类学》(修订版)介绍[J]. 植物保护,2018,254(3):217-217.
[24]田平雅. 耐盐植物根际细菌多样性研究及促生菌筛选和复合菌群构建[D]. 银川:宁夏大学,2019.

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备注/Memo

备注/Memo:
收稿日期：2024-12-09基金项目：黑龙江省自然科学基金项目(LH2021C002)；大庆市指导科技计划项目(zd-2023-12)；黑龙江省省属高等学校基本科研业务费项目(2023-KYYWF-0026)作者简介：聂春雨(1978-),女,内蒙古乌兰察布人,硕士,副教授,主要从事土壤微生物研究。(E-mail)niechunyu2009@163.com。王日欣为共同第一作者。通讯作者：殷亚杰,(E-mail)yinyajie2009@163.com
更新日期/Last Update: 2026-02-09