[1]张萱,罗文秀,徐萍,等.CO2浓度升高对灯盏花幼苗光合特性、抗氧化系统及有效成分积累的影响[J].江苏农业学报,2026,42(01):32-40.[doi:doi:10.3969/j.issn.1000-4440.2026.01.004]
　ZHANG Xuan,LUO Wenxiu,XU Ping,et al.Effects of elevated CO2 concentration on photosynthetic characteristics, antioxidant system and active ingredient accumulation in Erigeron breviscapus (Vaniot) Hand.-Mazz seedlings[J].,2026,42(01):32-40.[doi:doi:10.3969/j.issn.1000-4440.2026.01.004]
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CO2浓度升高对灯盏花幼苗光合特性、抗氧化系统及有效成分积累的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
42
期数:
2026年01期
页码:
32-40
栏目:
遗传育种·生理生化
出版日期:
2026-01-31

文章信息/Info

Title:
Effects of elevated CO2 concentration on photosynthetic characteristics, antioxidant system and active ingredient accumulation in Erigeron breviscapus (Vaniot) Hand.-Mazz seedlings
作者:
张萱1; 罗文秀1; 徐萍1; 米琪1; 于梦雯1; 王丰1; 欧征刚2; 郑国伟1; 陈佳3
(1.云南中医药大学中药学院,云南昆明650500；2.普拉底乡人民政府农业综合服务中心,云南怒江673501；3.云南中医药大学民族医药学院,云南昆明650500)
Author(s):
ZHANG Xuan1; LUO Wenxiu1; XU Ping1; MI Qi1; YU Mengwen1; WANG Feng1; OU Zhenggang2; ZHENG Guowei1; CHEN Jia3
(1.School of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming 650500, China；2.Agricultural Comprehensive Service Center, People’s Government of Puladi Township, Nujiang 673501, China；3.College of Ethnomedicine,Yunnan University of Chinese Medicine, Kunming 650500, China)
关键词:
灯盏花; CO2浓度; 光合作用; 生理生化; 野黄芩苷
Keywords:
Erigeron breviscapus (Vaniot) Hand.-Mazz; CO2 concentration; photosynthesis; physiology and biochemistry; scutellarin
分类号:
S681.7
DOI:
doi:10.3969/j.issn.1000-4440.2026.01.004
文献标志码:
A
摘要:
为探究CO2浓度对灯盏花生长发育的影响,本研究以灯盏花为试验材料,采用人工气候室调控CO2浓度,设置400 μmol/mol(CK)和800 μmol/mol(T)两个处理,处理60 d后,系统测定幼苗气体交换参数、光合色素含量、渗透调节物质含量、有效成分含量、酶活性等指标。结果表明,与CK相比,T处理灯盏花气孔密度、气孔长度、蒸腾速率和气孔导度分别显著下降19.43%、13.51%、69.09%和70.41%(P<0.05),胞间CO2浓度与水分利用率分别显著升高85.60%和231.60%(P<0.05)。表明高浓度CO2条件下,灯盏花通过缩小或者关闭气孔以减少水分流失,提升水分利用效率。与CK相比,T处理丙二醛(MDA)含量和抗坏血酸过氧化物酶(APX)活性分别显著升高145.74%和121.68%(P<0.05),超氧化物歧化酶(SOD)和过氧化物酶(POD)活性分别显著升高40.66%、12.78%(P<0.05),谷胱甘肽还原酶(GR)与硝酸还原酶(NR)活性分别显著下降62.53%和36.69%(P<0.05)。表明CO2浓度升高通过降低光合色素含量、抑制氮同化关键酶活性对灯盏花造成氧化胁迫,植株通过增强抗氧化酶活性减轻膜脂过氧化损伤。与CK相比,T处理野黄芩苷、叶绿素a、类胡萝卜素含量分别显著下降25.26%、22.64%、27.87%(P<0.05)。表明高浓度CO2条件下,灯盏花有效成分含量显著降低。本研究结果为灯盏花适应性栽培提供了理论依据。
Abstract:
To investigate the effects of CO2 concentration on the growth and development of Erigeron breviscapus, this study used E. breviscapus as the experimental material and regulated CO2 concentration in an artificial climate chamber. Two treatments were set up, namely 400 μmol/mol (CK) and 800 μmol/mol (T). After 60 days of treatment, a systematic determination was conducted on various indices of the seedlings, including gas exchange parameters, photosynthetic pigment content, osmotic adjustment substance content, active ingredient content, and enzyme activity. The results showed that compared with CK, the stomatal density, stomatal length, transpiration rate and stomatal conduc-tance of E. breviscapus under T treatment decreased significantly by 19.43%, 13.51%, 69.09% and 70.41%, respectively (P<0.05), while the intercellular CO2 concentration and water use efficiency increased significantly by 85.60% and 231.60%, respectively (P<0.05). These findings indicated that under elevated CO2 conditions, E. breviscapus reduced water loss and improved water use efficiency by narrowing or closing stomata. Compared with CK, the malondialdehyde (MDA) content and ascorbate peroxidase (APX) activity under T treatment increased significantly by 145.74% and 121.68%, respectively (P<0.05), the activities of superoxide dismutase (SOD) and peroxidase (POD) increased significantly by 40.66% and 12.78%, respectively (P<0.05). Whereas the activities of glutathione reductase (GR) and nitrate reductase (NR) decreased significantly by 62.53% and 36.69%, respectively (P<0.05). This suggested that elevated CO2 concentration induces oxidative stress in E. breviscapus by reducing photosynthetic pigment content and inhibiting the activity of key enzymes involved in nitrogen assimilation, and the plants alleviate membrane lipid peroxidation damage by enhancing the activity of antioxidant enzymes. In addition, compared with CK, the contents of scutellarin, chlorophyll a and carotenoids under T treatment decreased significantly by 25.26%, 22.64% and 27.87%, respectively(P<0.05), indicating that the content of active ingredients in E. breviscapus decreased significantly under elevated CO2 conditions. Overall, the results of this study provide a theoretical basis for the adaptive cultivation of Erigeron breviscapus.

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备注/Memo

备注/Memo:
收稿日期：2025-02-21基金项目：国家自然科学基金项目(82360751)；云南中医药大学高层次人才计划项目(2019YZG07)；云南青年精英人才项目(YNWR-QNBJ-2020-289)作者简介：张萱(1995-),女,贵州剑河人,硕士研究生,研究方向为药用植物栽培生理学。(E-mail)2428892896@qq.com通讯作者：郑国伟,(E-mail)2633822198@qq.com；陈佳,(E-mail)chenjia@ynutcm.edu.cn
更新日期/Last Update: 2026-02-09