[1]沈玉平,袁志辉,刘弘承,等.球黑孢菌Gbh45菌株抗姜青枯菌活性物质分离鉴定及抗菌机制[J].江苏农业学报,2025,(11):2134-2145.[doi:doi:10.3969/j.issn.1000-4440.2025.11.007]
　SHEN Yuping,YUAN Zhihui,LIU Hongcheng,et al.Isolation, identification and antibacterial mechanism of active substances from Nigrospora sphaerica Gbh45 strain against Ralstonia solanacearum[J].,2025,(11):2134-2145.[doi:doi:10.3969/j.issn.1000-4440.2025.11.007]
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球黑孢菌Gbh45菌株抗姜青枯菌活性物质分离鉴定及抗菌机制()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2134-2145
栏目:
植物保护
出版日期:
2025-11-30

文章信息/Info

Title:
Isolation, identification and antibacterial mechanism of active substances from Nigrospora sphaerica Gbh45 strain against Ralstonia solanacearum
作者:
沈玉平1; 袁志辉1; 2; 刘弘承1; 邵金华1; 赵昌会1; 何福林1; 2; 张祖姣1
(1.湖南科技学院化学与生物工程学院,湖南永州425199；2.湖南省银杏工程技术研究中心,湖南永州425199)
Author(s):
SHEN Yuping1; YUAN Zhihui1; 2; LIU Hongcheng1; SHAO Jinhua1; ZHAO Changhui1; HE Fulin1; 2; ZHANG Zujiao1
(1.School of Chemistry and Bioengineering, Hunan University of Science and Engineering, Yongzhou 425199, China;2.Hunan Provincial Engineering Research Center for Ginkgo Biloba, Yongzhou 425199, China)
关键词:
球黑孢菌; 生姜; 青枯菌; 活性次生代谢物; 抗菌机制
Keywords:
Nigrospora sphaerica; ginger; Ralstonia solanacearum; active secondary metabolites; antibacterial mechanism
分类号:
S482+7
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.007
文献标志码:
A
摘要:
本研究旨在确定球黑孢菌(Nigrospora sphaerica)Gbh45菌株发酵液中抗姜青枯菌(Ralstonia solanacearum)的活性成分,并揭示其抗菌作用机制。为此,采用硅胶柱层析、半制备高效液相色谱法(HPLC)和核磁共振波谱法对球黑孢菌Gbh45菌株发酵液抗菌活性成分进行分离鉴定。通过测定姜青枯菌的生长情况、最小抑菌质量浓度(MIC)和半抑菌质量浓度(IC50)评价球黑孢菌Gbh45菌株活性次生代谢物的抗菌效果。进一步通过检测细胞膜完整性、细胞壁破损情况和胞内活性氧(ROS)水平探究其抗菌机制。结果显示,球黑孢菌Gbh45菌株的抗菌活性次生代谢物为蒽醌类化合物9α-hydroxydihydrodesoxybostrycin(9α-HDDB),对姜青枯菌的最小抗菌质量浓度仅为25000 μg/mL,半抑菌质量浓度为8974 μg/mL。9α-HDDB主要通过破坏细胞膜完整性,导致细胞壁破损,诱导细胞积累活性氧(ROS)的方式来抑制姜青枯菌生长。此外,9α-HDDB还对食源性病原菌大肠杆菌O157∶H7菌株和金黄色葡萄球菌展现出良好的抑制效果,显示其作为青枯菌生物农药和食品抗菌剂的巨大应用前景。
Abstract:
The aim of this study is to identify the active components in the fermentation broth of Nigrospora sphaerica Gbh45 strain that are effective against Ralstonia solanacearum, and to elucidate their antibacterial mechanism. Accordingly, we isolated and identified the antimicrobial active components in the fermentation broth of Nigrospora sphaerica Gbh45 strain using silica gel column chromatography, semi-preparative high-performance liquid chromatography (HPLC), and nuclear magnetic resonance (NMR) spectroscopy. We evaluated the antibacterial effects of the active secondary metabolites from Nigrospora sphaerica Gbh45 strain by measuring the growth curves of Ralstonia solanacearum, along with the minimum inhibitory concentration (MIC) and half-maximal inhibitory concentration (IC50). Moreover, we further investigated the antibacterial mechanism by assessing cell membrane integrity, cell wall damage, and intracellular reactive oxygen species (ROS) levels. The results indicated that the antimicrobial secondary metabolite from Nigrospora sphaerica Gbh45 was identified as an anthraquinone compound, 9α-hydroxydihydrodesoxybostrycin (9α-HDDB). It exhibited a minimum inhibitory concentration of 25000 μg/mL and a half-maximal inhibitory concentration of 8974 μg/mL against Ralstonia solanacearum. 9α-HDDB inhibits the growth of Ralstonia solanacearum primarily by disrupting cell membrane integrity, leading to cell wall damage and thereby inducing the accumulation of intracellular reactive oxygen species (ROS). The results showed that 9α-HDDB also exhibited strong inhibitory effects against the foodborne pathogens Escherichia coli O157∶H7 and Staphylococcus aureus, indicating its significant potential for development as both a biocontrol agent against Ralstonia solanacearum and a food antimicrobial agent.

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备注/Memo

备注/Memo:
收稿日期：2024-12-19基金项目：湖南省教育厅科学研究项目(22B0804)；湖南省自然科学基金项目(2024JJ7185、2024JJ7196)；湖南省普通高校教学改革研究项目(HNJG-20231105)作者简介：沈玉平(1981-),男,湖南永州人,博士,副教授,研究方向为农业工程。(E-mail)shenyup@mail3.sysu.edu.cn通讯作者：张祖姣,(E-mail)zhang_zujiao@126.com
更新日期/Last Update: 2025-12-18