[1]刘亚楠,史宝,杨柳,等.辣椒斑驳病毒江苏南京分离物的分子鉴定与系统进化分析[J].江苏农业学报,2025,(11):2127-2133.[doi:doi:10.3969/j.issn.1000-4440.2025.11.006]
　LIU Yanan,SHI Bao,YANG Liu,et al.Molecular identification and phylogenetic analysis of a Jiangsu Nanjing isolate of pepper mottle virus[J].,2025,(11):2127-2133.[doi:doi:10.3969/j.issn.1000-4440.2025.11.006]
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辣椒斑驳病毒江苏南京分离物的分子鉴定与系统进化分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年11期
页码:
2127-2133
栏目:
植物保护
出版日期:
2025-11-30

文章信息/Info

Title:
Molecular identification and phylogenetic analysis of a Jiangsu Nanjing isolate of pepper mottle virus
作者:
刘亚楠1; 史宝1; 2; 杨柳1; 任春梅1; 李硕1; 孙枫1; 季英华1; 2
(1.江苏省农业科学院植物保护研究所,江苏南京210014；2.青海大学农林科学院,青海西宁810016)
Author(s):
LIU Yanan1; SHI Bao1; 2; YANG Liu1; REN Chunmei1; LI Shuo1; SUN Feng1; JI Yinghua1; 2
(1.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China; 2.Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China)
关键词:
辣椒斑驳病毒; 病毒病; 基因组克隆; 进化分析
Keywords:
pepper mottle virus; viral disease; genomic cloning; evolutionary analysis
分类号:
S436.418.1
DOI:
doi:10.3969/j.issn.1000-4440.2025.11.006
文献标志码:
A
摘要:
本研究从江苏南京表现叶片卷曲矮缩症状的辣椒中鉴定出辣椒斑驳病毒（PepMoV）江苏分离物PepMoV-JS。PepMoV-JS侵染本氏烟后导致叶片卷曲、黄化及严重矮化。该病毒基因组全长9 461 bp,编码10个成熟蛋白。系统发育分析结果表明,辣椒斑驳病毒分为两大组群:辣椒斑驳病毒中国安徽、湖南、江苏分离物和韩国分离物聚为Ⅰ组群,辣椒斑驳病毒美国分离物、德国分离物和中国台湾分离物聚为Ⅱ组群。辣椒斑驳病毒中国分离物遗传相似度较低,群体多样性高。PepMoV-JS基因组中未检测到重组事件,表明其基因结构相对稳定。本研究结果为解析PepMoV在中国的分布、进化特征及制定有效防治策略提供理论依据。
Abstract:
In this study, a Jiangsu isolate of pepper mottle virus (PepMoV-JS) was identified from pepper plants showing leaf curling and stunting symptoms in Nanjing, Jiangsu province. Infection of Nicotiana benthamiana by PepMoV-JS resulted in leaf curling, yellowing, and severe stunting. The complete genome of PepMoV-JS is 9 461 bp in length, encoding 10 mature proteins. Phylogenetic analysis revealed that PepMoV isolates were clustered into two major groups: Group Ⅰ included isolates from Anhui (China), Hunan (China), Jiangsu (China) and the Republic of Korea, while Group Ⅱ comprised isolates from the United States, Germany, and Taiwan (China). Chinese PepMoV isolates exhibited low genetic similarity and high population diversity. No recombination events were detected in the genome of PepMoV-JS, indicating a relatively stable genetic structure. The findings of this study provide a theoretical basis for understanding the distribution, evolutionary characteristics of PepMoV in China, and formulating effective control strategies.

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备注/Memo

备注/Memo:
收稿日期：2025-04-01基金项目：江苏省自然科学基金项目(BK20231386)作者简介：刘亚楠(1997-),女，河南驻马店人,硕士研究生,主要从事作物病毒防控技术研究。(E-mail)2228239761@qq.com通讯作者：孙枫,(E-mail)sunfeng1201@126.com；季英华,(E-mail)jiyinghua@jaas.ac.cn
更新日期/Last Update: 2025-12-18