[1]金嘉凤,沈建兴,沈成,等.Bt Cry毒素广谱检测的胶体金侧流免疫层析试纸研究与设计[J].江苏农业学报,2025,(10):2057-2067.[doi:doi:10.3969/j.issn.1000-4440.2025.10.019]
　JIN Jiafeng,SHEN Jianxing,SHEN Cheng,et al.Research and design of colloidal gold lateral flow immunoassay strips for broad-spectrum detection of Bt Cry toxins[J].,2025,(10):2057-2067.[doi:doi:10.3969/j.issn.1000-4440.2025.10.019]
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Bt Cry毒素广谱检测的胶体金侧流免疫层析试纸研究与设计()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
2057-2067
栏目:
加工贮藏·质量安全
出版日期:
2025-10-31

文章信息/Info

Title:
Research and design of colloidal gold lateral flow immunoassay strips for broad-spectrum detection of Bt Cry toxins
作者:
金嘉凤1; 2; 3; 沈建兴1; 2; 沈成1; 3; 陈蔚1; 孙吉1; 4; 徐重新1; 2; 谢雅晶1; 张霄1; 朱庆1; 刘媛1; 2; 刘贤金1
(1.江苏省农业科学院农产品质量安全与营养研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室,江苏南京210014；2.江苏大学食品与生物工程学院,江苏镇江212013；3.南京农业大学植物保护学院,江苏南京211800；4.钟山职业技术学院健康管理与康复学院,江苏南京210049)
Author(s):
JIN Jiafeng1; 2; 3; SHEN Jianxing1; 2; SHEN Cheng1; 3; CHEN Wei1; SUN Ji1; 4; XU Chongxin1; 2; XIE Yajing1; ZHANG Xiao1; ZHU Qing1; LIU Yuan1; 2; LIU Xianjin
(1.Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety——State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China;2.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;3.College of Plant Protection, Nanjing Agricultural University, Nanjing 211800， China;4.School of Health Management and Rehabilitation, Zhongshan Vocational College, Nanjing 210049, China)
关键词:
Bt Cry毒素; 检测试纸; 侧流免疫层析; 抗体; 广谱检测
Keywords:
Bt Cry toxins; test strip; lateral flow immunoassay; antibody; broad-spectrum detection
分类号:
TS207.5
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.019
文献标志码:
A
摘要:
Bt Cry毒素是转基因农产品及其产地环境安全监管的重要对象。为实现转基因农产品的Bt Cry毒素快速检测,本研究基于抗体的胶体金侧流免疫层析法,以前期制备获得的具备Bt Cry毒素广谱识别能力的单克隆抗体为检测抗体,将其标记胶体金形成金标探针；同时分别以前期制备获得的具备Bt Cry毒素广谱识别能力的多克隆抗体为检测线（T线)上的捕获抗体、以商品化的羊抗鼠二抗为质控线（C线)上的质控抗体,进而依托硝化纤维膜等组件构建形成胶体金免疫层析检测试纸,并对其检测性能进行试验。结果表明,本研究构建设计的胶体金免疫层析检测试纸对Cry1Ab、Cry1Ac、Cry1Ah、Cry1B、Cry1C和Cry1F的最低肉眼检测线分别为0.5μg/mL、0.1μg/mL、0.5μg/mL、1.0μg/mL、0.5μg/mL和0.5 μg/mL,说明本研究研发的检测试纸可用于Bt Cry毒素的广谱快速检测,具有较高的应用价值。
Abstract:
Bt Cry toxins are important objects of environmental safety supervision of genetically modified agricultural products and their producing areas. In order to realize the rapid detection of Bt Cry toxins in genetically modified agricultural products, this study was conducted based on antibody-based colloidal gold lateral flow immunochromatography, using the monoclonal antibody with broad-spectrum recognition ability of Bt Cry toxins obtained in the previous preparation as the detection antibody, and label-ing it with colloidal gold to form a gold-labeled probe. At the same time, the polyclonal antibody with broad-spectrum recognition ability of Bt Cry toxins obtained in the previous preparation was used as the capture antibody on the test line (T line), and the commercial sheep anti-mouse monoclonal antibody was used as the quality control antibody on the quality control line (C line). Then, the colloidal gold immunochromatographic test strip was constructed based on nitrocellulose membrane and other components, and its detection performance was tested. The results showed that the minimum visual detection lines of the colloidal gold immunochromatographic test strips for Cry1Ab, Cry1Ac, Cry1Ah, Cry1B, Cry1C and Cry1F were 0.5 μg/mL, 0.1 μg/mL, 0.5 μg/mL, 1.0 μg/mL, 0.5 μg/mL and 0.5 μg/mL, respectively. These results indicate that the test strips developed in this study can be used for broad-spectrum rapid detection of Bt Cry toxins and have high application value.

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备注/Memo

备注/Memo:
收稿日期:2024-12-01基金项目:国家重点研发计划政府间国际合作重点专项(2023YFE0109400)；江苏省自然科学基金面上项目(BK20231384)；江苏省农业科技自主创新基金项目[CX(22)1009]作者简介:金嘉凤(1999-),女,江苏南通人,博士研究生,主要从事昆虫分子毒理学研究。(E-mail)jinjiafengjjf@163.com通讯作者:徐重新,(E-mail)hhxyxcx@163.com；刘媛,(E-mail) liuyuan@jaas.ac.cn
更新日期/Last Update: 2025-11-17