[1]刘东海,梁思威,张智,等.秸秆还田配施氮肥对冬油菜根际土壤微生物群落的影响[J].江苏农业学报,2025,(10):1962-1972.[doi:doi:10.3969/j.issn.1000-4440.2025.10.010]
　LIU Donghai,LIANG Siwei,ZHANG Zhi,et al.Effects of straw returning combined with nitrogen fertilizer application on the rhizosphere soil microbial community of winter rapeseed[J].,2025,(10):1962-1972.[doi:doi:10.3969/j.issn.1000-4440.2025.10.010]
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秸秆还田配施氮肥对冬油菜根际土壤微生物群落的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
1962-1972
栏目:
耕作栽培·资源环境
出版日期:
2025-10-31

文章信息/Info

Title:
Effects of straw returning combined with nitrogen fertilizer application on the rhizosphere soil microbial community of winter rapeseed
作者:
刘东海1; 梁思威2; 张智1; 肖卓熙1; 乔艳1; 任健1; 赵书军1; 胡诚1
(1.湖北省农业科学院植保土肥研究所,湖北武汉430064;2.酵母功能湖北省重点实验室,湖北宜昌430000)
Author(s):
LIU Donghai1; LIANG Siwei2; ZHANG Zhi1; XIAO Zhuoxi1; QIAO Yan1; REN Jian1; ZHAO Shujun1; HU Cheng1
(1.Institute of Plant Protection and Soil Fertilizer, Hubei Academy of Agricultural Sciences, Wuhan 430064, China；2.The Hubei Provincial Key Laboratory of Yeast Function, Yichang 430000, China)
关键词:
秸秆还田; 微生物群落结构; 冬油菜; 环境因子
Keywords:
straw return; microbial community structure; winter rapeseed; environmental factors
分类号:
S565.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.010
文献标志码:
A
摘要:
为探究施肥与秸秆还田对冬油菜根际土壤养分、产量及微生物群落特征的影响,本研究设置4个处理,分别为不施用秸秆和氮肥(CK)、施用秸秆处理(T1)、施用氮肥处理(T2)、同时施用秸秆和氮肥处理(T3)。利用Illumina高通量测序技术对细菌16S rRNA基因和真菌ITS区域进行测序,系统分析冬油菜根际土壤细菌与真菌群落的α多样性、群落结构及其与环境因子的关系,并同步测定土壤理化性质与冬油菜产量。研究结果表明,T3处理油菜产量和土壤真菌群落丰度显著高于CK、T1处理、T2处理(P＜0.05)。T3处理土壤富集了暗球腔菌属(Phaeosphaeria)和枝孢菌属(Cladosporium)真菌群落,这2个菌属的真菌大多为病原微生物,可能会造成油菜病害发生。环境因子与微生物群落的网络分析结果表明,土壤碱解氮含量、全氮含量、有机碳含量和有效磷含量这4个环境因子与细菌和真菌群落相对丰度具有较高的关联度。土壤碱解氮含量与节杆菌属(Arthrobacter)、黄杆菌属(Flavobacterium)细菌群落相对丰度呈显著正相关(P＜0.05)，这些菌属细菌可能参与了作物氮素转化过程。本研究结果为秸秆资源合理利用、改善土壤质量和提高作物产量提供了理论依据。
Abstract:
To investigate the effects of fertilization and straw returning on rhizosphere soil nutrients, yield, and microbial community characteristics of winter rapeseed, this study set up four treatments, namely: no straw application and no nitrogen fertilizer application (CK), straw application treatment (T1), nitrogen fertilizer application treatment (T2), and combined application of straw and nitrogen fertilizer treatment (T3). Using Illumina high-throughput sequencing technology, the bacterial 16S rRNA gene and fungal ITS region were sequenced. A systematic analysis was conducted on the α-diversity and community structure of bacterial and fungal communities in winter rapeseed rhizosphere soil, as well as their relationships with environmental factors. Meanwhile, soil physicochemical properties and winter rapeseed yield were determined synchronously. The results showed that the rapeseed yield and soil fungal community abundance in the T3 treatment were significantly higher than those in the CK, T1, and T2 treatments (P＜0.05). The T3 treatment led to the enrichment of fungal communities such as Phaeosphaeria and Cladosporium in the soil. Most fungi of these two genera are pathogenic microorganisms, which may cause rapeseed diseases. Network analysis of environmental factors and microbial communities revealed that four environmental factors (soil alkali-hydrolyzable nitrogen content, total nitrogen content, organic carbon content, and available phosphorus content) exhibited stronger associations with the relative abundance of both bacterial and fungal communities. The soil alkali-hydrolyzable nitrogen content was significantly positively correlated with the relative abundances of bacteria of the genera Arthrobacter and Flavobacterium (P＜0.05). Bacteria of these genera may be involved in the crop nitrogen transformation process. The findings of this study provide a theoretical basis for the rational utilization of straw resources, improvement of soil quality, and increase of crop yield.

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备注/Memo

备注/Memo:
收稿日期：2025-05-25基金项目：国家农业科技项目(20221805)；湖北省科技重大专项(2023BBA003)；国家重点研发计划项目(2024YFD1900100)作者简介：刘东海(1984-),男,河北邯郸人,硕士,副研究员,主要研究土壤肥料与高效施肥。(E-mail)396520042@qq.com通讯作者：胡诚,(E-mail)huchenghxz@163.com
更新日期/Last Update: 2025-11-17