[1]张玉洁,王荣华,王茂芊.氧化型谷胱甘肽引发对甜菜种子萌发及幼苗生理特性的影响[J].江苏农业学报,2025,(10):1899-1906.[doi:doi:10.3969/j.issn.1000-4440.2025.10.003]
　ZHANG Yujie,WANG Ronghua,WANG Maoqian.Effects of oxidized glutathione priming on seed germination and seedling physiological characteristics of sugar beet[J].,2025,(10):1899-1906.[doi:doi:10.3969/j.issn.1000-4440.2025.10.003]
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氧化型谷胱甘肽引发对甜菜种子萌发及幼苗生理特性的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年10期
页码:
1899-1906
栏目:
遗传育种·生理生化
出版日期:
2025-10-31

文章信息/Info

Title:
Effects of oxidized glutathione priming on seed germination and seedling physiological characteristics of sugar beet
作者:
张玉洁1; 王荣华2; 王茂芊1
(1.黑龙江大学现代农业与生态环境学院,黑龙江哈尔滨150080；2.石河子农业科学研究院,新疆石河子832099)
Author(s):
ZHANG Yujie1; WANG Ronghua2; WANG Maoqian1
(1.College of Advanced Agriculture and Ecological Environment, Heilongjiang University, Harbin 150080, China;2.Shihezi Academy of Agricultural Sciences, Shihezi 832099, China)
关键词:
甜菜; 氧化型谷胱甘肽; 种子引发; 萌发; 幼苗生理
Keywords:
sugar beet; oxidized glutathione; seed priming; germination; seedling physiology
分类号:
S566.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.10.003
文献标志码:
A
摘要:
为明确氧化型谷胱甘肽(GSSG)引发对甜菜种子萌发能力及幼苗生理特征的影响,本研究以甜菜品种801的种子为材料,设置6个不同质量浓度(10 mg/L、20 mg/L、50 mg/L、100 mg/L、200 mg/L、500 mg/L) GSSG溶液和2个浸种时间(16 h和24 h)的引发处理,分析不同质量浓度GSSG溶液及不同引发时间对甜菜种子萌发能力及幼苗生理指标的影响。结果表明,与未引发对照(CK)相比,不同质量浓度GSSG引发16 h和24 h处理总体上能显著提高甜菜种子发芽指数和活力指数,降低甜菜种子电导率,而对发芽率无显著影响。不同引发处理甜菜幼苗抗氧化酶活性、丙二醛(MDA)含量、H2O2含量存在较大变化。与CK相比,20mg/L、50mg/L、100mg/L GSSG引发24 h处理总体上能显著提升甜菜幼苗抗氧化酶活性、降低H2O2含量和MDA含量,而10 mg/L和20 mg/L GSSG引发16 h处理、200mg/L和500 mg/L GSSG引发24 h处理则总体上能降低抗氧化酶活性，增加H2O2含量和MDA含量。大部分引发处理甜菜幼苗的可溶性糖含量和可溶性蛋白含量呈增加趋势。总之,大部分GSSG引发处理能修复细胞膜损伤、调控氧化应激和促进渗透物质积累来提升种子活力,其中,20 mg/L GSSG引发24 h的处理效果较好。
Abstract:
To clarify the effects of oxidized glutathione (GSSG) priming on seed germination ability and seedling physiological characteristics of sugar beet, the seeds of sugar beet 801 were used as the experimental material in this study. Six different mass concentrations (10 mg/L, 20 mg/L, 50 mg/L, 100 mg/L, 200 mg/L, 500 mg/L ) of GSSG solution and two durations (16 h and 24 h) of priming treatment were set up to analyze their effects on seed germination ability and seedling physiological indexes of sugar beet. The results showed that compared with the non-soaked control (CK), the germination index and vigor index of sugar beet seeds were significantly increased, and the conductivity of sugar beet seeds was decreased by priming with different concentrations of GSSG solution for 16 h and 24 h, but there was no significant effect on germination rate. Under different priming treatments, the antioxidant enzyme activity, malondialdehyde (MDA) content and H2O2 content of sugar beet seedlings changed greatly. Compared with CK, treatments with 20 mg/L, 50 mg/L, 100 mg/L GSSG solution for priming 24 h could significantly improve the antioxidant enzyme activity of sugar beet seedlings, reduce H2O2 content and MDA content, while treatments with 10 mg/L and 20 mg/L GSSG solution for priming 16 h, 200 mg/L and 500 mg/L GSSG solution for priming 24 h could reduce the antioxidant enzyme activity, increase H2O2 content and MDA content. The soluble sugar content and soluble protein content of sugar beet seedlings under most priming treatments showed an increasing trend. In conclusion, most GSSG priming treatments can improve seed vigor by repairing membrane damage, regulating oxidative stress and promoting the accumulation of osmotic substances. Among them, the treatment with 20 mg/L GSSG solution for 24 h is identified as the most effective.

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备注/Memo

备注/Memo:
收稿日期：2025-04-24基金项目：师市计划项目(2024ZDNY11)；财政部和农业农村部国家现代农业产业技术体系(糖料)子项目(CARS-17011004、CARS-170113-1)；财政部和农业农村部国家现代农业产业技术体系(糖料)建设项目(CARS-170102)；国家自然科学基金面上项目(32272148)；黑龙江省博士后面上项目(LBH-Z22246)作者简介：张玉洁(2001-),女,山西晋城人,硕士研究生,研究方向为甜菜种子引发和遗传育种。(E-mail)1917699718@qq.com通讯作者：王茂芊,(E-mail)haixiang80@166.com
更新日期/Last Update: 2025-11-17