[1]张泽锦,梁颖,唐丽,等.外源赤霉素对三青莴笋拔节影响及转录组初步分析[J].江苏农业学报,2025,(09):1822-1832.[doi:doi:10.3969/j.issn.1000-4440.2025.09.017]
　ZHANG Zejin,LIANG Ying,TANG Li,et al.Preliminary analysis of the effects of exogenous gibberellin on stem elongation of 3-green asparagus lettuce and its transcriptome[J].,2025,(09):1822-1832.[doi:doi:10.3969/j.issn.1000-4440.2025.09.017]
点击复制

外源赤霉素对三青莴笋拔节影响及转录组初步分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1822-1832
栏目:
园艺
出版日期:
2025-09-30

文章信息/Info

Title:
Preliminary analysis of the effects of exogenous gibberellin on stem elongation of 3-green asparagus lettuce and its transcriptome
作者:
张泽锦1; 2; 梁颖1; 2; 唐丽1; 2; 李家慧3
(1.四川省农业科学院园艺研究所/果蔬园艺作物种质创新与利用四川省重点实验室, 四川 成都, 610066; 2. 四川省蔬菜工程技术研究中心, 四川 彭州, 611934; 3. 绵阳市农业科学研究院, 四川 绵阳, 621023)
Author(s):
ZHANG Zejin1; 2; LIANG Ying1; 2; TANG Li1; 2; LI Jiahui3
(1.Horticultural Crops Germplasm Innovation and Utilization Key Laboratory of Sichuan Province/Horticulture Research Institute, Sichuan Academy of Agricultural Sciences, Chengdu 610066, China;2.Sichuan Province Engineering Technology Research Center of Vegetables, Pengzhou 611934, China;3.Mianyang Academy of Agricultural Sciences, Mianyang 621023, China)
关键词:
三青莴笋; 拔节; 转录组; 差异表达基因
Keywords:
3-green asparagus lettuce; stem elongation; transcriptome; differentially expressed genes
分类号:
S636.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.017
文献标志码:
A
摘要:
为探究外源赤霉素对三青(青皮、青肉、青叶)莴笋拔节的影响机制,本研究以2种不同拔节能力的三青莴笋品种天香青和高山青为试验材料,以喷施清水为对照(CK),分别进行喷施赤霉素处理,分析赤霉素对莴笋生长的影响。结果表明,喷施赤霉素处理天香青和高山青的株高分别比CK增加61.74%和32.51%,茎叶鲜重比分别增加56.33%和40.35%,但对单株地上部分鲜重无显著影响。差异表达基因GO分析结果显示,2个莴笋品种喷施赤霉素处理分别与CK在生物学过程的差异主要在细胞形成过程,而2个品种在生物学过程的差异主要在细胞核和DNA等方面。与拔节有关的差异表达基因主要集中在二萜生物合成(Diterpenoid biosynthesis)途径。实时荧光PCR验证结果显示,喷施赤霉素处理天香青和高山青茎尖GA3ox基因相对表达量分别比CK降低55.4%和69.6%；拔节能力较好的高山青莴笋GA20ox和KAO基因相对表达量高于拔节能力弱的天香青莴笋。综上,外源赤霉素能影响莴笋内源赤霉素合成途径中关键基因的表达,三青莴笋拔节能力与内源赤霉素合成关键基因LsGA20ox1表达和赤霉素代谢有关的转录因子基因表达有关。
Abstract:
To explore the mechanism of exogenous gibberellin on stem elongation of 3-green (green peel, green flesh, green leaf) asparagus lettuce, two varieties of 3-green asparagus lettuce with different stem elongation ability, Tianxiangqing and Gaoshanqing, were used as experimental materials, and water was sprayed as control (CK). The effects of gibberellin on the growth of asparagus lettuce were analyzed. The results showed that compared with the CK， the plant height of Tianxiang-qing and Gaoshanqing increased by 61.74% and 32.51%, respectively, and the stem-to-leaf fresh weight ratio increased by 56.33% and 40.35% respectively, but there was no significant effect on the fresh weight of aboveground parts per plant. GO analysis of differentially expressed genes showed that the differences between gibberellin treatment and CK in the two asparagus lettuce varieties were mainly enriched in the biological process of cellular formation, while the differences between the two varieties were mainly associated with muclear components and DNA-related biological processes. The differentially expressed genes related to stem elongation were found to be primarily enriched in the diterpene biosynthesis pathway. Real-time fluorescence PCR validation showed that the relative expression levels of GA3ox gene in the shoot tips of Tianxiangqing and Gaoshanqing treated with gibberellin were 55.4% and 69.6% lower than those in the CK, respectively. The relative expression levels of GA20ox and KAO genes in Gaoshanqing asparagus lettuce with good stem elongation ability were higher than those in Tianxiangqing asparagus lettuce with weak stem elongation ability. In summary, exogenous gibberellin can affect the expression of key genes in the endogenous gibberellin synthesis pathway of asparagus lettuce. The stem elongation ability of 3-green asparagus lettuce is associated with the expression of LsGA20ox1 (a critical gene involved in endogenous gibberellin synthesis) and the transcription factor genes related to gibberellin metabolism.

参考文献/References:

[1]ACHARD P, GENSCHIK P. Releasing the brakes of plant growth:how GAs shutdown DELLA proteins[J]. Journal of Experimental Botany,2009,60(4):1085-1092.
[2]MAGOME H, YAMAGUCHI S, HANADA A ,et al. Dwarf and delayed-flowering 1,a novel Arabidopsis mutant deficient in gibberellin biosynthesis because of overexpression of a putative AP2 transcription factor[J]. The Plant Journal,2004,37(5):720-729.
[3]KUCHLAN P, KUCHLAN M K, HUSAIN S M. Effect of foliar application of growth activator,promoter and antioxidant on seed quality of soybean[J]. Legume Research,2016,40(2):313-318.
[4] KAZEMI M. Effect of gibberellic acid and potassium nitrate spray on vegetative growth and reproductive characteristics of tomato[J]. Journal of Biological and Environmental Sciences,2014,8(22):1-9.
[5]MICELI A, MONCADA A, SABATINO L, et al. Effect of gibberellic acid on growth,yield,and quality of leaf lettuce and rocket grown in a floating system[J]. Agronomy,2019,9(7):382.
[6]王力明,张泽锦,雷晓葵,等. 不同生长调节剂组合对“三青” 莴笋生长和光合作用的影响[J]. 北方园艺,2021(4):46-50.
[7]HEDDEN P. Gibberellin metabolism and its regulation[J]. Journal of Plant Growth Regulation,2001,20(4):317-318.
[8]CHIANG H H, HWANG I, GOODMAN H M. Isolation of the Arabidopsis GA4 locus[J]. The Plant Cell,1995,7(2):195-201.
[9]PHILLIPS A L, WARD D A, UKNES S, et al. Isolation and expression of three gibberellin 20-oxidase cDNA clones from Arabidopsis[J]. Plant Physiology,1995,108(3):1049-1057.
[10]QUE F, KHADR A, WANG G L, et al. Exogenous brassinosteroids altered cell length,gibberellin content,and cellulose deposition in promoting carrot petiole elongation[J]. Plant Science,2018,277:110-120.
[11]SONG Q L, CHENG S Y, CHEN Z X, et al. Comparative transcriptome analysis revealing the potential mechanism of seed germination stimulated by exogenous gibberellin in Fraxinus hupehensis[J]. BMC Plant Biology,2019,19(1):199.
[12]温玥. 外施赤霉素和多效唑对油茶花芽形成和果实品质的影响[D]. 北京:北京林业大学,2019.
[13]颜廷雨,成思丽,胡兆柳,等. 外源赤霉素对去顶后云南松生物量分配的影响及异速生长分析[J]. 林业科技通讯,2023(2):19-24.
[14]SUGIURA D, SAWAKAMI K, KOJIMA M, et al. Roles of gibberellins and cytokinins in regulation of morphological and physiological traits in Polygonum cuspidatum responding to light and nitrogen availabilities[J]. Functional Plant Biology,2015,42:397-409.
[15]NIEUWENHUIZEN N J, CHEN X Y, WANG M Y, et al. Natural variation in monoterpene synthesis in kiwifruit:transcriptional regulation of terpene synthases by NAC and ETHYLENE-INSENSITIVE3-like transcription factors[J]. Plant Physiology,2015,167(4):1243-1258.
[16]李欣,李影,曲子越,等. bHLH转录因子在茉莉酸信号诱导植物次生产物合成中的作用及分子机制[J]. 植物生理学报,2017,53(1):1-8.
[17]张凯伦,罗祖良,郭玉华,等. bHLH转录因子调控药用植物萜类化合物生物合成的研究进展[J]. 中国现代中药,2017,19(1):142-147.
[18]YAMAMURA C, MIZUTANI E, OKADA K, et al. Diterpenoid phytoalexin factor,a bHLH transcription factor,plays a central role in the biosynthesis of diterpenoid phytoalexins in rice[J]. The Plant Journal,2015,84(6):1100-1113.
[19]DESGAGN-PENIX I, SPONSEL V M. Expression of gibberellin 20-oxidase1 (AtGA20ox1) in Arabidopsis seedlings with altered auxin status is regulated at multiple levels[J]. Journal of Experimental Botany,2008,59(8):2057-2070.
[20]SAKAMOTO T, KOBAYASHI M, ITOH H, et al. Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice[J]. Plant Physiology,2001,125(3):1508-1516.
[21]SASAKI A, ITOH H, GOMI K, et al. Accumulation of phosphorylated repressor for gibberellin signaling in an F-box mutant[J]. Science,2003,299(5614):1896-1898.
[22]INTHIMA P, NAKANO M, OTANI M, et al. Overexpression of the gibberellin 20-oxidase gene from Torenia fournieri resulted in modified trichome formation and terpenoid metabolities of Artemisia annua L.[J]. Plant Cell,Tissue and Organ Culture,2017,129(2):223-236.
[23]ZHANG S W, ZHANG D, FAN S, et al. Effect of exogenous GA3 and its inhibitor paclobutrazol on floral formation,endogenous hormones,and flowering-associated genes in ‘Fuji’ apple (Malus domestica Borkh. )[J]. Plant Physiology and Biochemistry,2016,107:178-186.

备注/Memo

备注/Memo:
收稿日期:2024-12-09基金项目:四川省自然科学基金项目(2023NSFSC0159)；四川省“十四五”蔬菜育种攻关项目(2021YFYZ0022)作者简介:张泽锦(1984-),男,四川成都人,博士,副研究员,主要研究方向为蔬菜栽培技术研究。(E-mail)zhangzj127@163.com通讯作者:唐丽,(E-mail)tangli-999@163.com
更新日期/Last Update: 2025-10-27