[1]赵嘉庆,陈龙,苏娜,等.春尺蠖保幼激素酯酶基因AcinJHE生物信息学与表达分析[J].江苏农业学报,2025,(09):1721-1728.[doi:doi:10.3969/j.issn.1000-4440.2025.09.007]
　ZHAO Jiaqing,CHEN Long,SU Na,et al.Bioinformatics and expression analysis of juvenile hormone esterase gene AcinJHE from Apocheima cinerarius[J].,2025,(09):1721-1728.[doi:doi:10.3969/j.issn.1000-4440.2025.09.007]
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春尺蠖保幼激素酯酶基因AcinJHE生物信息学与表达分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1721-1728
栏目:
植物保护
出版日期:
2025-09-30

文章信息/Info

Title:
Bioinformatics and expression analysis of juvenile hormone esterase gene AcinJHE from Apocheima cinerarius
作者:
赵嘉庆1; 2; 3; 陈龙1; 2; 3; 苏娜4; 陈凯玥4
(1.河套学院农学系,内蒙古巴彦淖尔015000；2.河套地区绿色农产品安全生产与预警控制实验室,内蒙古巴彦淖尔015000；3.河套绿色农畜产品生产现代产业学院,内蒙古巴彦淖尔015000；4.内蒙古自治区林业和草原有害生物防治检疫总站,内蒙古呼和浩特010051)
Author(s):
ZHAO Jiaqing1; 2; 3; CHEN Long1; 2; 3; SU Na4; CHEN Kaiyue4
(1.Department of Agronomy, Hetao College, Bayannur 015000, China；2.Hetao Regional Laboratory for Safe Production and Early Warning & Control of Green Agricultural Products, Bayannur 015000, China；3.Hetao Modern Industry Institute of Green Agricultural and Livestock Products Production, Bayannur 015000, China；4.Inner Mongolia Autonomous Region General Station of Forestry and Grassland Pest Control and Quarantine, Hohhot 010051, China)
关键词:
春尺蠖; 保幼激素酯酶基因（JHE）; 低温胁迫; 饥饿胁迫
Keywords:
Apocheima cinerarius; juvenile hormone esterase (JHE); low temperature stress; starvation stress
分类号:
S888.72+1
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.007
文献标志码:
A
摘要:
为探究保幼激素酯酶(JHE)在春尺蠖(Apocheima cinerarius)生长发育及环境适应中的作用,本研究基于春尺蠖转录组数据,通过同源比对筛选并鉴定到1个JHE基因,命名为AcinJHE。该基因的CDS序列全长1 755 bp,编码的蛋白质包含590个氨基酸,预测蛋白质相对分子量为65 580,等电点为5.70,含有1条长度25 aa的信号肽,无跨膜结构,其羧酸酯酶保守结构域位于39～579 aa。系统进化分析结果表明,AcinJHE与冬尺蠖JHE亲缘关系最近,聚类于同一分支。从幼虫至非滞育蛹阶段,AcinJHE基因的相对表达量显著升高(P＜0.05)。从滞育蛹至滞育解除蛹阶段,春尺蠖AcinJHE基因的相对表达量显著降低(P＜0.05)。AcinJHE基因在雄成虫头中的相对表达量最高。AcinJHE基因响应低温和饥饿胁迫。4 ℃低温处理3 h后,春尺蠖3龄幼虫AcinJHE基因的相对表达量达到峰值,饥饿处理72 h,春尺蠖4龄幼虫AcinJHE基因的相对表达量显著高于对照(P＜0.05)。本研究解析了AcinJHE基因在调控春尺蠖生长发育和逆境适应中的分子机制,为防治春尺蠖的危害提供了理论依据。
Abstract:
To investigate the role of juvenile hormone esterase (JHE) in the growth, development, and environmental adaptation of Apocheima cinerarius, this study screened and identified a JHE gene based on the transcriptomic data of A. cinerarius via homologous alignment, and named it AcinJHE. The CDS sequence of this gene was 1 755 bp in length, encoding a protein composed of 590 amino acids. The predicted relative molecular weight of the protein was 65 580, with an isoelectric point of 5.70. It contained a signal peptide of 25 aa in length, lacked transmembrane structures, and its carboxylesterase conserved domain was located at 39-579 aa. Phylogenetic analysis results showed that AcinJHE had the closest genetic relationship with the JHE of Operophtera brumata, clustering on the same branch. From the larval stage to the non-diapause pupal stage, the relative expression level of AcinJHE increased significantly(P＜0.05). From the diapause pupal stage to the diapause-terminated pupal stage, the relative expression level of AcinJHE in A. cinerarius decreased significantly(P＜0.05). The relative expression level of AcinJHE was the highest in the heads of male adults. The AcinJHE gene responded to low-temperature and starvation stresses. After three hours of low-temperature treatment at 4 ℃, the relative expression level of the AcinJHE gene in the 3rd-instar larvae of A. cinerarius reached the peak. After 72 hours of starvation treatment, the relative expression level of the AcinJHE gene in the 4th-instar larvae of A. cinerarius was significantly higher than that in the control group (P＜0.05). This study elucidates the molecular mechanism of the AcinJHE gene in regulating the growth, development, and stress adaptation of A. cinerarius, thereby providing a theoretical basis for its control.

参考文献/References:

[1]王雨,斯琴,王秀梅,等. 天然胡杨林春尺蠖飞防存在的问题及解决措施[J]. 北京林业大学学报,2024,46(4):113-119.
[2]庞竟公,何稳稳,曹玉,等. 重要林木害虫春尺蠖研究进展[J]. 农业科学,2018,8(5):461-467.
[3]DING W C, LI H Y, WEN J B. Climate change impacts on the potential distribution of Apocheima cinerarius (Erschoff) (Lepidoptera:Geometridae)[J]. Insects,2022,13:59.
[4]卿薇,阿地力·沙塔尔,闫文兵. 春尺蠖生物学特性研究[J]. 应用昆虫学报,2016,53(1):174-184.
[5]陈龙,娜仁格日乐,戴桂香,等. 春尺蠖海藻糖转运蛋白基因AcinTret1和AcinTret1-like的克隆、分子特性与表达分析[J]. 江苏农业科学,2022,50 (10):23-30.
[6]AYOADE O, MOROOKA S, TOJO S. Enhancement of short wing formation and ovarian growth in the genetically defined macropterous strain of the brown planthopper,Nilaparvata lugens[J]. Journal of Insect Physiology,1999,45(1):93-100.
[7]ZHAO J, ZHOU Y L, LI X, et al. Silencing of juvenile hormone epoxide hydrolase gene (Nljheh) enhances short wing formation in a macropterous strain of the brown planthopper,Nilaparvata lugens [J]. Journal of Insect Physiology,2017,102:18-26.
[8]RIDDIFORD L M, TRUMAN J W, MIRTH C K, et al. A role for juvenile hormone in the prepupal development of Drosophila melanogaster [J]. Development,2010,137(7):1117-1126.
[9]JINDRA M, PALLI S R, RIDDIFORD L M. The juvenile hormone signaling pathway in insect development [J]. Annual Review of Entomology,2013,58:181-204.
[10]RIDDIFORD L M. How does juvenile hormone control insect metamorphosis and reproduction?[J]. General and Comparative Endocrinology,2012,179(3):477-484.
[11]ROY S, SAHA T T, ZOU Z, et al. Regulatory pathways controlling female insect reproduction[J]. Annual Review of Entomology,2018,63:489-511.
[12]KAMITA S G, HAMMOCK B D. Juvenile hormone esterase:biochemistry and structure[J]. Journal of Pesticide Science,2010,35(3):265-274.
[13]NIU D, ZHENG H C, CORONA M, et al. Transcriptome comparison between inactivated and activated ovaries of the honey bee Apis mellifera L.[J]. Insect Biochemistry and Molecular Biology,2014,23(5):668-681.
[14]VERA J C, WHEAT C W, FESCEMYER H W, et al. Rapid transcriptome characterization for a nonmodel organism using 454 pyrosequencing[J]. Molecular Ecology,2008,17(7):1636-1647.
[15]LIU S H, YANG B J, GU J H, et al. Molecular cloning and characterization of a juvenile hormone esterase gene from brown planthopper,Nilaparvata lugens[J]. Journal of Insect Physiology,2008,54(12):1495-1502.
[16]CHOI J H, KIJIMOTO T, SNELL-ROOD E, et al. Gene discovery in the homed beetle Onthophagus taurus[J]. BMC Genomics,2010,11(1):1-19.
[17]CHEN E H, WEI D D, SHEN G M, et al. De novo characterization of the Dialeurodes citri transcriptome:mining genes involved in stress resistance and simple sequence repeats (SSRs) discovery[J]. Insect Molecular Biology,2014,23:52-66.
[18]齐晓阳. 七星瓢虫比较转录组测序及滞育关联基因的克隆与初步分析[D]. 福州:福建农林大学,2016.
[19]LI H C, ZHANG H, GUAN R B, et al. Identification of differential expression genes associated with host selection and adaptation between two sibling insect species by transcriptional profile analysis[J]. BMC Genomics,2013,14(14):489-490.
[20]HAMMOCK B D, BONNING B C, POSSEE R D, et al. Expression and effects of the juvenile hormone esterase in a baculovirus vector[J]. Nature,1990,344(6265):458-461.
[21]LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method[J]. Methods,2001,25(4):402-408.
[22]ZHU L, YIN T Y, SUN D, et al. Juvenile hormone regulates the differential expression of putative juvenile hormone esterases via methoprene-tolerant in non-diapausedestined and diapause-destined adult female beetle[J]. Gene,2017,627:373-378.
[23]WARD V K, BONNING B C, HUANG T, et al. Analysis of the catalytic mechanism of juvenile hormone esterase by site-directed mutagenesis[J]. The International Journal of Biochemistry,1992,24(12):1933-1941.
[24]王晶晶,梁婷婷,成卫宁,等. 小麦吸浆虫保幼激素酯酶和保幼激素环氧水解酶基因的克隆及在滞育和变态发育过程中的表达动态[J]. 昆虫学报,2020,63(4):401-412.
[25]龙楚云,郭建洋,万方浩. 烟粉虱 MED 隐种保幼激素酯酶 cDNA片段克隆、序列分析及在不同发育阶段的表达[J]. 昆虫学报,2013,56(3):234-242.
[26]MACKERT A, DO NASCIMENTO A M, BITONDI M M G, et al. Identification of a juvenile hormone esterase-like gene in the honey bee,Apis mellifera L.-Expression analysis and functional assays[J]. Comparative Biochemistry and Physiology Part B:Biochemistry and Molecular Biology,2008,150(1):33-44.
[27]闫丽琼,汤方,龚尚骞,等. 舞毒蛾保幼激素结合蛋白家族基因克隆及对CO2胁迫的响应[J]. 环境昆虫学报,2019,41(6):1339-1347.
[28]朱圣杰,崔阔澍,白松林,等. 春尺蠖保幼激素结合蛋白AcinJHBP基因的克隆、分子特征与表达分析[J]. 西南农业学报,2024,37(7):1538-1544.
[29]CHENG W N, LI X J, ZHAO J J, et al. Cloning and characterization of methoprene-tolerant (Met) and Krüppel homolog1 (Kr-h1) genes in the wheat blossom midge,Sitodiplosis mosellana[J]. Insect Science,2020,27(2):292-303.
[30]KADONO-OKUDA K,戴玉锦. 对家蚕饥饿幼虫应用保幼激素类似物诱导完好超龄幼虫[J]. 国外农学——蚕业,1988(4):23-28.
[31]HIDAYAT P, ASHARI F, WULANDARI S. Starvation-induced upregulation of juvenile hormone esterase mediates precocious metamorphosis in Spodoptera litura[J]. Insect Biochemistry and Molecular Biology,2021,135:103602.
[32]周红艳. 温度和激素对柑橘大实蝇发育的影响[D]. 重庆:西南大学,2013
[33]王宗舜. 昆虫滞育与激素调节[J]. 昆虫知识,1985(4):40-42.
[34]李玉艳,张礼生,陈红印. 茧蜂滞育的研究进展[J]. 昆虫学报,2010,53(10):1167-1178.
[35]LAI R Q, BAI J J, GU G, et al. Juveniles on aphids to prolong optimal stages[J]. Entomological Research,2021,51(3):133-142.
[36]MACMILLAN H A, FERGUSON L V, NICOLAI A, et al. Parallel ionoregulatory adjustments underlie phenotypic plasticity and evolution of Drosophila cold tolerance[J]. Journal of Experimental Biology,2016,219(7):980-994.
[37]MACMILLAN H A, SINCLAIR B J. Mechanisms underlying insect chill-coma[J]. Journal of Insect Physiology,2011,57(1):12-20.
[38]DENLINGER D L, YOCUM G D, RINEHART J P. Hormonal control of diapause[J]. Insect Endocrinology,2012,8:430-463.

备注/Memo

备注/Memo:
收稿日期：2025-06-13基金项目：内蒙古自然科学基金项目(2025LHMS03067、2021BS03028)；“科技兴蒙”行动重点专项(NMKJXM202201)；巴彦淖尔市科技计划项目(NMKJXM202408)作者简介：赵嘉庆(2004-),女,内蒙古乌兰察布人,本科,主要从事昆虫生态与分子生物学研究。(E-mail)2292345480@qq.com通讯作者：陈龙,(E-mail)15774711140@163.com
更新日期/Last Update: 2025-10-27