[1]段玉玲,朱小雨,郝婷婷.原卟啉原Ⅸ氧化酶基因PPO2过表达赋予水稻对丙炔氟草胺除草剂的耐受性[J].江苏农业学报,2025,(09):1674-1682.[doi:doi:10.3969/j.issn.1000-4440.2025.09.002]
　DUAN Yuling,ZHU Xiaoyu,HAO Tingting.Overexpression of protoporphyrinogen Ⅸ oxidase gene PPO2 in rice confers tolerance to the herbicide flumioxazin[J].,2025,(09):1674-1682.[doi:doi:10.3969/j.issn.1000-4440.2025.09.002]
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原卟啉原Ⅸ氧化酶基因PPO2过表达赋予水稻对丙炔氟草胺除草剂的耐受性()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1674-1682
栏目:
遗传育种·生理生化
出版日期:
2025-09-30

文章信息/Info

Title:
Overexpression of protoporphyrinogen Ⅸ oxidase gene PPO2 in rice confers tolerance to the herbicide flumioxazin
作者:
段玉玲1; 朱小雨1; 郝婷婷2
(1.扬州大学生物科学与技术学院,江苏扬州225009；2.江苏省农业科学院种质资源与生物技术研究所,江苏南京210014)
Author(s):
DUAN Yuling1; ZHU Xiaoyu1; HAO Tingting2
(1.College of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China;2.Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
水稻; OsPPO2; 过表达; 除草剂抗性
Keywords:
rice; OsPPO2; overexpression; herbicide resistance
分类号:
S482.4;S511
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.002
文献标志码:
A
摘要:
近年来,稻田抗性杂草的发生日益加剧,培育抗除草剂水稻新品种,是高效解决抗性杂草问题的措施之一。原卟啉原Ⅸ氧化酶(PPO)是植物生长过程中叶绿素和血红素合成的关键酶之一。据报道,植物中PPO存在2种异构体,即质体靶向（PPO1）和线粒体靶向（PPO2）。本研究以PPO2基因为研究对象,通过构建表达载体pCAMBIA1309-OsPPO2,利用农杆菌介导法将来自镇稻18的PPO2基因导入秀水134 (作为野生型,即WT)中。通过筛选和鉴定,成功获得了过表达OsPPO2的转基因株系OsPPO2-OE。通过除草剂封闭喷施和茎叶喷雾处理,检测WT和OsPPO2-OE对丙炔氟草胺的抗性。封闭喷施处理结果表明,过表达OsPPO2的水稻株系比WT对丙炔氟草胺有更好的耐受性,约为WT的2倍。茎叶喷雾处理结果显示,在低浓度除草剂条件下,WT生长受到了明显抑制,植株矮化,而过表达OsPPO2株系表现出更好的耐受性。双逻辑非线性回归模型分析结果表明,在丙炔氟草胺处理下,WT和过表达OsPPO2-OE株系的鲜重抑制中量(GR50)分别为43.17 g/hm2,a.i.和88.25 g/hm2,a.i.,抗性指数(RI)均为2.04。此外,叶绿素含量测定结果显示,转基因株系与WT相比没有显著差异。本研究结果证明了水稻内源基因OsPPO2在提高水稻耐PPO类除草剂方面的可行性,并为未来培育耐除草剂水稻新品种提供了重要的理论依据和实践参考。
Abstract:
In recent years, the occurrence of resistant weeds in paddy fields has been increasing. Breeding new varieties of herbicide-resistant rice is one of the effective measures to solve the problem of resistant weeds. Protoporphyrinogen Ⅸ oxidase (PPO) is one of the key enzymes in the synthesis of chlorophyll and heme in plant growth. It was reported that there were two isomers of PPO in plants, namely plastid targeted PPO1 and mitochondrial targeted PPO2. In this study, the PPO2 gene was taken as the research object. An expression vector pCAMBIA1309-OsPPO2 was constructed, and the PPO2 gene from Zhendao 18 was introduced into Xiushui 134 (WT) by Agrobacterium-mediated transformation. Through screening and identification, transgenic lines overexpressing OsPPO2 (OsPPO2-OE) were successfully obtained. The resistance of WT and OsPPO2-OE lines to flumioxazin was evaluated through herbicide application via soil pre-emergence (closed spray) and foliar post-emergence treatments. The results of pre-emergence treatment showed that the rice lines overexpressing OsPPO2 had better tolerance to flumioxazin than WT, the tolerance was about twice that of WT. The results of post-emergence treatment showed that under low herbicide concentration conditions, the growth of WT was significantly inhibited, with dwarfing of plants, while the transgenic lines overexpressing OsPPO2 exhibited better tolerance. The results of double logistic nonlinear regression model analysis showed that under flumioxazin treatment, the 50% fresh weight reduction (GR50) values for WT and the transgenic line overexpressing OsPPO2-OE were 43.17 g/hm2, a.i. and 88.25 g/hm2, a.i., respectively, with a resistance index (RI) of 2.04. In addition, the chlorophyll content determination results showed no significant difference between the transgenic lines and WT. This study has demonstrated the feasibility of improving rice tolerance to PPO herbicides by overexpressing the endogenous OsPPO2 gene in rice and has provided important theoretical basis and practical reference for the future development of herbicide-tolerant rice varieties.

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备注/Memo

备注/Memo:
收稿日期：2025-03-07基金项目：农业生物育种国家科技重大专项(2023ZD0406602)；中央引导地方科技发展资金农业科技项目(BE2024001-2)作者简介：段玉玲(1998-),女，四川内江人,硕士研究生,主要从事水稻抗除草剂育种研究。(E-mail)1121917346@qq.com通讯作者：郝婷婷,(E-mail)17812067912@126.com
更新日期/Last Update: 2025-10-27