[1]王歆凯,李阳,凌溪铁,等.水稻抗甲咪唑烟酸突变体对多种ALS抑制剂类除草剂的抗性分析[J].江苏农业学报,2025,(09):1665-1673.[doi:doi:10.3969/j.issn.1000-4440.2025.09.001]
　WANG Xinkai,LI Yang,LING Xitie,et al.Resistance analysis of imazapic-tolerant rice mutants to various ALS-inhibiting herbicides[J].,2025,(09):1665-1673.[doi:doi:10.3969/j.issn.1000-4440.2025.09.001]
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水稻抗甲咪唑烟酸突变体对多种ALS抑制剂类除草剂的抗性分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年09期
页码:
1665-1673
栏目:
遗传育种·生理生化
出版日期:
2025-09-30

文章信息/Info

Title:
Resistance analysis of imazapic-tolerant rice mutants to various ALS-inhibiting herbicides
作者:
王歆凯1; 李阳1; 2; 凌溪铁1; 裘实1; 张保龙1; 2; 3; 王金彦2; 3
(1.海南大学热带农林学院,海南海口570228;2.江苏省农业科学院种质资源与生物技术研究所/江苏省农业生物学重点实验室,江苏南京210014；3.江苏大学食品与生物工程学院,江苏镇江212013)
Author(s):
WANG Xinkai1; LI Yang1; 2; LING Xitie1; QIU Shi1; ZHANG Baolong1; 2; 3; WANG Jinyan2; 3
(1.School of Tropical Agriculture and Forestry, Hainan University, Haikou 570228, China;2.Institute of Germplasm Resources and Biotechnology, Jiangsu Academy of Agricultural Sciences/Provincial Key Laboratory of Agrobiology, Nanjing 210014, China;3.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China)
关键词:
ALS抑制剂类除草剂; 水稻突变体; 咪唑啉酮类除草剂; 磺酰脲类除草剂; 乙酰乳酸合成酶（ALS）
Keywords:
ALS-inhibiting herbicides; rice mutant; imidazolinone herbicides; sulfonylurea herbicides; acetolactate synthase (ALS)
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2025.09.001
文献标志码:
A
摘要:
为创制抗乙酰乳酸合成酶(ALS)抑制剂类除草剂水稻种质、促进ALS抑制剂类除草剂在水稻生产中的应用,本研究以连粳15为试验材料,利用甲磺酸乙酯(Ethyl methanesulfonate,EMS)诱变和甲咪唑烟酸除草剂喷施处理,筛选获得抗甲咪唑烟酸的水稻突变体,通过遗传分析和基因克隆明确突变体的突变位点及类型,并对野生型植株和突变体植株进行喷施无菌水（CK）和5种常用的ALS抑制剂类除草剂(甲氧咪草烟、甲咪唑烟酸、咪唑乙烟酸、甲基二磺隆和烟嘧磺隆)处理,分析突变体的抗性水平。结果表明,共筛选获得1个抗甲咪唑烟酸的水稻突变体,突变体ALS基因核苷酸序列在第1 880位的碱基由G突变为碱基A，ALS蛋白氨基酸序列中第627个氨基酸由丝氨酸突变为天冬酰胺。喷施咪唑啉酮类除草剂甲氧咪草烟、甲咪唑烟酸和咪唑乙烟酸处理,野生型植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量均显著低于喷施无菌水对照(CK),突变体植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量与CK无显著差异。喷施磺酰脲类除草剂甲基二磺隆和烟嘧磺隆处理,野生型植株和突变体植株ALS活性、茎叶鲜重和根系鲜重、叶片叶绿素总含量均显著低于CK。因此,本研究获得的突变体对咪唑啉酮类除草剂具有较强的抗性,但对磺酰脲类除草剂抗性相对较差。
Abstract:
To create rice germplasm resistant to acetolactate synthase (ALS) inhibitor herbicides and promote the application of ALS inhibitor herbicides in rice production, ethyl methanesulfonate (EMS) mutagenesis and imazapic herbicide spraying treatment were used to screen rice mutants resistant to imazapic with Lianjing 15 as the experimental material in this study. The mutation sites and types of the mutants were determined by genetic analysis and gene cloning. The wild-type and mutant plants were sprayed with sterile water (control, CK) and five commonly used ALS inhibitor herbicides (imazamox, imazapic, imazethapyr, mesosulfuron-methyl and nicosulfuron) to analyze the resistance level of the mutant. The results showed that one rice mutant resistant to imazapic was obtained. A nucleotide substitution from G to A was identified at position 1 880 in the ALS gene, resulting in a serine-to-asparagine change at the 627th amino acid residue in the ALS protein. The ALS activity, stem and leaf fresh weight, root fresh weight and total chlorophyll content of wild-type plants were significantly lower than those in the sterile water control (CK) after spraying imidazolinone herbicides imazamox， imazapic and imazethapyr. In contrast, the mutant plants showed no significant differences in these parameters compared to the CK. The ALS activity, stem and leaf fresh weight, root fresh weight and total chlorophyll content of wild-type and mutant plants were significantly lower than those of CK after spraying sulfonylurea herbicides mesosulfuron-methyl and nicosulfuron. Therefore, the mutants obtained in this study exhibit strong resistance to imidazolinone herbicides but weak resistance to sulfonylurea herbicides.

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相似文献/References:

[1]周佳伟,王歆凯,唐兆成,等.EMS诱变的水稻不同位点突变体对ALS抑制剂类除草剂抗性差异[J].江苏农业学报,2024,(07):1153.[doi:doi:10.3969/j.issn.1000-4440.2024.07.001]
　ZHOU Jiawei,WANG Xinkai,TANG Zhaocheng,et al.Differences in ALS inhibitor herbicide resistance among rice mutants with different loci induced by EMS mutagenesis[J].,2024,(09):1153.[doi:doi:10.3969/j.issn.1000-4440.2024.07.001]

备注/Memo

备注/Memo:
收稿日期：2025-04-19基金项目：江苏省农业科技自主创新基金项目[CX(19)3054]作者简介：王歆凯(1999-),男,山西长治人,硕士研究生,主要从事水稻抗除草剂育种研究。(E-mail)13363457576@163.com通讯作者：张保龙(E-mail)zhbl2248@hotmail.com
更新日期/Last Update: 2025-10-27