[1]潘帆,孙振南,李文雅,等.香鱼Foxl2基因克隆及甲基睾酮对其表达的影响[J].江苏农业学报,2025,(08):1583-1591.[doi:doi:10.3969/j.issn.1000-4440.2025.08.015]
　PAN Fan,SUN Zhennan,LI Wenya,et al.Cloning of Foxl2 gene and the influence of methyltestosterone on its expression in sweetfish Plecoglossus altivelis[J].,2025,(08):1583-1591.[doi:doi:10.3969/j.issn.1000-4440.2025.08.015]
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香鱼Foxl2基因克隆及甲基睾酮对其表达的影响()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1583-1591
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-08-31

文章信息/Info

Title:
Cloning of Foxl2 gene and the influence of methyltestosterone on its expression in sweetfish Plecoglossus altivelis
作者:
潘帆1; 2; 孙振南1; 2; 李文雅1; 2; 王宏艳1; 2; 邵鑫斌3; 马建忠3; 李明云1; 2; 苗亮1; 2
(1.宁波大学农产品质量安全危害因子与风险防控国家重点实验室,浙江宁波315211;2.宁波大学水产生物技术教育部重点实验室,浙江宁波315832;3.浙江省海洋水产养殖研究所,浙江温州325005)
Author(s):
PAN Fan1; 2; SUN Zhennan1; 2; LI Wenya1; 2; WANG Hongyan1; 2; SHAO Xinbin3; MA Jianzhong3; LI Mingyun1; 2; MIAO Liang1; 2
(1.State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products， Ningbo University, Ningbo 315211, China;2.Key Laboratory of Aquacultural Biotechnology of Ministry of Education, Ningbo University, Ningbo 315832, China;3.Zhejiang Mariculture Research Institute, Wenzhou 325005, China)
关键词:
香鱼; Foxl2; 甲基睾酮; 基因表达
Keywords:
Plecoglossus altivelis; Foxl2; methyltestosterone; gene expression
分类号:
Q958.8
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.015
文献标志码:
A
摘要:
为探究香鱼性腺发育及生殖机能调控机制,本研究拟克隆香鱼 Foxl2 (Forkhead transcription factor gene 2)基因,检测成鱼不同组织中Foxl2的表达情况,分析腹腔注射不同质量浓度(15 μg/μL、75 μg/μL、150 μg/μL)甲基睾酮（MT）对香鱼脑和性腺中Foxl2基因表达水平的影响。结果表明:香鱼 Foxl2 基因开放阅读框(ORF)碱基序列长度为918 bp,编码蛋白质由305个氨基酸组成，含典型的叉头（Forkhead）结构域；多重序列比对结果显示，各种脊椎动物间Foxl2蛋白保守性较高,特别是Forkhead结构域高度保守；系统进化树中香鱼 Foxl2蛋白与虹鳟(Oncorhynchus mykiss)Foxl2蛋白、大西洋鲑(Salmo salar) Foxl2蛋白优先聚为1支,说明它们进化关系较近。 Foxl2 基因在香鱼卵巢中高表达,雌鱼与雄鱼的脑和鳃中较低表达,精巢及其他组织中无表达。腹腔注射甲基睾酮后1 d,不同质量浓度处理雌鱼卵巢中Foxl2基因相对表达量显著上调，但脑中Foxl2基因相对表达量无显著变化,且卵巢中Foxl2基因相对表达量增加幅度与MT质量浓度呈负相关,15 μg/μL和75 μg/μL质量浓度处理雄鱼脑中Foxl2基因相对表达量显著升高,精巢中Foxl2基因始终无表达。上述结果说明,Foxl2在香鱼性腺中呈性别二态性表达,外源性激素显著影响香鱼卵巢中Foxl2基因相对表达量。本研究结果为探究Foxl2基因功能及香鱼性别调控机制提供了基础。
Abstract:
In order to explore the regulation mechanism of gonadal development and reproductive function in Plecoglossus altivelis, the Foxl2 (Forkhead transcription factor gene 2) gene of Plecoglossus altivelis was to be cloned in this study, and the expression of Foxl2 gene in different tissues of adult fish was targeted for detecting. The effects of intraperitoneal injection of different mass concentrations (15 μg/μL, 75 μg/μL, 150 μg/μL) of methyltestosterone (MT) on the expression level of Foxl2 gene in brain and gonad of Plecoglossus altivelis were to be analyzed. The results showed that the open reading frame (ORF) of Foxl2 gene was 918 bp in length, and the protein encoded was composed of 305 amino acids and contained a typical Forkhead domain. Multiple sequence alignment showed that Foxl2 proteins were highly conserved among various vertebrates, the Forkhead domain was exceptionally highly conserved. In the phylogenetic tree, Plecoglossus altivelis Foxl2 protein was preferentially clustered with rainbow trout (Oncorhynchus mykiss) Foxl2 protein and Atlantic salmon (Salmo salar) Foxl2 proteins, indicating that they had close evolutionary relationships. Foxl2 gene was highly expressed in the ovary of Plecoglossus altivelis, and was lowly expressed in the brain and gill of female and male fish, but was not expressed in testis or other tissues. One day after intraperitoneal injection of methyltestosterone, the relative expression of Foxl2 gene in ovary of female fish treated with different mass concentrations was significantly up-regulated, but there was no significant change in the relative expression of Foxl2 gene in brain. The increase of relative expression of Foxl2 gene in ovary was negatively correlated with MT mass concentration. The relative expression of Foxl2 gene in brain of male fish treated with 15μg/μL and 75μg/μL was significantly increased, while Foxl2 gene in testis was always not expressed. The above results indicate that Foxl2 gene is expressed in sexual dimorphism in the gonads of Plecoglossus altivelis, and exogenous hormones significantly affect the relative expression of Foxl2 gene in the ovary of Plecoglossus altivelis. The results of this study provide a basis for exploring the function of Foxl2 gene and the sex regulation mechanism of Plecoglossus altivelis.

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备注/Memo

备注/Memo:
收稿日期:2025-01-17基金项目:浙江省基础公益研究计划项目(LGN20C190009)；中央引导地方科技发展资金项目(2023ZY1056)作者简介:潘帆(2000-),女,安徽池州人,硕士研究生,主要研究方向为水产养殖。(E-mail)1564105203@qq.com通讯作者:苗亮,(E-mail)miaoliang@nbu.edu.cn
更新日期/Last Update: 2025-09-23