[1]王倩倩,赵永,杨齐心,等.柯乐猪BMP7基因SNP位点鉴定及其与繁殖性状的关联分析[J].江苏农业学报,2025,(08):1559-1565.[doi:doi:10.3969/j.issn.1000-4440.2025.08.012]
　WANG Qianqian,ZHAO Yong,YANG Qixin,et al.Identification of SNP loci in the BMP7 gene of Kele pig and analysis of their association with reproductive traits[J].,2025,(08):1559-1565.[doi:doi:10.3969/j.issn.1000-4440.2025.08.012]
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柯乐猪BMP7基因SNP位点鉴定及其与繁殖性状的关联分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1559-1565
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-08-31

文章信息/Info

Title:
Identification of SNP loci in the BMP7 gene of Kele pig and analysis of their association with reproductive traits
作者:
王倩倩1; 赵永1; 杨齐心2; 杨红文2; 向进1; 吴燕1; 付红梅1; 丁义杰1; 张依裕1
(1.贵州大学动物科学学院高原山地动物遗传育种与繁殖教育部重点实验室/贵州省动物遗传育种与繁殖重点实验室/贵州大学香猪研究所,贵州贵阳550025；2.贵州省畜禽遗传资源管理站,贵州贵阳550001)
Author(s):
WANG Qianqian1; ZHAO Yong1; YANG Qixin2; YANG Hongwen2; XIANG Jin1; WU Yan1; FU Hongmei1; DING Yijie1; ZHANG Yiyu1
(1.Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region of Ministry of Education, College of Animal Science of Guizhou University/Guizhou Provincial Key Laboratory of Animal Genetics Breeding and Reproduction/Institute of Swine, Guizhou University, Guiyang 550025, China；2.Guizhou Provincial Poultry and Livestock Genetic Resources Management Station, Guiyang 550001, China)
关键词:
BMP7; 单核苷酸多态性位点; 繁殖性能; 柯乐猪; 关联分析
Keywords:
BMP7; single nucleotide polymorphism loci; reproductive performance; Kele pig; association analysis
分类号:
S828
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.012
文献标志码:
A
摘要:
为了探究BMP7基因单核苷酸多态性（SNP）位点对柯乐猪繁殖性状的影响,本研究选取158头健康经产柯乐猪,采用Sanger测序技术检测BMP7基因的SNP位点,并对其与繁殖性状的相关性进行分析。结果表明,在BMP7基因第3内含子区域鉴定到3个SNP突变位点,分别为g.57647887 G→A、g.57647990 C→T和g.57648145 C→G,这些位点均符合哈迪-温伯格平衡(P＞0.05)。其中,g.57647887 G→A位点与g.57647990 C→T位点完全连锁。关联分析结果表明,H1H1(GG-CC-GG)、H2H2(AA-TT-CC)和H4H4(AA-TT-GG)双倍型个体的总产仔数、产活仔猪数、初生窝重、断奶仔猪数、断奶窝重等繁殖性状较好,且为纯合子,而H2H4(AA-TT-GC)双倍型个体的繁殖性状较差。本研究结果为柯乐猪育种提供了理论依据。
Abstract:
To investigate the effects of BMP7 gene single nucleotide polymorphisms (SNPs) loci on reproductive traits in Kele pigs, this study selected 158 healthy multiparous Kele pigs and used Sanger sequencing technology to detect SNPs in the BMP7 gene. The correlations between these SNPs and reproductive traits were also analyzed. The results showed that three SNPs mutational sites were identified in the third intron of the BMP7 gene, namely g.57647887 G→A, g.57647990 C→T, and g.57648145 C→G, all of which were in Hardy-Weinberg equilibrium (P＞0.05). Among them, the g.57647887 G→A and g.57647990 C→T loci were in complete linkage disequilibrium. The association analysis indicated that Kele pigs with the H1H1 (GG-CC-GG), H2H2 (AA-TT-CC), and H4H4 (AA-TT-GG) diplotypes exhibited better reproductive traits, such as total number of piglets born, number of live piglets born, litter weight at birth, number of piglets weaned, and litter weight at weaning. These diplotypes were also homozygous. In contrast, Kele pigs with the H2H4 (AA-TT-GC) diplotype had poorer reproductive traits. This study results provide a theoretical basis for breeding Kele pigs.

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备注/Memo

备注/Memo:
收稿日期:2025-01-02基金项目:贵州省科技计划项目[黔科合平台人才(2021)5630号]；贵州省生猪产业技术体系建设项目(GZSZCYJSTX-03)；贵州省科技支撑计划项目[黔科合支撑(2021）一般 147]；贵州省生猪产业发展项目[黔财农(2021）157号、黔财农(2022)183号]；贵州省种业发展项目[黔农计财(2022)10号]；国家重点研发计划子课题项目(2022YFD1100308-01)作者简介:王倩倩(2002-),女,贵州毕节人,硕士研究生,研究方向为动物遗传育种与繁殖,(E-mail)2184500899@qq.com通讯作者:张依裕,(E-mail)zyy8yyc@163.com
更新日期/Last Update: 2025-09-23