[1]高永煌,俞咪娜,于俊杰,等.稻曲病病菌植物细胞壁降解酶活性测定及其与致病力的相关性分析[J].江苏农业学报,2025,(08):1490-1498.[doi:doi:10.3969/j.issn.1000-4440.2025.08.005]
　GAO Yonghuang,YU Mina,YU Junjie,et al.Determination of plant cell wall-degrading enzyme activity in Ustilaginoidea virens and analysis of its correlation with pathogenicity[J].,2025,(08):1490-1498.[doi:doi:10.3969/j.issn.1000-4440.2025.08.005]
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稻曲病病菌植物细胞壁降解酶活性测定及其与致病力的相关性分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1490-1498
栏目:
植物保护
出版日期:
2025-08-31

文章信息/Info

Title:
Determination of plant cell wall-degrading enzyme activity in Ustilaginoidea virens and analysis of its correlation with pathogenicity
作者:
高永煌1; 2; 俞咪娜2; 于俊杰2; 曹慧娟2; 宋天巧2; 潘夏艳2; 王淑琛2; 王云鹏1; 靳丛1; 刘永锋2
(1.淮阴工学院,江苏淮安223001；2.江苏省农业科学院植物保护研究所,江苏南京210014)
Author(s):
GAO Yonghuang1; 2; YU Mina2; YU Junjie2; CAO Huijuan2; SONG Tianqiao2; PAN Xiayan2; WANG Shuchen2; WANG Yunpeng1; JIN Cong1; LIU Yongfeng2
(1.Huaiyin Institute of Technology, Huai’an 223001, China；2.Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China)
关键词:
稻曲病; 植物细胞壁降解酶; 酶活性; 水稻组织
Keywords:
rice false smut; plant cell wall-degrading enzymes; enzyme activity; rice tissues
分类号:
S432.4+4
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.005
文献标志码:
A
摘要:
植物细胞壁降解酶(PCWDE)是病原菌的重要致病因子。为了明确植物细胞壁降解酶在稻曲病病菌侵染水稻过程中的作用,首先基于碳水化合物活性酶数据库(CAZy)成功鉴定了47个植物细胞壁降解酶编码基因。比较基因组学的分析结果表明,稻曲病病菌的植物细胞壁降解酶数量与其他活体营养型病原菌相似,显著少于半活体营养型病原菌、腐生型病原菌。转录组动态分析结果显示,稻曲病病菌中UV8b_01537、UV8b_03436和UV8b_04918等3个关键基因的相对表达量在接种水稻48 h后显著上调,其编码蛋白质分别参与纤维素、半纤维素和淀粉的降解。用对硝基苯酚显色底物检测2个具有强致病性菌株的酶活性发现,PS培养液、水稻叶组织培养液、穗组织培养液均能诱导病菌分别产生降解淀粉、纤维素、半纤维素的α-葡萄糖苷酶、β-葡萄糖苷酶和β-木聚糖酶；α-葡萄糖苷酶、β-葡萄糖苷酶和β-木聚糖酶的总酶活性随着培养时间的增加呈动态变化,且在28 ℃恒温摇床上于180 r/min振荡培养的第4 d出现峰值,与致病力呈负相关；用水稻穗组织培养液诱导的Nj7菌株的3种酶（α-葡萄糖苷酶、β-葡萄糖苷酶和β-木聚糖酶）的总活性显著低于用水稻叶组织培养液诱导的3种酶的总活性。本研究共鉴定出3个在侵染早期显著上调表达的植物细胞壁降解酶编码基因,为稻曲病病菌致病基因的克隆提供了关键候选基因。由结果还可以看出,稻曲病病菌可通过差异表达PCWDE基因及动态调节酶活性以适应水稻的组织特异性微环境。本研究结果可为深入解析稻曲病病菌特异性侵染水稻穗部的环境信号感知机制提供参考。
Abstract:
Rice false smut, caused by Ustilaginoidea virens, severely impacts rice yield and grain quality, posing a significant threat to food security. Plant cell wall-degrading enzymes (PCWDEs) are critical virulence factors for pathogens. To elucidate the role of PCWDEs in the infection of rice by U. virens, this study systematically identified 47 PCWDE-encoding genes based on the carbohydrate-active enzymes database (CAZy). Comparative genomic analysis revealed that the number of PCWDEs in U. virens was similar to that of biotrophic pathogens, but significantly lower than that of hemibiotrophic and saprophytic pathogens. Dynamic transcriptomic profiling showed that the relative expression levels of three key genes such as UV8b_01537, UV8b_03436, and UV8b_04918 were significantly upregulated at 48 hours post-inoculation on rice. Their encoded proteins involved in the degradation of cellulose, hemicellulose, and starch, respectively. Enzymatic activity assays using p-nitrophenol chromogenic substrates indicated that PS culture medium, rice leaf tissue, and panicle tissue culture solutions induced the production of α-glucosidase, β-glucosidase, and β-xylanase in two highly virulent strains. These enzymes degrade starch, cellulose, and hemicellulose, respectively. Total enzyme activities exhibited dynamic changes over time, peaking on the fourth day of culture under shaking conditions at 28 ℃ and 180 r/min, and showing a negative correlation with pathogenicity. Notably, panicle tissue culture solutions induced significantly lower total enzyme activities compared to leaf tissue solutions. This study identified three PCWDE-encoding genes that were significantly upregulated during the early stages of infection, providing critical candidates for functional gene cloning. The findings also demonstrate that U. virens adapts to host tissue-specific microenvironments by differentially expressing PCWDE genes and dynamically regulating enzyme activities. These results offer insights into the environmental signaling mechanisms underlying the pathogen’s specific infection of rice panicles and lay a foundation for further research.

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备注/Memo

备注/Memo:
收稿日期：2024-10-29基金项目：国家自然科学基金项目（32272512）；海南省种业实验室项目（B23YQ1514/B23CQ15EP）作者简介：高永煌（1999-），男，四川茂县人，硕士研究生，主要从事水稻稻曲病致病机制的研究。(E-mail)1059435886@qq.com通讯作者：刘永锋，(E-mail)liuyf@jaas.ac.cn
更新日期/Last Update: 2025-09-23