[1]姚林江,梁文化,陈涛,等.水稻分蘖数遗传位点挖掘与候选基因分析[J].江苏农业学报,2025,(08):1457-1465.[doi:doi:10.3969/j.issn.1000-4440.2025.08.001]
　YAO Linjiang,LIANG Wenhua,CHEN Tao,et al.Identification of genetic loci for rice tiller number and analysis of candidate genes[J].,2025,(08):1457-1465.[doi:doi:10.3969/j.issn.1000-4440.2025.08.001]
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水稻分蘖数遗传位点挖掘与候选基因分析()

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年08期
页码:
1457-1465
栏目:
遗传育种·生理生化
出版日期:
2025-08-31

文章信息/Info

Title:
Identification of genetic loci for rice tiller number and analysis of candidate genes
作者:
姚林江1; 梁文化2; 陈涛2; 朱镇2; 赵庆勇2; 李余生2; 姚姝2; 赵凌2; 周丽慧2; 赵春芳2
(1.江苏大学生命科学学院,江苏镇江212013；2.江苏省农业科学院粮食作物研究所/国家耐盐碱水稻技术创新中心华东中心,江苏南京210014)
Author(s):
YAO Linjiang1; LIANG Wenhua2; CHEN Tao2; ZHU Zhen2; ZHAO Qingyong2; LI Yusheng2; YAO Shu2; ZHAO Ling2; ZHOU Lihui2; ZHAO Chunfang2
(1.College of Life Science, Jiangsu University, Zhenjiang 212013, China;2.Institute of Food Crops, Jiangsu Academy of Agricultural Sciences/National Salt-tolerant Rice Technology Innovation Center, East China Center, Nanjing 210014, China)
关键词:
水稻; 分蘖数; Bin图谱; QTL定位
Keywords:
rice; tiller number; Bin map; QTL mapping
分类号:
S511
DOI:
doi:10.3969/j.issn.1000-4440.2025.08.001
文献标志码:
A
摘要:
分蘖数是水稻的一个重要农艺性状,直接影响水稻的产量。因此,挖掘水稻分蘖数相关基因,对分蘖数的遗传机制研究和品种改良具有重要意义。本研究利用分蘖数差异明显的粳稻TD70与籼稻 Kasalath构建的186份重组自交系（RIL）材料,结合2021年和2023年的分蘖数和基因型数据,利用数量性状基因座(QTL） IciMapping V3.4软件,采用完备区间作图法对控制水稻分蘖数的QTL进行鉴定。2年共检测到17个分蘖数相关的QTL,分别位于1号、2号、4号、5号、6号、7号、11号、12号染色体上,对数概率比值(LOD值)为2.66～9.14,可解释表型变异为1.48%～8.68%；QTL定位物理区间分析结果表明,本研究中有3个QTL与前人定位到的分蘖数QTL区间重合或者邻近,其中2个位点鉴定出分蘖数基因。qTN2-1和qTN4在2年试验中均被检测到,表现出较强的稳定性,其中qTN2-1位于2号染色体上7.12～7.25 Mb区间内,2年的LOD值分别为3.96和7.14,贡献率分别为7.58%和6.45%；qTN4位于4号染色体上2.43～2.67 Mb区间内,2年的LOD值分别为3.94和2.66,贡献率分别为6.86%和8.42%。对qTN2-1和qTN4定位区间进行亲本间序列的变异分析,发现6个基因编码区在亲本间存在错义突变。进一步根据单核苷酸多态性(SNP)的类型将RIL群体株系分成HapA和HapB 2种等位型,效应分析结果显示,不同等位型分蘖数差异显著或极显著；结合表达分析结果发现2个分蘖数相关的候选基因,为进一步解析水稻分蘖数的分子机制提供了理论依据,并为水稻高产育种提供了新位点。
Abstract:
Tiller number is a key agronomic trait that directly determines rice yield. Therefore, mining genes related to tiller number is crucial for the genetic mechanism investigation of tiller number and variety improvement. In this study, 186 recombinant inbred lines (RILs) were constructed from japonica rice TD70 and indica rice Kasalath, which had obvious differences in tiller number. Data of tiller-number and genotype collected in 2021 and 2023 were jointly analysed with the quantitative trait locus (QTL) IciMapping V3.4 software to identify rice tiller related QTLs by inclusive composite interval mapping method. A total of 17 QTLs related to tiller number were detected on Chromosome One, Chromosome Two, Chromosome Four, Chromosome Five, Chromosome Six, Chromosome Seven, Chromosome Eleven and Chromosome Twelve respectively in two years, with logarithm of odds (LOD) scores ranged from 2.66 to 9.14, and the phenotypic variation explained from 1.48% to 8.68%. QTL mapping physical interval analysis revealed that three of the QTLs overlapped or were adjacent to previously reported QTL loci related to tiller number, two of which were identified as tiller number genes. qTN2-1 and qTN4 were detected in two years and showed high stability. The qTN2-1 was mapped within 7.12-7.25 Mb on Chromosome Two, the LOD values were 3.96 and 7.14, and the contribution rates were 7.58% and 6.45% in 2021 and 2023, respectively. The qTN4 was mapped within 2.43-2.67 Mb on Chromosome Four, the LOD values were 3.94 and 2.66, and the contribution rates were 6.86% and 8.42% in 2021 and 2023, respectively. Variation analysis of sequences between parents within the mapping intervals of qTN2-1 and qTN4 showed that, there were missense mutations in the coding regions of six genes between parents. The RILs population strains were further classified into HapA and HapB alleles based on the type of single nucleotide polymorphism (SNP). Results of effect showed that, the tiller number showed significant or extremely significant difference between different alleles. Two candidate genes for tiller number were identified combined with results of expression analysis, which provided theoretical basis for analyzing the molecular mechanism of tiller number in rice and offered novel loci for high-yield rice breeding.

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备注/Memo

备注/Memo:
收稿日期：2024-11-06基金项目：现代农业产业技术体系建设专项(CARS-01-69)作者简介：姚林江(1997-),男,江苏连云港人,硕士研究生,主要从事水稻遗传育种研究。(Tel)18914725895；(E-mail)305527458@qq.com通讯作者：朱克明, (E-mail)uegzkg@sina.com.cn；张亚东,(E-mail)zhangyd@jaas.ac.cn
更新日期/Last Update: 2025-09-23