[1]梁巍骞,刘兰苑,陈锭娴,等.四种光唇鱼属鱼类线粒体DNA全序列测定及分析[J].江苏农业学报,2025,(06):1197-1206.[doi:doi:10.3969/j.issn.1000-4440.2025.06.016]
　LIANG Weiqian,LIU Lanyuan,CHEN Dingxian,et al.Complete sequence determination and analysis of mitogenome of four species in Acrossocheilus[J].,2025,(06):1197-1206.[doi:doi:10.3969/j.issn.1000-4440.2025.06.016]
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四种光唇鱼属鱼类线粒体DNA全序列测定及分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1197-1206
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-06-30

文章信息/Info

Title:
Complete sequence determination and analysis of mitogenome of four species in Acrossocheilus
作者:
梁巍骞1; 刘兰苑1; 陈锭娴1; 王凯丰1; 陈炜坚1; 林胜跃1; 叶树政1; 龚剑2; 桂林1; 李强1
(1.广州大学生命科学学院,广东广州510006；2.广州大学环境科学与工程学院,广东广州510006)
Author(s):
LIANG Weiqian1; LIU Lanyuan1; CHEN Dingxian1; WANG Kaifeng1; CHEN Weijian1; LIN Shengyue1; YE Shuzheng1; GONG Jian2; GUI Lin1; LI Qiang1
(1.School of Life Sciences, Guangzhou University, Guangzhou 510006, China;2.School of Environmental Science and Engineering, Guangzhou University, Guangzhou 510006, China)
关键词:
吉首光唇鱼; 侧条光唇鱼; 厚刺光唇鱼; 虹彩光唇鱼; 线粒体基因组; 系统发育分析
Keywords:
Acrossocheilus jishouensis; Acrossocheilus parallens; Acrossocheilus spinifer; Acrossocheilus iridescens; mitogenome; phylogenetic analysis
分类号:
S917.4
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.016
文献标志码:
A
摘要:
本研究采集并测量了吉首光唇鱼(Acrossocheilus jishouensis)、侧条光唇鱼(A. parallens)、厚刺光唇鱼(A. spinifer)和虹彩光唇鱼(A. iridescens)的线粒体DNA（mtDNA）序列。通过DNAstar Lasergene 7.0和MEGA7.0软件进行拼接获得4种光唇鱼属鱼类完整mtDNA序列,通过MITOSWebServer在线软件定位其编码蛋白质基因,采用tRNAscan-SE 2.0软件定位分析其转运RNA（tRNA）基因,利用BLAST同源性分析确定核糖体RNA（rRNA）基因和控制区。结合GenBank已发表的其他光唇鱼属鱼类和部分其他鲃亚科鱼类,构建系统发育树,分析4种光唇鱼属鱼类的系统进化关系。4种光唇鱼属鱼类mtDNA的核苷酸组成成分相似,序列长度为16 585～16 595 bp,均包括37个编码基因和1个非编码区(控制区)。8个tRNA基因(tRNAGln、tRNAAla、tRNAAsn、tRNACys、tRNATyr、tRNASer、tRNAGlu、tRNAPro)和ND6基因分布于轻链(L链),其余基因分布于重链(H链)。在13种编码蛋白质基因中,厚刺光唇鱼的ND2基因以ATC为起始密码子而区别于其他3种光唇鱼,侧条光唇鱼和厚刺光唇鱼的ND3基因起始密码子为GTG,其他2种光唇鱼则以ATG为起始密码子,其余基因起始密码子均为ATG或GTG,而终止密码子共有4种类型(TAA、TAG、TA和T)。22种tRNA基因,除了tRNASer编码的tRNA缺少DHU臂外,其余基因编码的tRNA均具有典型的三叶草结构。控制区变异较大,同时可以识别出对应的终止序列区、中央保守区和保守序列区。系统发育分析结果表明,光唇鱼属鱼类可以划分为2个支系,其中侧条光唇鱼与吉首光唇鱼亲缘关系最近,与虹彩光唇鱼亲缘关系最远。本研究系统分析了4种光唇鱼属鱼类线粒体DNA全序列,了解基因组结构、特点及其分类情况,为光唇鱼属鱼类的系统进化分析及其种质资源保护提供了理论依据。
Abstract:
In this study, samples were collected and mitochondrial DNA sequences were measured from Acrossocheilus jishouensis, A. parallens, A. spinifer and A. iridescens. The complete mtDNA sequences of four species were obtained through DNAstar Lasergene 7.0 and MEGA7.0 softwares. The protein-coding genes were located by MITOSWebServer online software, and the transfer RNA (tRNA) genes were identified and analyzed by tRNAscan-SE 2.0. The ribosomal RNA (rRNA) genes and control regions were identified by BLAST homology analysis. Incorporating other Acrossochelius species and selected representatives of the subfamily Barbinae available in GenBank, phylogenetic trees were constructed to analyze the phylogenetic relationships of the four Acrossochelius species. The nucleotide components of mtDNA of the four species were similar. The sequences were 16 585-16 595 bp in length, including 37 coding genes and one non-coding region (control region). Eight tRNA genes (tRNAGln, tRNAAla, tRNAAsn, tRNACys, tRNATyr, tRNASer, tRNAGlu, tRNAPro) and ND6 were located on the light strand (L strand), and the remaining genes were located on the heavy strand (H strand). Among the 13 protein-coding genes, the ND2 gene of A. spinifer used ATC as the start codon, which was different from the other three species. The start codons of ND3 genes of A. parallens and A. spinifer were GTG, while the other two species used ATG as the start codon, and the start codons of the remaining genes were ATG or GTG. There were four types of stop codons (TAA, TAG, TA and T). All 22 tRNA genes encoded tRNAs that possessed the typical cloverleaf secondary structure, except for the tRNA encoded by the tRNASer gene, which lacked the DHU arm. The variation of control region was obvious, but the corresponding termination sequence region, central conserved region and conserved sequence region were identified in control region. Phylogenetic analysis showed that the Acrossocheilus species could be divided into two clades. A. parallens was most closely related to A. jishouensis and most distantly related to A. iridescens. By systematically analyzing the complete mitochondrial DNA (mtDNA) sequences of four Acrossochelius species, this study elucidates their genome structure, characteristics, and taxonomic status. These findings provide a theoretical foundation for the phylogenetic analysis of the genus Acrossochelius and the conservation of its germplasm resources.

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备注/Memo

备注/Memo:
收稿日期：2024-10-25基金项目：国家自然科学基金项目(41673110)；广州市科技计划项目(201804010486)；广东省教育科学规划课题(2021GXJK097)作者简介：梁巍骞(2003-),男,广东佛山人,本科生,主要从事淡水鱼类生态与亲缘地理研究。(E-mail)liangweiqian1112@163.com。刘兰苑为共同第一作者。通讯作者：李强,(E-mai)LQ512328@163.com
更新日期/Last Update: 2025-07-16