[1]苏韦赫,吉星,张莉莉,等.江苏省鸡源沙门菌的分子流行病学特征分析[J].江苏农业学报,2025,(06):1188-1196.[doi:doi:10.3969/j.issn.1000-4440.2025.06.015]
　SU Weihe,JI Xing,ZHANG Lili,et al.Molecular epidemiological characteristics of Salmonella isolated from chickens in Jiangsu province[J].,2025,(06):1188-1196.[doi:doi:10.3969/j.issn.1000-4440.2025.06.015]
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江苏省鸡源沙门菌的分子流行病学特征分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1188-1196
栏目:
畜牧兽医·水产养殖·益虫饲养
出版日期:
2025-06-30

文章信息/Info

Title:
Molecular epidemiological characteristics of Salmonella isolated from chickens in Jiangsu province
作者:
苏韦赫1; 2; 吉星2; 张莉莉2; 王佳芸2; 何涛1; 2; 王冉1; 2
(1.江苏大学食品与生物工程学院,江苏镇江212013；2.江苏省农业科学院农产品质量安全与营养研究所/省部共建国家重点实验室培育基地——江苏省食品质量安全重点实验室,江苏南京210014)
Author(s):
SU Weihe1; 2; JI Xing2; ZHANG Lili2; WANG Jiayun2; HE Tao1; 2; WANG Ran1; 2
(1.School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China;2.Institute of Food Safety and Nutrition, Jiangsu Academy of Agricultural Sciences/Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base, Ministry of Science and Technology, Nanjing 210014, China)
关键词:
鸡源沙门菌; 流行病学; 血清型; 多位点序列分型; 耐药基因; 毒力基因
Keywords:
Salmonella isolated from chickens; epidemiology; serotypes; multilocus sequence typing; resistance genes; virulence genes
分类号:
S852.61
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.015
文献标志码:
A
摘要:
为明确江苏省鸡源沙门菌的分子流行病学特征,本研究通过采集江苏省8市规模化养鸡场病鸡粪便样本1 641份,分离沙门菌,并使用微量肉汤稀释法测定沙门菌分离菌株对抗菌药物的敏感性,基于全基因组测序技术分析分离菌株的耐药基因、毒力基因的携带情况,通过鉴定沙门菌的抗原结构式判断分离菌株的血清型,通过多位点序列分型（MLST）和系统发育树分析江苏省沙门菌的流行特征。结果表明,从1 641份鸡粪样品中共分离出266株沙门菌,分离率为16.21%；分离菌株对氨苄西林和磺胺甲口恶唑的耐药率较高,分别为56.39%和55.64%；多重耐药菌占比为53.38%。携带sul2、blaTEM-1和tetA等耐药基因的菌株比例较高,分别为59.40%、56.77%和39.10%。分离菌株的多位点序列分型(MLST)共鉴定出21种ST型,其中ST11型占比高达49.62%。血清型以肠炎、鸡白痢、鼠伤寒和印第安纳为主。分离得到的沙门菌共分为5个遗传进化分支,相同的养殖场分离株之间遗传进化关系比较接近,不同养殖场分离株的遗传关系较远。本研究结果为江苏省鸡源沙门菌的精准防治提供了依据。
Abstract:
In order to clarify the molecular epidemiological characteristics of Salmonella from chickens in Jiangsu province, 1 641 fecal samples of sick chickens from large-scale chicken farms in eight cities of Jiangsu province were collected to isolate Salmonella, and the sensitivity of Salmonella isolates to antibiotics was determined by microbroth dilution method. Based on the whole genome sequencing technology, the carrying status of drug resistance genes and virulence genes of the isolates was analyzed. The serotype of the isolates was determined by identifying the antigenic structure of Salmonella, and the epidemiological characteristics of Salmonella in Jiangsu province were analyzed by multilocus sequence typing (MLST) and phylogenetic tree. The results showed that 266 strains of Salmonella were isolated from 1 641 chicken manure samples, and the isolation rate was 16.21%. The resistance rates of the isolated strains to ampicillin and sulfamethoxazole were 56.39% and 55.64%, respectively. Multi-drug resistant bacteria accounted for 53.38%. The proportion of strains carrying sul2, blaTEM-1 and tetA genes was 59.40%, 56.77% and 39.10%, respectively. A total of 21 ST types were identified by MLST, of which ST11 accounted for 49.62%. The serotypes were mainly enteritis, pullorum disease, typhoid fever and Indiana. The Salmonella isolates were divided into five genetic evolution branches. The genetic evolution relationship between the same farm isolates was relatively close, and the genetic relationship between different farm isolates was far. The results of this study provide a basis for the precise prevention and control of Salmonella in chickens in Jiangsu province.

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备注/Memo

备注/Memo:
收稿日期：2024-06-19基金项目：“十四五”国家重点研发计划项目(2023YFD1800303)作者简介：苏韦赫(1998-),男,河南商丘人,硕士研究生,研究方向为细菌与噬菌体互作。(E-mail)swh19139013194@163.com通讯作者：何涛,(E-mail)vethetao@163.com；王冉,(E-mail) ranwang@jaas.ac.cn
更新日期/Last Update: 2025-07-16