[1]张承康,黄欣,李希荣,等.百香果果生炭疽菌小GTP酶基因Rab7的鉴定与功能分析[J].江苏农业学报,2025,(06):1107-1115.[doi:doi:10.3969/j.issn.1000-4440.2025.06.007]
　ZHANG Chengkang,HUANG Xin,LI Xirong,et al.Identification and functional analysis of the small GTPase gene Rab7 in Colletotrichum fructicola of Passiflora edulis[J].,2025,(06):1107-1115.[doi:doi:10.3969/j.issn.1000-4440.2025.06.007]
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百香果果生炭疽菌小GTP酶基因Rab7的鉴定与功能分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1107-1115
栏目:
植物保护
出版日期:
2025-06-30

文章信息/Info

Title:
Identification and functional analysis of the small GTPase gene Rab7 in Colletotrichum fructicola of Passiflora edulis
作者:
张承康1; 2; 黄欣1; 2; 李希荣1; 郭彦超1; 郭田龙1; 2; 陈美霞1
(1.宁德师范学院生物科学与工程学院省级产学研合作示范基地,福建宁德352100；2.福建农林大学生物农药与化学生物学教育部重点实验室,福建福州350002)
Author(s):
ZHANG Chengkang1; 2; HUANG Xin1; 2; LI Xirong1; GUO Yanchao1; GUO Tianlong1; 2; CHEN Meixia1; 2
(1.Provincial Demonstration Base for Industry-University-Research Cooperation, College of Biological Science and Engineering, Ningde Normal University, Ningde 352100, China；2.Key Laboratory of Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou 350002, China)
关键词:
百香果; 果生炭疽菌; 小GTP酶基因Rab7; 致病性
Keywords:
Passiflora edulis; Colletotrichum fructicola; small GTPase gene Rab7; pathogenicity
分类号:
S436.639
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.007
文献标志码:
A
摘要:
由果生炭疽菌(Colletotrichum fructicola)引起的百香果炭疽病是影响百香果(Passiflora edulis)产量和品质的重要病害,解析果生炭疽菌致病分子机制对病害防控具有重要意义。本研究鉴定得到百香果果生炭疽菌小GTP酶Rab7,并将其与多种真菌的Rab7蛋白氨基酸序列进行比对。系统发育分析结果表明,果生炭疽菌Rab7蛋白与酿酒酵母(Saccharomyces cerevisiae)Rab7蛋白的亲缘关系最远,而与胶孢炭疽菌(Colletotrichum gloeosporioides)、禾谷炭疽菌(Colletotrichum graminicola)Rab7蛋白的亲缘关系较近。利用同源重组技术构建Rab7基因敲除菌株△Rab7及Rab7基因回补菌株△Rab7/Rab7。表性分析发现,与野生型和△Rab7/Rab7相比,△Rab7在PDAY和MM培养基中的菌落直径显著下降,黑色素积累量显著增加,分生孢子产量显著减少。但△Rab7与野生型N425对H2O2胁迫的敏感性无显著差异。激光共聚焦显微镜观察发现Rab7-GFP融合蛋白定位于液泡膜,且△Rab7液泡呈现碎片化分布,表明Rab7基因通过调控果生炭疽菌液泡融合维持其正常液泡形态。本研究结果为解析果生炭疽菌的致病分子机制提供理论依据。
Abstract:
Anthracnose of Passiflora edulis caused by Colletotrichum fructicola is a significant disease affecting the yield and quality of passion fruit. Elucidating the pathogenic molecular mechanisms of Colletotrichum fructicola is of great significance for disease control. In this study, we identified the small GTPase Rab7 from C. fructicola and compared its amino acid sequences with those of Rab7 proteins from various fungi. Phylogenetic analysis revealed that the Rab7 protein of C. fructicola had the most distant relationship with the Rab7 protein of Saccharomyces cerevisiae, while it was closely related to the Rab7 proteins of Colletotrichum gloeosporioides and Colletotrichum graminicola. Using homologous recombination technology, we constructed the Rab7 gene knockout strain △Rab7 and the complemented strain △Rab7/Rab7. Phenotypic analysis showed that compared with the wild type and △Rab7/Rab7, the △Rab7 mutant exhibited significantly reduced colony diameter on PDAY and MM media, significantly increased melanin accumulation, and significantly decreased conidial production. However, △Rab7 and the wild type N425 did not show significant differences in sensitivity to H2O2 stress. Confocal laser scanning microscopy observations indicated that the Rab7-GFP fusion protein was localized to the vacuolar membrane, and the vacuoles in △Rab7 appeared fragmented, suggesting that Rab7 gene maintained normal vacuolar morphology in C. fructicola by regulating vacuolar fusion. This study provides a theoretical basis for elucidating the molecular mechanisms of pathogenicity in C. fructicola.

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备注/Memo

备注/Memo:
收稿日期：2024-11-06基金项目：福建省自然科学基金项目(2021J05254)；宁德师范学院创新团队项目(2022T01)作者简介：张承康(1988-),男,福建宁德人,博士,副教授,主要从事植物病原菌致病分子机理的研究。(E-mail)shin_ichi@126.com通讯作者：陈美霞,(E-mail)cmx_101019@163.com
更新日期/Last Update: 2025-07-16