[1]朱志炎,张德清,周厚英,等.半夏ARF转录因子家族的全基因组鉴定及生物信息学分析[J].江苏农业学报,2025,(06):1095-1106.[doi:doi:10.3969/j.issn.1000-4440.2025.06.006]
　ZHU Zhiyan,ZHANG Deqing,ZHOU Houying,et al.Genome-wide identification and bioinformatics analysis of the ARF transcription factor family in Pinellia ternata[J].,2025,(06):1095-1106.[doi:doi:10.3969/j.issn.1000-4440.2025.06.006]
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半夏ARF转录因子家族的全基因组鉴定及生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年06期
页码:
1095-1106
栏目:
遗传育种·生理生化
出版日期:
2025-06-30

文章信息/Info

Title:
Genome-wide identification and bioinformatics analysis of the ARF transcription factor family in Pinellia ternata
作者:
朱志炎1; 张德清1; 周厚英2; 何勇2; 田志宏2
(1.南通师范高等专科学校初等教育学院,江苏南通226010；2.长江大学生命科学学院,湖北荆州434025)
Author(s):
ZHU Zhiyan1; ZHANG Deqing1; ZHOU Houying2; HE Yong2; TIAN Zhihong2
(1.College of Elementary Education, Nantong Normal College, Nantong 226010, China;2.College of Life Science, Yangtze University, Jingzhou 434025, China)
关键词:
半夏; ARF转录因子家族; 倒苗; 生物信息学
Keywords:
Pinellia ternate; ARF transcription factor family; sprout tumble; bioinformatics
分类号:
S567.2
DOI:
doi:10.3969/j.issn.1000-4440.2025.06.006
文献标志码:
A
摘要:
半夏(Pinellia ternate)是一种重要的药用植物,其在生长过程中对光照和温度极为敏感,易发生倒苗现象,导致产量降低。本研究基于国家生物信息中心发布的半夏基因组数据,鉴定出25个PtARF家族成员,并通过系统进化分析将其划分为4个亚族。蛋白质理化性质分析结果表明,半夏PtARF均为亲水性不稳定蛋白,大部分PtARF定位于细胞核中。蛋白质三级结构预测结果显示,亲缘关系较近的PtARF家族成员的三级结构高度相似,表明部分PtARF可能存在功能冗余。系统进化分析和共线性分析结果表明,半夏和水稻的ARF基因亲缘关系较近。半夏ARF家族基因启动子区域中,数量最多的顺式作用元件为生物及非生物胁迫响应元件,其次是植物激素响应元件,植物生长与发育响应元件数量最少。这一结果表明,PtARF可能广泛参与半夏对逆境胁迫的响应。进一步的研究结果表明,PtARF能够响应弱光胁迫和高温胁迫,可能参与半夏倒苗和块茎发育的调控。本研究为半夏抗逆育种及栽培技术优化提供了重要的理论依据。
Abstract:
Pinellia ternata is an important medicinal plant. During its growth, it is extremely sensitive to light and temperature, and prone to the phenomenon of seedling collapse, which leads to a reduction in yield. In this study, based on the genomic data of Pinellia ternata released by the China National Center for Bioinformation, 25 members of the PtARF family were identified and divided into four sub-families through phylogenetic analysis. The results of protein physicochemical property analysis showed that all PtARFs in Pinellia ternata were hydrophilic and unstable proteins, and most PtARFs were located in the cell nucleus. The prediction of the protein tertiary structure indicated that the tertiary structures of PtARF family members with close genetic relationships were highly similar, suggesting potential functional redundancy among some PtARF proteins. The results of phylogenetic analysis and collinearity analysis showed that the ARF genes of Pinellia ternata and rice were closely related. In the promoter regions of the ARF family genes in Pinellia ternata, the cis-acting elements with the largest number were those related to biotic and abiotic stress responses, followed by plant hormone-responsive elements, and the elements related to plant growth and development responses were the least in number. This result indicated that PtARFs may be widely involved in the response of Pinellia ternata to stress. Further research results showed that PtARF could respond to low-light stress and high-temperature stress, and may be involved in the regulation of seedling collapse and tuber development in Pinellia ternata. This study provides an important theoretical basis for stress-resistant breeding and optimization of cultivation techniques of Pinellia ternata.

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备注/Memo

备注/Memo:
收稿日期：2024-09-24基金项目：江苏省高等学校基础科学(自然科学)研究面上项目(24KJD210003)；南通师范高等专科学校青年项目(TSGZ2022Q02)；湿地生态与农业利用教育部工程研究中心开放基金项目(KF202321)作者简介：朱志炎(1989-),男,江苏南通人,博士,讲师,主要从事植物与微生物互作研究。(E-mail)201673036@yangtzeu.edu.cn
更新日期/Last Update: 2025-07-16