[1]许乐乐,黄倩敏,李永芹,等.健康广藿香与患病广藿香根际土壤微生物群落的结构[J].江苏农业学报,2025,(04):702-714.[doi:doi:10.3969/j.issn.1000-4440.2025.04.009]
　XU Lele,HUANG Qianmin,LI Yongqin,et al.Microbial community structure in rhizosphere soil of healthy and diseased patchouli (Pogostemon cablin)[J].,2025,(04):702-714.[doi:doi:10.3969/j.issn.1000-4440.2025.04.009]
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健康广藿香与患病广藿香根际土壤微生物群落的结构()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年04期
页码:
702-714
栏目:
耕作栽培·资源环境
出版日期:
2025-04-30

文章信息/Info

Title:
Microbial community structure in rhizosphere soil of healthy and diseased patchouli (Pogostemon cablin)
作者:
许乐乐; 黄倩敏; 李永芹; 王锂韫
(岭南师范学院生命科学与技术学院,广东湛江524048)
Author(s):
XU Lele; HUANG Qianmin; LI Yongqin; WANG Liyun
(School of Life Science and Technology, Lingnan Normal University, Zhanjiang 524048, China)
关键词:
广藿香; 根际土壤; 微生物群落; 高通量测序; 病害防治
Keywords:
Pogostemon cablin; rhizosphere soil; microbial community; high-throughput sequencing; disease prevention and control
分类号:
S154.3
DOI:
doi:10.3969/j.issn.1000-4440.2025.04.009
文献标志码:
A
摘要:
为探究健康广藿香与患病广藿香根际土壤中微生物群落的结构组成和多样性,本研究利用高通量测序技术分别对健康广藿香和患病广藿香根际土壤微生物群落进行测序及功能预测分析。结果显示,广藿香患病后,根际土壤中全磷含量显著升高(P<0.05)。从健康广藿香和患病广藿香根际土壤中共得到801个真菌操作分类单元（OTU）和3 477个细菌OTU,患病广藿香根际土壤真菌数量明显增加,细菌数量则有所下降。健康广藿香和患病广藿香根际土壤中微生物群落门和属的组成没有变化,但部分门和属的相对丰度却存在差异。患病后,在门水平上,酸杆菌门(Acidobacteria)和疣微菌门(Verrucomicrobia)细菌、子囊菌门(Ascomycota)真菌相对丰度显著下降,厚壁菌门(Firmicutes)细菌、担子菌门(Basidiomycota)真菌相对丰度则显著升高；在属水平上,节杆菌属(Paenarthrobacter)、罗尔斯通菌属(Ralstonia)和异样根瘤菌属(Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium)细菌及裸节菌属(Talaromyces)真菌的相对丰度显著增加,柄孢壳属(Podospora)、Conlarium属和节丛孢属(Arthrobotrys)真菌的相对丰度显著下降。相关性分析结果表明,健康广藿香与患病广藿香根际微生物门类与土壤环境因子的相关性表现出明显差异,患病广藿香中土壤理化指标与更多细菌门类的相对丰度具有相关性,而健康植株则表现为土壤理化指标与更多真菌门类的相对丰度具有相关性。FAPROTAX功能预测结果表明,植物病原体、尿素分解、芳烃降解和碳氢化合物降解等4类细菌功能相对丰度在健康广藿香与患病广藿香根际土壤间具有明显差异；FUNGuild功能预测结果显示,健康广藿香和患病广藿香根际土壤真菌营养类型病理-腐生-共生营养型和共生营养型相对丰度差异显著,患病植株植物病原菌功能相对丰度显著提升。功能预测结果表明，广藿香病害的发生与病原微生物相对丰度的改变存在一定联系。本研究结果可为广藿香病害的防治提供一定的理论依据。
Abstract:
To explore the structure and diversity of the rhizosphere soil microbial community of healthy and diseased Pogostemon cablin (patchouli), high-throughput sequencing technology was employed to sequence and conduct functional prediction analyses on the microbial communities in the rhizosphere soil of both healthy and diseased patchouli plants. The results indicated that after patchouli plants were diseased, the total phosphorus content in the rhizosphere soil increased significantly (P<0.05). A total of 801 fungal operational taxonomic units (OTUs) and 3 477 bacterial OTUs were identified in the rhizosphere soil of both healthy and diseased patchouli plants. The number of fungi in the rhizosphere soil of diseased patchouli plants increased significantly, while the number of bacteria decreased. The composition of microbial phyla and genera in the rhizosphere soil of healthy and diseased patchouli plants remained unchanged, but differences were observed in the relative abundance of some phyla and genera. After the plants were diseased, at the phylum level, the relative abundances of Acidobacteria and Verrucomicrobia bacteria, as well as Ascomycota fungi decreased significantly, while the relative abundances of Firmicutes bacteria and Basidiomycota fungi increased significantly. At the genus level, the relative abundances of Paenarthrobacter, Ralstonia, and Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium bacteria, as well as Talaromyces fungi increased significantly, while the relative abundances of Podospora, Conlarium and Arthrobotrys fungi decreased significantly. Correlation analysis results showed that there was an obvious difference in the correlation between the rhizosphere microbial phyla of healthy and diseased patchouli plants and soil environmental factors. In diseased patchouli plants, soil physicochemical indicators were correlated with the relative abundance of more bacterial phyla, while in healthy plants, soil physicochemical indicators were correlated with the relative abundance of more fungal phyla. FAPROTAX functional prediction results indicated that there were obvious differences in the relative abundance of four types of bacterial functions, including plant pathogen, ureolysis, aromatic hydrocarbon degradation and hydrocarbon degradation, between the rhizosphere soil of healthy and diseased patchouli plants. FUNGuild functional prediction results showed that there were significant differences in the relative abundances of pathological-saprophytic-symbiotic and symbiotic nutritional types of fungal nutritional types in the rhizosphere soil of healthy and diseased patchouli plants. The relative abundance of plant pathogens in diseased plants increased significantly. The functional prediction results suggested that there was a certain correlation between the occurrence of patchouli disease and changes in the relative abundance of pathogenic microorganisms. The findings of this study can provide a theoretical basis for the prevention and control of patchouli diseases.

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备注/Memo

备注/Memo:
收稿日期：2024-07-30基金项目：湛江市科技计划项目(2022A01054)；广东省教育厅项目(2021KCXTD039)作者简介：许乐乐(1981-),男,广东湛江人,博士,助理研究员,研究方向为生物计算与计算机仿生学。(Tel)18934029616；(E-mail)xulees@163.com通讯作者：李永芹,(E-mail)lyongqin@163.com；王锂韫,(E-mail)wly@lingnan.edu.cn
更新日期/Last Update: 2025-05-26