[1]王籽懿,韩伟,金鸿飚,等.高寒黑土区不同轮作模式对马铃薯根际土壤细菌群落结构与功能的影响[J].江苏农业学报,2025,(02):286-295.[doi:doi:10.3969/j.issn.1000-4440.2025.02.009]
　WANG Ziyi,HAN Wei,JIN Hongbiao,et al.Effects of different crop rotation modes on the structure and function of bacterial communities in potato rhizosphere soil in high-altitude black soil areas[J].,2025,(02):286-295.[doi:doi:10.3969/j.issn.1000-4440.2025.02.009]
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高寒黑土区不同轮作模式对马铃薯根际土壤细菌群落结构与功能的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2025年02期
页码:
286-295
栏目:
耕作栽培·资源环境
出版日期:
2025-02-28

文章信息/Info

Title:
Effects of different crop rotation modes on the structure and function of bacterial communities in potato rhizosphere soil in high-altitude black soil areas
作者:
王籽懿1; 韩伟2; 金鸿飚3; 黄修梅1; 李明1
(1.内蒙古农业大学,内蒙古呼和浩特010019；2.雪川农业集团股份有限公司,河北张家口075000；3.呼伦贝尔市农牧科学研究所,内蒙古呼伦贝尔021000)
Author(s):
WANG Ziyi1; HAN Wei2; JIN Hongbiao3; HUANG Xiumei1; LI Ming1
(1.Inner Mongolia Agricultural University, Hohhot 010019, China;2.Xuechuan Agricultural Group Co., Ltd., Zhangjiakou 075000, China;3.Hulunbuir Institute of Agriculture and Animal Husbandry, Hulunbuir 021000, China)
关键词:
高寒黑土区; 轮作模式; 马铃薯; 根际土壤; 细菌群落结构; 细菌群落功能
Keywords:
high-altitude black soil areas; rotation pattern; potato; rhizosphere soil; bacterial community structure; bacterial community function
分类号:
S154.3;S532
DOI:
doi:10.3969/j.issn.1000-4440.2025.02.009
文献标志码:
A
摘要:
为明确不同轮作模式对马铃薯根际土壤细菌群落的影响,本研究以马铃薯-小麦-甜菜-马铃薯(A处理)、马铃薯-水飞蓟-小麦-马铃薯(B处理)、小麦-甜菜-小麦-马铃薯(C处理),油菜-小麦-甜菜-马铃薯(D处理),小麦-油菜-大麦-马铃薯(E处理)和油菜-小麦-马铃薯-马铃薯(CK，对照)6种种植模式为研究对象,采用细菌16S区的高通量测序技术,对比分析当地6种种植模式对马铃薯根际土壤细菌群落结构和功能的影响。结果表明,6种轮作模式的土壤样本共获得36 420个扩增子序列变异(ASV),划分为45门、119纲、298目、451科、790属和357种。不同轮作模式马铃薯根际土壤主要优势菌群同为变形菌门、酸杆菌门、拟杆菌门、芽单胞菌门、放线菌门、浮霉菌门、疣微菌门、绿弯菌门、黏球菌门、髌骨菌门,但主要优势菌群的相对丰度在不同轮作模式中存在差异。与CK相比,轮作明显提高了细菌中酸杆菌门和芽单胞菌门细菌的相对丰度,而放线菌门细菌相对丰度明显降低,溶杆菌属细菌以及与碳、氮相关的功能菌相对丰度升高。组间群落差异分析(LEfSe)结果表明,在细菌群落中共鉴定出38个差异物种。通过基因功能预测发现,一级功能中新陈代谢的相对丰度最大(62.04%～62.46%),相对丰度大于1.0%的二级功能代谢通路有19类,其中碳水化合物代谢的相对丰度最高(12.93%～13.29%)。综上所述,轮作改变了土壤细菌群落结构,提高了土壤细菌中的优势功能菌,其中以小麦-甜菜-小麦-马铃薯轮作模式效果最好,更适宜高寒黑土区马铃薯产业的可持续发展。
Abstract:
To clarify the effects of different rotation patterns on bacterial communities in potato rhizosphere soil, six planting patterns of potato-wheat-sugar beet-potato (A treatment), potato-milk thistle-wheat-potato (B treatment), wheat-sugar beet-wheat-potato (C treatment), oilseed rape-wheat-sugar beet-potato (D treatment), wheat-oilseed rape-barley-potato (E treatment) and oilseed rape-wheat-potato-potato (CK, control) were studied. The high-throughput sequencing technology of bacterial 16S region was used to compare and analyze the effects of six local planting patterns on the structure and function of bacterial communities in potato rhizosphere soil. The results showed that a total of 36 420 amplicon sequence variations (ASVs) were obtained from the soil samples under the six rotation patterns, which were divided into 45 phyla, 119 classes, 298 orders, 451 families, 790 genera and 357 species. The dominant bacteria in potato rhizosphere soil under different rotation modes were Proteobacteria, Acidobacteria, Bacteroidetes, Gemmatimonadota, Actinobacteria, Planctomycetota, Verrucomicrobiota, Chloroflexi, Myxococcota and Patescibacteria. However, the relative abundance of the dominant bacteria was different under different rotation patterns. Compared with CK, rotation significantly increased the relative abundance of Acidobacteria and Gemmatimonadota, while the relative abundance of Actinobacteria decreased significantly. In addition, the relative abundance of Lysobacter and functional bacteria related to carbon and nitrogen increased. The results of inter-group community difference analysis (LEfSe) showed that 38 differential species in the bacterial community were identified. Through gene function prediction, it was found that the relative abundance of metabolism in primary function was the highest (62.04%-62.46%). There were 19 categories of secondary functional metabolic pathways with relative abundance greater than 1.0%, among which carbohydrate metabolism had the highest relative abundance (12.93%-13.29%). In summary, rotation changed the soil bacterial community structure and increased the dominant functional bacteria in soil bacteria. In this study, the rotation mode of wheat-sugar beet-wheat-potato had the best effect, which was more suitable for the sustainable development of potato industry in high-altitude black soil areas.

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备注/Memo

备注/Memo:
收稿日期：2024-04-22基金项目：内蒙古自治区重点研发和成果转化计划项目(2022YFDZ0010)；河北省科技计划结转项目(21326320D)；内蒙古自治区农牧厅基层农业推广体系改革与建设补充及重大农业技术协同推广资金项目作者简介：王籽懿(1996-),女,河北张家口人,硕士研究生,主要研究方向为作物栽培。(E-mail)1754899519@qq.com通讯作者：黄修梅,(E-mail)huangxm0404@126.com
更新日期/Last Update: 2025-03-27