[1]孙国胜,张昌伟,山溪,等.基于KASP标记的厚皮甜瓜种质资源遗传多样性分析及指纹图谱构建[J].江苏农业学报,2024,(12):2349-2355.[doi:doi:10.3969/j.issn.1000-4440.2024.12.018]
　SUN Guosheng,ZHANG Changwei,SHAN Xi,et al.Analysis of genetic diversity and construction of fingerprints for core germplasms of muskmelon based on KASP markers[J].,2024,(12):2349-2355.[doi:doi:10.3969/j.issn.1000-4440.2024.12.018]
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基于KASP标记的厚皮甜瓜种质资源遗传多样性分析及指纹图谱构建()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2349-2355
栏目:
园艺
出版日期:
2024-12-30

文章信息/Info

Title:
Analysis of genetic diversity and construction of fingerprints for core germplasms of muskmelon based on KASP markers
作者:
孙国胜1; 2; 张昌伟2; 山溪1; 许丁帆1; 戴忠良1; 张振超1; 马志虎1
(1.江苏丘陵地区镇江农业科学研究所,江苏镇江212400;2.南京农业大学,江苏南京210095)
Author(s):
SUN Guosheng1; 2; ZHANG Changwei2; SHAN Xi1; XU Dingfan1; DAI Zhongliang1; ZHANG Zhenchao1; MA Zhihu1
(1.Zhenjiang Institute of Agricultural Sciences in Hilly Area of Jiangsu Province, Zhenjiang 212400, China;2.Nanjing Agricultural University, Nanjing 210095, China)
关键词:
厚皮甜瓜; 单核苷酸多态性; 竞争性等位基因特异性PCR; 遗传多样性; 指纹图谱
Keywords:
muskmelon; single nucleotide polymorphism (SNP); competitive allele-specific PCR (KASP); genetic diversity; fingerprint
分类号:
S652.9
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.018
文献标志码:
A
摘要:
本研究通过竞争性等位基因特异性PCR（KASP）标记对厚皮甜瓜核心种质资源进行多态性分析,以期筛选厚皮甜瓜种质资源的单核苷酸多态性（SNP）标记,应用于厚皮甜瓜种质资源及品种的纯度鉴定和指纹图谱构建。从公布的297份甜瓜重测序数据中挑选95份厚皮甜瓜重测序数据进行SNP标记开发,对课题组的20份核心厚皮甜瓜种质资源进行多态性分析。结果显示,共计开发50个SNP标记,分布在甜瓜12条染色体中。50个SNP标记中,有23个SNP标记能够将本研究中的19份核心厚皮甜瓜种质资源进行分型,其中Marker33、Marker38、Marker23、Marker36、Marker47、Marker42 6个标记能够完全区分19份甜瓜种质资源，本研究结果可以为后期厚皮甜瓜种质资源和品种纯度鉴定提供有利的支撑。
Abstract:
In this study, we analyzed the polymorphism of core resources of muskmelon by competitive allele-specific PCR (KASP) markers, with a view to screening single nucleotide polymorphism (SNP) markers of muskmelon resources, which could be applied to the purity identification and fingerprint construction of the parents and hybrids of muskmelon. From the published 297 melon resequencing data, 95 muskmelon resequencing data were selected for SNP marker development, and polymorphism analysis was performed on 20 core muskmelon germplasm resources of the subject group. The results showed that a total of 50 SNP markers were developed, and distributed on 12 chromosomes of muskmelon. Among the 50 SNP markers, 23 SNP markers could be used to genotype 19 core muskmelon resources. Six markers including Marker33, Marker38, Marker23, Marker36, Marker47 and Marker42 could completely distinguish 19 muskmelon germplasm resources. The results of this study provide favorable support for the identification of muskmelon resources and variety purity in the later stage.

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备注/Memo

备注/Memo:
收稿日期：2024-03-05基金项目：江苏省种业振兴揭榜挂帅项目[JBGS(2021)075]；江苏现代农业(西甜瓜)产业技术体系甜瓜育种栽培创新团队项目[JATS(2023)137]作者简介：孙国胜(1987-),男,山东莱州人,硕士,助理研究员,研究方向为蔬菜作物遗传育种。(E-mail)sgs8725@163.com通讯作者：马志虎,(E-mail)mazhihu830@163.com
更新日期/Last Update: 2025-01-23