[1]梁靖,王调叶,刘雅诗,等.4种葡萄SOD家族基因鉴定与表达分析[J].江苏农业学报,2024,(12):2334-2348.[doi:doi:10.3969/j.issn.1000-4440.2024.12.017]
 LIANG Jing,WANG Tiaoye,LIU Yashi,et al.Identification and expression analysis of SOD family genes in four kinds of grapes[J].,2024,(12):2334-2348.[doi:doi:10.3969/j.issn.1000-4440.2024.12.017]
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4种葡萄SOD家族基因鉴定与表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2334-2348
栏目:
园艺
出版日期:
2024-12-30

文章信息/Info

Title:
Identification and expression analysis of SOD family genes in four kinds of grapes
作者:
梁靖王调叶刘雅诗孙莹莹陈佰鸿褚明宇
(甘肃农业大学园艺学院,甘肃兰州730070)
Author(s):
LIANG JingWANG TiaoyeLIU YashiSUN YingyingCHEN BaihongCHU Mingyu
(College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China)
关键词:
葡萄SOD基因基因家族生物信息学表达分析
Keywords:
grapeSOD genegene familybioinformaticsexpression analysis
分类号:
S663.1
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.017
文献标志码:
A
摘要:
超氧化物歧化酶(SOD)在植物体氧化与抗氧化平衡中起至关重要的作用。本研究对4种葡萄SOD家族基因进行了全基因组鉴定和表达分析。鉴定结果显示,4种葡萄共有45个SOD基因,其中黑比诺葡萄12个、山葡萄8个、河岸葡萄15个、圆叶葡萄10个,SOD基因随机分布在染色体上;葡萄SOD基因启动子中有多个激素、生长发育、逆境胁迫等响应顺式作用元件;种内共线性分析结果显示,黑比诺葡萄、山葡萄和河岸葡萄SOD基因中仅含有1对共线性关系,而圆叶葡萄SOD基因中含有6对共线性关系;qRT-PCR结果表明VaSOD基因对氯化钠(NaCl)、水杨酸(SA)、脱落酸(ABA)、低温(4 ℃)等不同非生物胁迫的响应明显。4种葡萄SOD基因之间碱基序列相对保守,亲缘关系较近。本研究结果为进一步解析葡萄SOD 家族基因在其生长发育和非生物胁迫应答中的作用机理奠定了基础。
Abstract:
Superoxide dismutase (SOD) plays an important role in the balance between oxidation and antioxidation in plants. In this study, the whole genome identification and expression analysis of SOD family genes in four kinds of grapes were carried out. The identification results showed that there were 45 SOD genes in the four kinds of grapes, including 12 genes in Pinot Noir, eight genes in Vitis amurensis, 15 genes in Vitis riparia and 10 genes in Vitis rotundifolia. The SOD genes were randomly distributed on chromosomes. There were many cis-acting elements in the promoter of grape SOD gene, such as hormones, growth and development, stress response and so on. The results of intraspecific collinearity analysis showed that there was only one pair of collinearity in the SOD genes of Pinot Noir, Vitis amurensis and Vitis riparia, while there were six pairs of collinearity in the SOD genes of Vitis rotundifolia. The results of qRT-PCR showed that the VaSOD gene responded significantly to different abiotic stresses such as sodium chloride (NaCl), salicylic acid (SA), abscisic acid (ABA) and low temperature (4 ℃). The base sequence of SOD genes in four kinds of grapes was relatively conservative and the genetic relationship was relatively close. The results of this study can lay a foundation for further analysis of the mechanism of grape SOD family genes in their growth and development and abiotic stress response.

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备注/Memo

备注/Memo:
收稿日期:2024-01-12基金项目:国家自然科学基金项目(32060671);2021甘肃省青年人才托举工程项目(GXH20210611-01);甘肃农业大学大学生创新创业训练计划项目(S202110733035)作者简介:梁靖(2000-),女,甘肃武威人,硕士研究生,主要从事果树生理与生物技术方面的研究。(E-mail)1436387524@qq.com通讯作者:褚明宇,(E-mail)chu.my@foxmail.com
更新日期/Last Update: 2025-01-23