[1]胡子贤,许慧,邓鹏飞,等.结香患根腐病植株根际土壤微生物群落组成与多样性[J].江苏农业学报,2024,(12):2254-2265.[doi:doi:10.3969/j.issn.1000-4440.2024.12.008]
　HU Zixian,XU Hui,DENG Pengfei,et al.Composition and diversity of microbial community in rhizosphere soil of Edgeworthia chrysantha Lindl. with root rot disease[J].,2024,(12):2254-2265.[doi:doi:10.3969/j.issn.1000-4440.2024.12.008]
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结香患根腐病植株根际土壤微生物群落组成与多样性()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年12期
页码:
2254-2265
栏目:
植物保护
出版日期:
2024-12-30

文章信息/Info

Title:
Composition and diversity of microbial community in rhizosphere soil of Edgeworthia chrysantha Lindl. with root rot disease
作者:
胡子贤; 许慧; 邓鹏飞; 王佳琪; 杜子滔; 徐小牛
(安徽农业大学林学与园林学院,安徽合肥230036)
Author(s):
HU Zixian; XU Hui; DENG Pengfei; WANG Jiaqi; DU Zitao; XU Xiaoniu
(School of Forestry & Landscape Architecture, Anhui Agricultural University, Hefei 230036, China)
关键词:
结香; 根腐病; 根际土壤; 高通量测序; 细菌; 真菌; 多样性
Keywords:
Edgeworthia chrysantha Lindl.; root rot; rhizosphere soil; high-throughput sequencing; bacteria; fungi; diversity
分类号:
S435.671
DOI:
doi:10.3969/j.issn.1000-4440.2024.12.008
文献标志码:
A
摘要:
根腐病是影响结香产业可持续发展的重要病害之一,为了探明结香根腐病的发病机制,筛选潜在病原菌,研发有效防治药剂，本研究以安徽省潜山市根腐病高发的结香林作为研究对象,采用高通量测序技术(Illumina MiSeq),分析和比较了结香健康植株和患病植株根际土壤理化性质、细菌和真菌的群落结构及多样性。结果表明,患病植株根际土壤细菌和真菌丰富度指数(Chao1指数)分别较健康植株显著降低13.74%和45.83%,细菌多样性(Shannon指数)没有显著变化,但真菌多样性(Shannon指数)显著高于健康植株。与健康植株根际土壤相比,患病植株根际土壤黏细菌属(Byssovorax)相对丰度显著增加(P＜0.05)，真菌葡萄座腔菌属(Botryosphaeria)和蜡壳耳目(Sebacinales)未定属相对丰度极显著增加(P＜0.01)。患病植株根际土壤有机碳含量显著高于健康植株根际土壤。综上,结香在感染根腐病后,根际土壤细菌和真菌丰富度下降,真菌群落多样性升高,初步确定黏细菌属菌、葡萄座腔菌属菌和蜡壳耳目未定属菌可能是引起结香患根腐病的病原菌,本研究结果为后续开展结香致病菌的分离和培养提供了候选菌群。
Abstract:
Root rot is one of the important diseases affecting the sustainable development of Edgeworthia chrysantha Lindl. industry. To explore the pathogenesis of root rot of Edgeworthia chrysantha Lindl., screen potential pathogens, and develop effective control agents, this study took the Edgeworthia chrysantha Lindl. forest with high incidence of root rot in Qianshan City, Anhui province as the research object, and used high-throughput sequencing technology (IlluminaMiSeq) to analyze and compare the rhizosphere soil physical and chemical properties, bacterial and fungal community structure and diversity of healthy plants and diseased plants. The results showed that compared with healthy plants, the bacterial richness index (Chao1 index) and fungal richness index (Chao1 index) in rhizosphere soil of diseased plants were significantly reduced by 13.74% and 45.83%, respectively, and the bacterial diversity (Shannon index) did not change significantly, but the fungal diversity (Shannon index) was significantly increased. Compared with the rhizosphere soil of healthy plants, the relative abundance of Byssovorax in the rhizosphere soil of diseased plants increased significantly (P<0.05), and the relative abundance of Botryosphaeria and Sebacinales＿unclassified increased significantly (P<0.01). The organic carbon content in rhizosphere soil of diseased plants was significantly higher than that of healthy plants. In summary, after the infection of root rot, the richness of bacteria and fungi in rhizosphere soil decreased, and the diversity of fungal community increased. It was preliminarily determined that Byssovorax, Botryosphaeria and Sebacinales＿unclassified may be the pathogens causing root rot. The results of this study provide a candidate flora for the subsequent isolation and culture of pathogenic bacteria.

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备注/Memo

备注/Memo:
收稿日期：2023-12-28基金项目：安庆市科技攻关项目(KJ2021119);潜山市林业科技创新项目(KJ20200439)作者简介：胡子贤(1993-),男,安徽马鞍山人,硕士研究生,研究方向为森林培育。(E-mail)344106912@qq.com通讯作者：徐小牛,(E-mail)xnxu2007@ahau.edu.cn
更新日期/Last Update: 2025-01-23