[1]李玮,沈琦,古再丽阿依·司马义,等.不同生育期杏树果实中邻苯二甲酸酯含量及其在亚细胞组分中分布的变化与二者的关联性分析[J].江苏农业学报,2024,(11):2149-2162.[doi:doi:10.3969/j.issn.1000-4440.2024.11.019]
　LI Wei,SHEN Qi,Guzailiayi·Simayi,et al.Changes of phthalic acid ester content, subcellular distribution in apricot fruits at different growth stages and their correlation analysis[J].,2024,(11):2149-2162.[doi:doi:10.3969/j.issn.1000-4440.2024.11.019]
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不同生育期杏树果实中邻苯二甲酸酯含量及其在亚细胞组分中分布的变化与二者的关联性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
2149-2162
栏目:
园艺
出版日期:
2024-11-30

文章信息/Info

Title:
Changes of phthalic acid ester content, subcellular distribution in apricot fruits at different growth stages and their correlation analysis
作者:
李玮; 沈琦; 古再丽阿依·司马义; 张路思; 贺云云; 王伟
(新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052)
Author(s):
LI Wei; SHEN Qi; Guzailiayi·Simayi; ZHANG Lusi; HE Yunyun; WANG Wei
(College of Food Science and Pharmacy, Xinjiang Agricultural University, Urumqi 830052, China)
关键词:
邻苯二甲酸酯; 生育期; 杏果; 亚细胞分布; 食品安全; 关联性分析
Keywords:
phthalates; growth period; apricot fruit; subcellular distribution; food safety; correlation analysis
分类号:
TS207.3
DOI:
doi:10.3969/j.issn.1000-4440.2024.11.019
文献标志码:
A
摘要:
为了明确不同生育期杏树果实中邻苯二甲酸酯(PAEs)含量及其在亚细胞组分中分布的变化与二者的关联性,在新疆6个杏果主产区设置试验点,采集杏树不同生育期的杏果样品,综合气相色谱串联质谱、差速离心法等方法,分析样品及3种亚细胞组分中PAEs单体化合物的含量,进而探讨不同生育期杏果PAEs含量及其在亚细胞组分中分布的变化与二者的关联性。结果表明,从样品中共检出8种PAEs单体化合物,其中邻苯二甲酸二丁酯(DBP)单体含量最高；不同生育期杏果中的PAEs含量，不同亚细胞组分中单体化合物PAEs的含量是变化的；在不同亚细胞组分中,DBP的累积量与占比表现为可溶性组分＞细胞器＞细胞壁,与其他单体的表现不一致；随着取样时间的增加,部分试验点杏果细胞壁中邻苯二甲酸二异丁酯(DIBP)和邻苯二甲酸二甲酯(DMP)累积量和累积量占比的变化与杏果中PAEs累积量的变化相近。相关性分析结果显示,邻苯二甲酸丁基苄基酯(BBP)、DIBP、DMP、DBP含量与细胞壁组分呈显著(P<0.05)或极显著(P<0.01)正相关,相关系数分别为0.41、0.41、0.55、0.55。由研究结果得出,可溶性组分占比高可能是导致杏果中DBP含量高于其他单体的原因,细胞壁可能是影响杏果中BBP、DIBP、DMP、DBP含量变化的主要亚细胞组分。研究结果可为进一步研究PAEs在杏果内的代谢、吸收过程提供参考。
Abstract:
To clarify the changes in phthalic acid ester (PAEs) content, subcellular distribution and their correlations during different growth stages of apricots, experimental sites were established in six major apricot-producing areas in Xinjiang to collect the samples of apricots at different growth stages. A comprehensive analysis of the contents of PAEs monomer compounds in the samples and three subcellular fractions was conducted using methods such as gas chromatography-mass spectrometry and differential centrifugation. Furthermore, the changes of PAEs content and subcellular distribution during different growth stages of apricots and their correlation were investigated. The results indicated that eight PAEs monomer compounds were detected in the samples, with the highest content of the dibutyl phthalate (DBP) monomer. The content of PAEs in apricots during different growth stages and the content of PAEs monomer compounds in subcellular fractions were found to fluctuate. In different subcellular fractions, the distribution pattern of DBP showed soluble fractions > organelles > cell walls, which was inconsistent with the distribution patterns of other monomers. With the increase of sampling time, the changes in the accumulation and proportion of diisobutyl phthalate (DIBP) and dimentyl phthalate (DMP) in the cell walls of apricot fruits at some test points were similar to the changes in the accumulation of PAEs. The results of correlation analysis showed that the contents of butyl benzyl phthalate (BBP), DIBP， DMP and DBP were significantly (P<0.05) or extremely significantly (P<0.01) positively correlated with the cell wall fractions, with correlation coefficients of 0.41, 0.41, 0.55 and 0.55, respectively. Therefore, it can be seen that the high proportion of soluble fractions may be the reason why the content of DBP in apricots is higher than that of other monomers, and the cell wall may be the main subcellular fraction affecting the changes in the content of BBP, DIBP, DMP and DBP in apricots. The aforementioned results can provide a reference for further research on the metabolism and absorption processes of PAEs in apricot fruits.

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备注/Memo

备注/Memo:
收稿日期:2024-05-11基金项目:新疆维吾尔自治区重大科技专项(2022A02006-1)作者简介:李玮(2000-),女,山西交城人,硕士研究生,主要从事食品加工与安全方面的研究。(E-mail)18834288881@163.com通讯作者:王伟,(E-mail)ww2shz@163.com
更新日期/Last Update: 2025-01-20