[1]刘筠,郭毅,李效雄,等.黑果枸杞SPL转录因子家族成员鉴定与生物信息学分析[J].江苏农业学报,2024,(11):2001-2012.[doi:doi:10.3969/j.issn.1000-4440.2024.11.003]
　LIU Yun,GUO Yi,LI Xiaoxiong,et al.Identification and bioinformatics analysis of SPL transcription factor family members in Lycium ruthenicum[J].,2024,(11):2001-2012.[doi:doi:10.3969/j.issn.1000-4440.2024.11.003]
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黑果枸杞SPL转录因子家族成员鉴定与生物信息学分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年11期
页码:
2001-2012
栏目:
遗传育种·生理生化
出版日期:
2024-11-30

文章信息/Info

Title:
Identification and bioinformatics analysis of SPL transcription factor family members in Lycium ruthenicum
作者:
刘筠1; 2; 郭毅2; 李效雄3; 胡晓桐1; 2; 马瑞1; 2; 杜雨1; 2; 贾西贝1; 2; 柳迪1; 2; 马彦军1; 2
(1.甘肃农业大学林学院,甘肃兰州730070；2.甘肃林业职业技术学院,甘肃天水741020；3.兰州资源环境职业技术大学,甘肃兰州730030)
Author(s):
LIU Yun1; 2; GUO Yi2; LI Xiaoxiong3; HU Xiaotong1; 2; MA Rui1; 2; DU Yu1; 2; JIA Xibei1; 2; LIU Di1; 2; MA Yanjun1; 2
(1.College of Forestry, Gansu Agricultural University, Lanzhou 730070, China;2.Gansu Forestry Polytechnic, Tianshui 741020, China;3.Lanzhou Resources & Environment Voc-Tech University, Lanzhou 730030, China)
关键词:
黑果枸杞; NaCl胁迫; SPL转录因子; 生物信息学分析
Keywords:
Lycium ruthenicum; NaCl stress; SPL transcription factor; bioinformatics analysis
分类号:
S722.3+6
DOI:
doi:10.3969/j.issn.1000-4440.2024.11.003
文献标志码:
A
摘要:
SPL(SQUAMOSA promoter-binding protein-like)家族成员参与植物的生长发育和胁迫响应,为探究黑果枸杞SPL家族基因在盐胁迫下的耐盐分子机制,本研究对其基因家族成员进行了鉴定和生物信息学分析。结果表明,从黑果枸杞转录组数据中共筛选出20个SPL基因家族成员,分为8个亚家族,蛋白质亚细胞定位预测结果显示SPL蛋白主要定位于细胞核,20个SPL蛋白均具有SBP保守结构域。黑果枸杞SPL家族蛋白质相对分子量为15 296.08～130 310.8；等电点为5.64～9.65；总原子数为2 106～18 214；氨基酸数量为133～1 176 aa；脂溶指数为46.35～87.53；均为亲水性蛋白质和不稳定蛋白质。黑果枸杞SPL家族蛋白质结构分析结果表明,无规则卷曲和α-螺旋占比较大。表达模式分析结果表明,在NaCl胁迫下不同器官中黑果枸杞SPL家族基因具有不同的表达趋势,LrSPL9在根和叶中均为高表达。该研究结果可为黑果枸杞SPL基因家族成员盐胁迫响应方面的进一步研究提供参考。
Abstract:
The SQUAMOSA promoter-binding protein-like (SPL) family members are involved in plant growth and development and stress response. To investigate the salt tolerance molecular mechanism of Lycium ruthenicum SPL family genes under salt stress, the gene family members were identified and analyzed by bioinformatics. The results showed that 20 SPL family members were screened from transcriptome data of Lycium ruthenicum, and they were divided into eight subfamilies. The results of protein subcellular localization prediction showed that SPL proteins were mainly located in the nucleus, and 20 SPL proteins had SBP conserved domains. The relative molecular weight of SPL family proteins in Lycium ruthenicum was 15 296.08-130 310.8. The isoelectric point was 5.64-9.65, the total number of atoms was 2 106-18 214, the number of amino acids was 133-1 176 aa, and the lipid solubility index was 46.35-87.53. All of them were hydrophilic proteins and unstable proteins. The results of protein structure analysis of SPL family in Lycium ruthenicum showed that random coil and α-helix accounted for a large proportion. The results of expression pattern analysis showed that the SPL family genes of Lycium ruthenicum had different expression trends in different organs under NaCl stress, and LrSPL9 was highly expressed in roots and leaves. The results of this study can provide a reference for further research on salt stress response of SPL gene family members in Lycium ruthenicum.

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备注/Memo

备注/Memo:
收稿日期：2023-12-07 基金项目：甘肃省高校产业支撑计划项目(2023CYZC-46)；兰州资源环境职业技术大学重点研究项目(X2022ZD-05)；兰州市科技计划项目(2022-2-22) 作者简介：刘筠(1995-),女,甘肃礼县人,硕士,研究方向为林木遗传育种。(E-mail)1223318211@qq.com 通讯作者：郭毅,(E-mail)465092169@qq.com
更新日期/Last Update: 2025-01-20