[1]任恒祎,李雅慧,朱秀秀,等.甜樱桃RALF多肽家族的全基因组鉴定及表达分析[J].江苏农业学报,2024,(09):1739-1748.[doi:doi:10.3969/j.issn.1000-4440.2024.09.018]
　REN Hengyi,LI Yahui,ZHU Xiuxiu,et al.Genome-wide identification and expression analysis of the RALF polypeptide family in Prunus avium L.[J].,2024,(09):1739-1748.[doi:doi:10.3969/j.issn.1000-4440.2024.09.018]
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甜樱桃RALF多肽家族的全基因组鉴定及表达分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1739-1748
栏目:
园艺
出版日期:
2024-09-30

文章信息/Info

Title:
Genome-wide identification and expression analysis of the RALF polypeptide family in Prunus avium L.
作者:
任恒祎; 李雅慧; 朱秀秀; 许润哲; 张慧君; 李虎; 刘春; 范玉朋; 刘杰; 王继源
(淮北师范大学/安徽省西瓜甜瓜生物育种工程研究中心,安徽淮北235000)
Author(s):
REN Hengyi; LI Yahui; ZHU Xiuxiu; XU Runzhe; ZHANG Huijun; LI Hu; LIU Chun; FAN Yupeng; LIU Jie; WANG Jiyuan
(Huaibei Normal University/Anhui Provincial Watermelon and Melon Biological Breeding Engineering Research Center, Huaibei 235000, China)
关键词:
甜樱桃; 快速碱化因子（RALF）; 基因家族鉴定; 生物信息学; 表达模式分析
Keywords:
sweet cherry; rapid alkalinization factor (RALF); identification of gene family; bioinformatics; expression analysis
分类号:
S662.5
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.018
文献标志码:
A
摘要:
本研究以甜樱桃(Prunus avium L.)为试验材料,采用生物信息学分析的方法探究信号多肽快速碱化因子（RALF）家族,从甜樱桃全基因组数据库中鉴定到14个RALF相关基因,系统命名为PavRALF1～PavRALF14,从基因家族鉴定、结构分析、进化关系、保守基序和顺式作用元件等方面,对甜樱桃RALF基因进行系统鉴定分析,并分析基因在不同组织中的相对表达量。结果表明,甜樱桃RALF家族均属于疏水蛋白质,与桃(Prunus persica)RALF基因共线性关系较多,亲缘性较近,在启动子区域存在多种与植物激素和应激相关的顺式作用元件。系统进化树将RALF基因分为5个亚家族。表达特性分析结果显示,各基因成员在花、茎、叶和果实中均存在不同程度的表达。本研究结果有助于深入研究甜樱桃RALF基因家族的生物学功能以及RALF基因在甜樱桃生长发育过程中可能发挥的作用。
Abstract:
In this study, a bioinformatics analysis was conducted to investigate the rapid alkalinization factor (RALF) family in sweet cherry (Prunus avium L.). Fourteen RALF-related genes were identified from the whole genome database of sweet cherry, and were systematically named as PavRALF1-PavRALF14. The RALF of sweet cherry was systematically identified and analyzed from various aspects, including gene family identification, structural analysis, evolutionary relationships, conserved motifs, and cis-acting elements. Furthermore, the gene relative expression levels in different tissues were analyzed. The results revealed that the RALF family of sweet cherry belonged to the hydrophobic protein family and exhibited a high degree of colinearity with the RALF of Prunus persica, which showed close affinity. Numerous cis-acting elements responsive to hormone and stress response were present in the promoter region of sweet cherry RALF. RALF genes were divided into five subfamilies by phylogenetic tree. The analysis of expression characteristics showed that the gene members were differentially expressed in flowers, stems, leaves, and fruits. The results of this study will be beneficial for further exploring the biological function of the RALF gene family and its potential role in the growth and development of sweet cherry.

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备注/Memo

备注/Memo:
收稿日期:2023-12-14基金项目:安徽省高校自然科学研究重点项目(2022AH050408)作者简介:任恒祎(2001-),女,安徽宿州人,硕士研究生,主要从事植物分子育种与分子生物学研究。(E-mail)r15155532860@163.com通讯作者:王继源,(E-mail)wangjy@chnu.edu.cn
更新日期/Last Update: 2024-11-17