[1]刘珊珊,段奥其,谭杉杉,等.胡萝卜开花抑制子基因DcFLC的克隆与表达模式[J].江苏农业学报,2024,(09):1731-1738.[doi:doi:10.3969/j.issn.1000-4440.2024.09.017]
　LIU Shanshan,DUAN Aoqi,TAN Shanshan,et al.Cloning and expression profiles of flowering suppressor gene DcFLC in Daucus carota[J].,2024,(09):1731-1738.[doi:doi:10.3969/j.issn.1000-4440.2024.09.017]
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胡萝卜开花抑制子基因DcFLC的克隆与表达模式()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1731-1738
栏目:
园艺
出版日期:
2024-09-30

文章信息/Info

Title:
Cloning and expression profiles of flowering suppressor gene DcFLC in Daucus carota
作者:
刘珊珊1; 段奥其1; 谭杉杉1; 邓元杰1; 王广龙2; 刘慧1; 孙淼3; 徐志胜1; 熊爱生1
(1.南京农业大学园艺学院/作物遗传与种质创新利用全国重点实验室/农业农村部华东地区园艺作物生物学与种质创制重点实验室，江苏南京210095；2.淮阴工学院生命科学与食品工程学院，江苏淮安223003；3.盐城师范学院海洋与生物工程学院，江苏盐城224002)
Author(s):
LIU Shanshan1; DUAN Aoqi1; TAN Shanshan1; DENG Yuanjie1; WANG Guanglong2; LIU Hui1; SUN Miao3; XU Zhisheng1; XIONG Aisheng1
(1.College of Horticulture, Nanjing Agricultural University/State Key Laboratory of Crop Genetics & Germplasm Enhancement and Utilization/Key Laboratory of Biology and Germplasm Enhancement of Horticultural Crops in East China, Ministry of Agriculture and Rural Affairs， Nanjing 210095, China;2.School of Life Science and Food Engineering, Huaiyin Institute of Technology, Huai’an 223003, China;3.School of Marine and Biological Engineering, Yancheng Teachers University, Yancheng 224002, China)
关键词:
胡萝卜; 开花抑制子基因DcFLC; 表达分析; 花发育
Keywords:
carrot; flowering suppressor gene DcFLC; expression analysis; flower development
分类号:
S631.2
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.017
文献标志码:
A
摘要:
开花是植物由营养生长向生殖生长转变的重要标志。开花抑制子基因FLC是植物春化作用的关键调节基因。为了探明FLC基因在胡萝卜花发育过程中的作用，本研究以黑田五寸胡萝卜为试验材料，克隆得到胡萝卜DcFLC基因的编码序列(CDS)，并利用实时荧光定量PCR技术(RT-qPCR)分析盛花期黑田五寸胡萝卜各组织及不同发育状态花中DcFLC基因的相对表达量。结果表明，DcFLC基因的开放阅读框(ORF)长度为660 bp，编码219个氨基酸。DcFLC的相对分子量为2.454×104，理论等电点为9.15，是一个亲水性蛋白质。基于FLC编码的蛋白质氨基酸序列，胡萝卜与拟南芥、结球白菜、油菜、萝卜、杧果、白桦、可可、龙眼、葡萄、核桃等物种的遗传距离较远。盛花期胡萝卜肉质根、叶片和叶柄中DcFLC基因的表达量高于茎和花中DcFLC基因的表达量；花中开花抑制子基因DcFLC的相对表达量随着花的发育呈现先上升后下降再上升的变化趋势。本研究结果为胡萝卜FLC基因的利用和耐抽薹胡萝卜品种选育提供依据。
Abstract:
Flowering is an important symbol of the transformation of plants from vegetative growth to reproductive growth. The flowering suppressor gene FLC is a key regulator of plant vernalization. In order to explore the role of FLC gene in the development of carrot flowers, the coding sequence (CDS) of DcFLC gene was cloned from Kurodagosun carrot, and the relative expression of DcFLC gene in different tissues and flowers with different developmental states of Kurodagosun carrot at full-bloom stage was analyzed by real-time fluorescence quantitative PCR (RT-qPCR) in this study. The results showed that the open reading frame (ORF) of DcFLC gene was 660 bp in length, encoding 219 amino acids. The relative molecular weight of DcFLC was 2.454×104, and the theoretical isoelectric point was 9.15. It was a hydrophilic protein. Based on the amino acid sequence of the protein encoded by FLC, the genetic distance between carrot and Arabidopsis thaliana, Chinese cabbage, rapeseed, radish, mango, birch, cocoa, longan, grape and walnut was far. The expression of DcFLC gene in carrot fleshy roots, leaves and petioles was higher than that in stems and flowers at full-bloom stage. With the development of flowers， the relative expression of flowering suppressor gene DcFLC in flowers showed a trend of increasing first, then decreasing and then increasing. The results of this study provide a basis for the utilization of carrot FLC gene and the breeding of bolting-resistant carrot varieties.

参考文献/References:

[1]AMASINO R. Floral induction and monocarpic versus polycarpic life histories[J]. Genome Biology,2009,10(7):228.
[2]张艺能,周玉萍,陈琼华,等. 拟南芥开花时间调控的分子基础[J]. 植物学报,2014,49(4):469-482.
[3]SRIKANTH A, SCHMID M. Regulation of flowering time:all roads lead to Rome[J]. Cellular and Molecular Life Sciences,2011,68(12):2013-2037.
[4]WANG Y, LI L, YE T, et al. The inhibitory effect of ABA on floral transition is mediated by ABI5 in Arabidopsis[J]. Journal of Experimental Botany,2013,64(2):675-684.
[5]SHU K, CHEN Q, WU Y, et al. ABSCISIC ACID-INSENSITIVE 4 negatively regulates flowering through directly promoting Arabidopsis FLOWERING LOCUS C transcription[J]. Journal of Experimental Botany,2016,67(1):195-205.
[6]XIONG F, REN J J, YU Q, et al. AtU2AF65b functions in abscisic acid mediated flowering via regulating the precursor messenger RNA splicing of ABI5 and FLC in Arabidopsis[J]. New Phytologist,2019,223(1):277-292.
[7]LI Z, OU Y, ZHANG Z, et al. Brassinosteroid signaling recruits histone 3 lysine-27 demethylation activity to FLOWERING LOCUS C chromatin to inhibit the floral transition in Arabidopsis[J]. Molecular Plant,2018,11(9):1135-1146.
[8]BULGAKOV V P, AVRAMENKO T V. Linking Brassinosteroid and ABA Signaling in the Context of Stress Acclimation[J]. International Journal of Molecular Sciences,2020,21(14):5108.
[9]WENKEL S, TURCK F, SINGER K, et al. CONSTANS and the CCAAT box binding complex share a functionally important domain and interact to regulate flowering of Arabidopsis[J]. Plant Cell,2006,18(11):2971-2984.
[10]BOUVERET R, SCHONROCK N, GRUISSEM W, et al. Regulation of flowering time by Arabidopsis MSI1[J]. Development,2006,133(9):1693-1702.
[11]FINNEGAN E J, DENNIS E S. Vernal ization-induced trimethylation of histone H3 lysine 27 at FLC is not maintainedin mitotically quiescent cells[J]. Current Biology,2007,17(22):1978-1983.
[12]王莹,穆艳霞,王锦. MADS-box基因家族调控植物花器官发育研究进展[J]. 浙江农业学报,2021,33(6):1149-1158.
[13]MICHAELS S D, AMASINO R M. FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering[J]. Plant Cell,1999,11(5):949-956.
[14]LIU Y, DRENI L, ZHANG H, et al. A tea plant （Camellia sinensis） FLOWERING LOCUS C-like gene, CsFLC1,is correlated to bud dormancy and triggers early flowering in Arabidopsis[J]. International Journal of Molecular Sciences,2022,23(24):15711.
[15]TAKADA S, AKTER A, ITABASHI E, et al. The role of FRIGIDA and FLOWERING LOCUS C genes in flowering time of Brassica rapa leafy vegetables[J]. Scientific Reports,2019,9:13843.
[16]庄飞云,欧承刚,赵志伟. 胡萝卜育种回顾及展望[J]. 中国蔬菜,2008(3):41-44.
[17]鲍生有,欧承刚,庄飞云,等. 胡萝卜春季栽培先期抽薹的调查与分析[J]. 中国蔬菜,2010(6):38-42.
[18]张榕蓉,王雅慧,李彤,等. 胡萝卜WRKY69基因(DcWRKY69)的克隆及其对不同植物生长调节剂的响应[J]. 植物资源与环境学报,2019,28(4):1-10.
[19]XU Z S, TAN H W, WANG F, et al. CarrotDB: a genomic and transcriptomic database for carrot[J]. Database,2014,2014：bau096.
[20]KUMAR S, STECHER G, TAMURA K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular Biology and Evolution,2016,33(7):1870-1874.
[21]刘文艳,刘春方,滕瑞敏,等. 茶树生物钟基因CsCCA1的鉴定及对光合特性的影响[J]. 植物生理学报,2022,58(3):554-564.
[22]TIAN C, JIANG Q, WANG F, et al. Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves[J]. PLoS One,2015,10(2):e0117569.
[23]SCHAFFER A A, ARAVIND L, MADDEN T L, et al. Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements[J]. Nucleic Acids Research,2001,29(14): 2994-3005.
[24]ALVAREZ-BUYLLA E R, PELAZ S, LILJEGREN S J, et al. An ancestral MADS-box gene duplication occurred before the divergence of plants and animals[J]. Proceedings of the National Academy of Sciences of the United States of America,2000,97(10):5328-5333.
[25]TADEGE M, SHELDON C C, HELLIWELL C A, et al. Control of flowering time by FLC orthologues in Brassica napus[J]. Plant Journal,2001,28(5):545-553.
[26]OKAZAKI K, SAKAMOTO K, KIKUCHI R, et al. Mapping and characterization of FLC homologs and QTL analysis of flowering time in Brassica oleracea[J]. Theoretical and Applied Genetics,2007,114(4):595-608.
[27]MATEOS J L, MADRIGAL P, TSUDA K, et al. Combinatorial activities of SHORT VEGETATIVE PHASE and FLOWERING LOCUS C define distinct modes of flowering regulation in Arabidopsis[J]. Genome Biology,2015,16:31.
[28]MICHAELS S D, AMASINO R M. Memories of winter: vernalization and the competence to flower[J]. Plant Cell and Environment,2000,23(11):1145-1153.
[29]荣子龙,侯喜林,史公军,等. 不结球白菜晚抽薹BcFLC1基因克隆及表达分析[J]. 南京农业大学学报,2010,33(6):23-27.
[30]展妍丽,王萃铂,亓钰莹,等. 菊花开花抑制基因CmFLC-like1的克隆及表达特性分析[J]. 园艺学报,2015,42(7):1347-1355.
[31]祁广俊,刘德兵,魏军亚,等. 芒果FLC同源基因的克隆和表达分析[J]. 分子植物育种,2020,18(18):5951-5957.
[32]NAH G, CHEN Z J. Tandem duplication of the FLC locus and the origin of a new gene in Arabidopsis related species and their functional implications in allopolyploids[J]. New Phytologist,2010,186(1):228-238.
[33]袁敏,邢朝斌,葛伟娜,等. 拟南芥开花诱导基因FT的蛋白表达及纯化[J]. 基因组学与应用生物学,2017,36(8):3053-3056.
[34]HE Y H. Control of the transition to flowering by chromatin modifications[J]. Molecular Plant,2009,2(4):554-564.
[35]徐雷,贾飞飞,王利琳. 拟南芥开花诱导途径分子机制研究进展[J]. 西北植物学报,2011,31(5):1057-1065.
[36]FAN M Y, CHEN L F, WANG E R, et al. Identification and characterization of flowering time regulatory gene FLC of Paeonia ostii ‘Fengdan’[J]. Scientia Horticulturae,2023,310:111748.

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备注/Memo

备注/Memo:
收稿日期：2023-09-26基金项目：国家自然科学基金项目(32072563、32102369)；江苏省自然科学基金项目(BK20211366)；江苏高校优势学科建设项目(PAPD)作者简介：刘珊珊(2000-)，女，安徽淮北人，硕士研究生，主要从事蔬菜遗传育种研究。(E-mail)2022104058@stu.njau.edu.cn通讯作者：熊爱生，(E-mail) xiongaisheng@njau.edu.cn
更新日期/Last Update: 2024-11-17