[1]谢波艳,孔丽静,赵文君,等.基于形态性状和SSR标记的140份北苍术种质多样性分析[J].江苏农业学报,2024,(09):1607-1616.[doi:doi:10.3969/j.issn.1000-4440.2024.09.004]
　XIE Boyan,KONG Lijing,ZHAO Wenjun,et al.Diversity analysis of 140 Atractylodes chinensis （DC.） Koidz. germplasms based on morphological traits and SSR markers[J].,2024,(09):1607-1616.[doi:doi:10.3969/j.issn.1000-4440.2024.09.004]
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基于形态性状和SSR标记的140份北苍术种质多样性分析()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年09期
页码:
1607-1616
栏目:
遗传育种·生理生化
出版日期:
2024-09-30

文章信息/Info

Title:
Diversity analysis of 140 Atractylodes chinensis （DC.） Koidz. germplasms based on morphological traits and SSR markers
作者:
谢波艳1; 孔丽静1; 赵文君1; 苏书乐1; 张赛1; 张煜彬1; 孙成龙1; 赵思源1; 张明慧1
(1.河北科技师范学院农学与生物科技学院/河北省作物逆境生物学重点实验室,河北秦皇岛066004；2.河北大学中医学院,河北保定071200；3.南京农业大学园艺学院,江苏南京210095)
Author(s):
XIE Boyan1; KONG Lijing1; ZHAO Wenjun1; SU Shuyue1; ZHANG Sai1; ZHANG Yubin1; SUN Chenglong1; ZHAO Siyuan1; ZHANG Minghui1
(1.College of Agronomy and Biotechnology, Hebei Normal University of Science and Technology/Hebei Provincial Key Laboratory of Crop Stress Biology, Qinhuangdao 066004, China；2.College of Traditional Chinese Medicine, Hebei University, Baoding 071200, China；3.College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China)
关键词:
北苍术; 形态指标; 分子标记; 主成分分析; 聚类分析; 种质多样性
Keywords:
Atractylodes chinensis (DC.) Koidz.; morphological indicators; molecular markers; principal component analysis; clustering analysis; germplasm diversity
分类号:
S567.21+1
DOI:
doi:10.3969/j.issn.1000-4440.2024.09.004
文献标志码:
A
摘要:
本研究以140份北苍术种质为材料,基于13个形态指标进行形态多样性分析,并利用SSR分子标记技术对其进行遗传多样性分析。结果表明,13个形态指标变异系数为1.93%～63.11%,其中主茎粗变异系数最大,花展开度的Shannon-Weiner多样性指数最大。主成分分析结果表明,种子长度、花展开度、主茎粗、株高、主茎与分枝夹角是反映北苍术形态多样性的主要指标。基于13个形态指标可以将140份北苍术种质聚为4个类群。利用筛选出的8对SSR引物扩增北苍术基因组DNA,根据特异性条带将140份北苍术种质聚为3个类群。13个形态指标和3个遗传多样性指标间相关性分析结果表明,单花结籽数与多态性信息量指数（PIC）呈显著正相关(P＜0.05)；主茎粗与Nei’s基因多样性指数(h)呈显著负相关(P＜0.05)。综上,140份北苍术种质在形态指标和遗传多样性指标上均具有丰富的多样性,且形态指标与遗传多样性指标间具有一定的相关性。本研究为北苍术种质资源鉴定与利用提供了理论依据。
Abstract:
In this study, 140 Atractylodes chinensis (DC.) Koidz. germplasms were used as the test materials. The morphological diversity of 140 Atractylodes chinensis (DC.) Koidz. germplasms was analyzed based on 13 morphological indicators, and the genetic diversity was analyzed by SSR molecular markers. The results showed that the variation coefficients of 13 morphological indicators were 1.93%-63.11%. The variation coefficient of main stem diameter was the largest, and the Shannon-Weiner diversity index of flower spread was the largest. The results of principal component analysis showed that seed length, flower spread, main stem diameter, plant height and angle between main stem and branches were the main indicators reflecting the morphological diversity of Atractylodes chinensis (DC.) Koidz. Based on 13 morphological indicators, 140 Atractylodes chinensis (DC.) Koidz. germplasms could be clustered into four groups. The genomic DNA of Atractylodes chinensis (DC.) Koidz. was amplified with eight pairs of SSR primers, and 140 Atractylodes chinensis (DC.) Koidz. germplasms were clustered into three groups according to the specific bands. The results of correlation analysis between 13 morphological indicators and three genetic diversity indicators showed that seed number per flower was significantly positive correlated with polymorphic information content （PIC）(P＜0.05). There was a significantly negative correlation between main stem diameter and Nei’s gene diversity index (h)(P＜0.05). In conclusion, 140 Atractylodes chinensis (DC.) Koidz. germplasms had abundant diversity in morphological and genetic diversity indicators, and there was a certain correlation between morphological and genetic diversity indicators. This study provides a theoretical basis for the identification and utilization of Atractylodes chinensis (DC.) Koidz. germplasm resources.

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备注/Memo

备注/Memo:
收稿日期：2023-12-21基金项目：河北省高等学校科学研究项目(ZD2022014)；承德市科技计划项目(202304B054)作者简介：谢波艳(1998-),女,江苏南通人,硕士研究生,研究方向为药用种质资源分析。(E-mail)1797491496@qq.com通讯作者：郑金双,(E-mail)jinshuangk@163.com
更新日期/Last Update: 2024-11-17