[1]邹宜芯,李慧,任玉欣,等.硫代葡萄糖苷浸种对青花菜种子萌发及生理特性的影响[J].江苏农业学报,2024,(08):1379-1388.[doi:doi:10.3969/j.issn.1000-4440.2024.08.003]
　ZOU Yixin,LI Hui,REN Yuxin,et al.Effects of soaking seeds with glucosinolates on seed germination and physiological characteristics of broccoli[J].,2024,(08):1379-1388.[doi:doi:10.3969/j.issn.1000-4440.2024.08.003]
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硫代葡萄糖苷浸种对青花菜种子萌发及生理特性的影响()
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江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年08期
页码:
1379-1388
栏目:
遗传育种·生理生化
出版日期:
2024-08-30

文章信息/Info

Title:
Effects of soaking seeds with glucosinolates on seed germination and physiological characteristics of broccoli
作者:
邹宜芯; 李慧; 任玉欣; 赵凯文
(天津农学院园艺园林学院,天津300392)
Author(s):
ZOU Yixin; LI Hui; REN Yuxin; ZHAO Kaiwen
(College of Horticulture and Landscape Architecture, Tianjin Agricultural University, Tianjin 300392, China)
关键词:
青花菜; 硫代葡萄糖苷; 种子萌发; 生理特性; 抗逆性
Keywords:
broccoli; glucosinolates; seed germination; physiological characteristics; stress resistance
分类号:
S635.3
DOI:
doi:10.3969/j.issn.1000-4440.2024.08.003
文献标志码:
A
摘要:
以青花菜种子为试验材料,采用培养皿进行发芽试验,设置蒸馏水、200 mmol/L NaCl和15% PEG-6000 3种培养条件,研究不同质量浓度[0 μg/mL(CK)、0.94 μg/mL、1.88 μg/mL、3.75 μg/mL、7.50 μg/mL、15.00 μg/mL]硫代葡萄糖苷浸种对青花菜种子萌发指标、幼苗形态指标及生理指标的影响。研究结果表明,与CK相比,蒸馏水培养下,硫代葡萄糖苷浸种对青花菜种子的发芽率和发芽势没有显著影响，7.50 μg/mL硫代葡萄糖苷浸种的青花菜根冠比显著提高；1.88～7.50 μg/mL硫代葡萄糖苷浸种的青花菜POD活性显著高于对照,其中3.75 μg/mL硫代葡萄糖苷浸种能显著提高青花菜的SOD和CAT活性,且MDA含量显著低于对照。盐胁迫下,7.50 μg/mL硫代葡萄糖苷浸种能显著提高青花菜的SOD、POD活性,显著降低MDA含量。干旱胁迫下,硫代葡萄糖苷浸种均能显著提高青花菜种子萌发指标,显著降低青花菜MDA含量；0.94 μg/mL硫代葡萄糖苷浸种能显著增加青花菜的主根长,1.88 μg/mL和3.75 μg/mL硫代葡萄糖苷浸种能显著提高青花菜的根冠比；15.00 μg/mL硫代葡萄糖苷浸种能显著增加青花菜的POD活性。隶属函数分析结果表明,蒸馏水培养和干旱胁迫下3.75 μg/mL硫代葡萄糖苷浸种及盐胁迫下7.50 μg/mL硫代葡萄糖苷浸种的隶属函数值最高。综上所述,硫代葡萄糖苷浸种能有效缓解青花菜种子萌发期和幼苗期旱害和盐害,干旱胁迫下浸种最适质量浓度为3.75 μg/mL，盐胁迫下浸种最适质量浓度为7.50 μg/mL。
Abstract:
In this study, broccoli seeds were used as experimental materials, and germination experiments were carried out using culture dishes. Three culture conditions of distilled water, 200 mmol/L NaCl and 15% PEG-6000 were set up. Six different concentrations of glucosinolates were set as 0 μg/mL (CK), 0.94 μg/mL, 1.88 μg/mL, 3.75 μg/mL, 7.50 μg/mL and 15.00 μg/mL. The effects of different mass concentrations of glucosinolates on seed germination indices, seedling morphological indices and physiological indices of broccoli were studied. The results showed that compared with CK, soaking seeds with glucosinolates had no significant effect on the germination rate and germination potential of broccoli under distilled water culture, but the root-shoot ratio of broccoli in the treatment of soaking seeds with 7.50 μg/mL glucosinolates was significantly increased. The POD activity of broccoli in the treatment of soaking seeds with 1.88-7.50 μg/mL glucosinolates was significantly higher than that in the control, and the SOD and CAT activities of broccoli in the treatment of soaking seeds with 3.75 μg/mL glucosinolates were significantly increased, and the MDA content was significantly lower than that in the control. Under salt stress, soaking seeds with 7.50 μg/mL glucosinolates could significantly increase the activities of SOD and POD, and significantly reduce the content of MDA in broccoli. Under drought stress, soaking seeds with glucosinolates could significantly improve the germination indices of broccoli seeds and significantly reduce the MDA content of broccoli. Soaking seeds with 0.94 μg/mL glucosinolates could significantly increase the main root length of broccoli, and soaking seeds with 1.88 μg/mL and 3.75 μg/mL glucosinolates could significantly increase the root-shoot ratio of broccoli. Soaking seeds with 15.00 μg/mL glucosinolates could significantly increase the POD activity of broccoli. The results of membership function analysis showed that the membership function values of 3.75 μg/mL glucosinolate soaking treatment under distilled water culture and drought stress and 7.50 μg/mL glucosinolate soaking treatment under salt stress were the highest. In summary, soaking seeds with glucosinolates can effectively alleviate the drought and salt damage of broccoli during seed germination and seedling stage， the optimum concentration of glucosinolates for soaking seeds under drought stress is 3.75 μg/mL, and the optimum concentration of glucosinolates for soaking seeds under salt stress is 7.50 μg/mL.

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备注/Memo

备注/Memo:
收稿日期:2023-10-16基金项目:天津市科技计划种业科技重大专项(18ZXZYNC00160)；天津市大学生创新训练计划项目(202210061104)作者简介:邹宜芯(1999-),女,四川成都人,硕士研究生,主要从事蔬菜栽培生理研究。(E-mail)913788290@qq.com通讯作者:李慧,(E-mail)lihui@tjau.edu.cn
更新日期/Last Update: 2024-09-18