[1]吕梦炀,马亚特,肖丽静,等.尾梢去除处理对板栗结果枝基部芽激素含量的影响[J].江苏农业学报,2024,(05):922-934.[doi:doi:10.3969/j.issn.1000-4440.2024.05.017]
　LYU Mengyang,MA Yate,XIAO Lijing,et al.Effects of tail tip removal treatments on the content of hormones in the basal buds of chestnut fruiting branches[J].,2024,(05):922-934.[doi:doi:10.3969/j.issn.1000-4440.2024.05.017]
点击复制

尾梢去除处理对板栗结果枝基部芽激素含量的影响()
分享到：

江苏农业学报[ISSN:1006-6977/CN:61-1281/TN]

卷:
期数:
2024年05期
页码:
922-934
栏目:
园艺
出版日期:
2024-05-30

文章信息/Info

Title:
Effects of tail tip removal treatments on the content of hormones in the basal buds of chestnut fruiting branches
作者:
吕梦炀1; 2; 3; 马亚特1; 2; 3; 肖丽静1; 2; 3; 张海娥2; 4; 齐永顺1; 2; 3; 4; 张京政2; 4; 王东升2; 4; 5
(1.河北科技师范学院园艺科技学院,河北秦皇岛066004；2.河北科技师范学院板栗产业技术教育部工程研究中心,河北秦皇岛066004；3.河北省特色园艺种质挖掘与创新利用重点实验室,河北秦皇岛066004；4.河北省板栗产业协同创新中心,河北秦皇岛066004；5.河北省燕山特色农业产业技术研究院,河北秦皇岛066004)
Author(s):
LYU Mengyang1; 2; 3; MA Yate1; 2; 3; XIAO Lijing1; 2; 3; ZHANG Hai’e2; 4; QI Yongshun1; 2; 3; 4; ZHANG Jingzheng2; 4; WANG Dongsheng2; 4; 5
(1.College of Horticultural Science and Technology, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;2.Engineering Research Center of Chestnut Industry Technology, Ministry of Education, Hebei Normal University of Science and Technology, Qinhuangdao 066004, China;3.Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Qinhuangdao 066004, China;4.Hebei Collaborative Innovation Center of Chestnut Industry, Qinhuangdao 066004, China;5.Hebei Yanshan Characteristic Agricultural Technology Research Institute, Qinhuangdao 066004, China)
关键词:
板栗; 尾梢处理; 基部芽; 液相色谱串联质谱技术; 激素
Keywords:
chestnut; tail tip treatment; basal bud; liquid chromatography tandem mass spectrometry; hormone
分类号:
S664.2
DOI:
doi:10.3969/j.issn.1000-4440.2024.05.017
摘要:
分析尾梢去除处理对基部芽激素含量的影响,可为了解隐芽萌发条件提供一定的参考。本研究以燕秋为试验材料,分别在花后第1 d(TA,5月25日)、第21 d(TB,6月14日)和第41 d(TC,7月4日)进行尾梢去除处理,并在每次去除尾梢后第5 d、第10 d、第15 d、第20 d取1次基部芽。采用液相色谱串联质谱(LC-MS/MS)技术测定不同处理的板栗结果枝基部芽中的独脚金内酯(SL)含量、赤霉素(GA)含量、生长素(Auxin)含量、细胞分裂素(CTK)含量、脱落酸(ABA)含量及其比值变化。结果表明,对板栗结果枝去除尾梢后均促进了基部芽平均萌发一年生枝条总数；TA 处理提高了基部芽平均萌发一年生结果枝占比(16.67%)、一年生枝平均发育枝枝长及结果枝枝粗；TB处理、TC处理后基部芽只萌发出了发育枝。对照基部芽未萌发,仅顶端芽萌发一年生枝,平均为3.0个,其中结果枝占比为20.00%。尾梢去除后SL含量在TA处理下降幅度较大；GA含量在TB处理下降幅度较大；Auxin含量在TA处理下降,TB处理上升幅度较大；CTK含量波动幅度与对照相似,差异不显著；ABA含量在TC处理上升幅度较大,而在TA处理下降。吲哚-3-乙酸（IAA）含量与ABA含量的比值总体下降,其中TC处理下降幅度较大；IAA含量与GA含量的比值在TC处理上升幅度较大,波动较明显；ABA含量与GA含量的比值在TA处理下降，TC处理上升幅度较大。此外,相关性分析结果显示,对照基部芽赤霉素含量和脱落酸含量呈显著正相关(r=0.646),而处理基部芽29种激素间相关性主要集中在细胞分裂素和生长素之间,其中IA(吲哚-3-丙烯酸)含量与IAA-Ala[N-(3-吲哚乙酰基)-L-丙氨酸]、ICA(吲哚-3-甲酸)、TRA(色胺)、cZROG(顺式-玉米核苷-O-糖苷)、ABA-GE(脱落酸葡萄糖酯)、DHZ7G(双氢玉米素-7-糖苷)、ICAld(吲哚-3-甲醛)、tZOG(反式-玉米素-9-B-葡萄糖苷)的含量呈极显著正相关；IPR(异戊烯腺嘌呤核苷)含量、tZR(玉米素核苷)含量均与TRA含量、ILA(吲哚-3-乳酸)含量、cZROG(顺式-玉米核苷-O-糖苷)含量呈极显著负相关。综合考虑，对板栗进行尾梢去除后会促进基部芽的萌发,同时TA处理会促使基部腋芽萌发结果枝。尾梢去除后整体上降低了GA的含量,低水平的GA含量有助于促进腋芽生长。低水平的ABA含量有助于基部芽萌发结果枝；高水平ABA含量与GA含量的比值会抑制腋芽萌发结果枝。合理区间内的低水平IAA含量,可能会促进板栗基部芽萌发结果枝。
Abstract:
Analyzing the effects of tail tip removal treatments on the content of hormones in basal buds can provide a certain reference for understanding the germination conditions of hidden buds. In this study, Yanqiu was used as the experimental material. Tail tip removal treatments were performed on the first day (TA, May 25), 21st day (TB, June 14) and 41st day (TC, July 4) after flowering. The basal buds were taken on the 5th, 10th, 15th, and 20th days after each removal of the tail tip. The contents and ratios of strigolactone (SL), gibberellin (GA), auxin, cytokinin (CTK) and abscisic acid (ABA) in the basal buds of chestnut fruiting branches after different treatments were determined by liquid chromatography tandem mass spectrometry (LC-MS/MS). The results showed that the average number of one-year-old branches germinated from the basal buds was promoted after the removal of tail tip of chestnut fruiting branches. TA treatment increased the ratio of fruiting branches (16.67%), branch length of developing branches and the diameter of fruiting branches. After TB treatment and TC treatment, only developing branches germinated from the basal buds. The basal buds of the control did not germinate, and only the top buds germinated one-year-old branches, with an average of three branches, and the ratio of fruiting branches was 20.00%. The SL content decreased greatly in TA treatment. The content of GA decreased greatly in TB treatment. The content of auxin decreased in TA treatment, and increased greatly in TB treatment. The fluctuation range of CTK content was similar to that of the control, and the difference was not significant. The ABA content increased greatly in TC treatment, but decreased in TA treatment. The ratio of indole-3-acetic acid (IAA) content to ABA content decreased overall, and it decreased greatly in TC treatment. The ratio of IAA content to GA content increased greatly in TC treatment, and the fluctuation was obvious. The ratio of ABA content to GA content decreased in TA treatment, and increased significantly in TC treatment. In addition, correlation analysis showed that there was a significant positive correlation between gibberellin content and abscisic acid content in the control (r=0.646). The correlation between 29 hormones in the treatments was mainly concentrated between cytokinin and auxin. Among them, the content of IA (3-indoleacrylic acid) was significantly positively correlated with the contents of IAA-Ala [N-(3-indolylacetyl)-L-alanine], ICA (indole-3-carboxylic acid), TRA (tryptamine), cZROG (cis-zeatin O-glucoside riboside), ABA-GE (abscisic acid-glucosyl ester), DHZ7G (dihydrozeatin-7-glucoside), ICAld (indole-3-carboxaldehyde), tZOG (trans-zeatin-O-glucoside). The IPR (N6-isopentenyladenosine) content and tZR (trans-zeatin riboside) content were significantly negatively correlated with TRA content, ILA (indole-3-lactic acid) content and cZROG (cis-zeatin-O-glucoside riboside) content. In summary, the removal of tail tip of the chestnut could promote the germination of the basal buds, and the TA treatment could promote the germination of the fruiting branches from the basal axillary buds. After the removal of the tail tip, the content of GA decreased as a whole, and the low level of GA was helpful to promote the growth of axillary buds. The low level of ABA content was helpful for the germination of fruiting branches from basal buds. High levels of the ratio of ABA content to GA content could inhibit the germination of fruiting branches from axillary buds. The low level of IAA content in a reasonable range may promote the germination of chestnut fruiting branches from basal buds.

参考文献/References:

[1]李勇革. 我国板栗主栽品种及丰产栽培技术[J]. 四川果树,1997(4):34-35.
[2]徐志祥,高绘菊. 板栗营养价值及其养生保健功能[J]. 食品研究与开发,2004(5):118-119.
[3]王金叶. 板栗丰产园修剪技术[J]. 农民致富之友,2015(2):134.
[4]韩元顺,许林云,周杰. 中国板栗产业与市场发展现状及趋势[J]. 中国果树,2021(4):83-88.
[5]吴大瑜,江锡兵,龚榜初,等. 浙江省板栗产业发展现状及建议[J]. 中国果树,2019(1):103-106,109.
[6]张树航,王广鹏,李颖,等. 板栗轮替更新整形修剪技术中打“轮替橛”的三个关键要点[J]. 河北果树,2018(5):55.
[7]高春国. 板栗轮替更新修剪技术[J]. 北方果树,2022(2):41-42.
[8]李慧峰,王海波,李林光,等. 苹果树摘心效应[J]. 落叶果树,2011,43(2):11-12.
[9]孔繁星. 摘心打顶技术在密植幼龄茶园中的示范应用效果[J]. 南方农业,2022,16(17):110-112,116.
[10]刘拥海,俞乐,丁君辉,等. 植物激素对分枝发育的协同调控作用研究进展[J]. 植物生理学报,2012,48(10):941-948.
[11]查三省. 板栗雌花序分化内源激素及分子调控机理研究[D]. 武汉:武汉轻工大学,2019.
[12] MLLER D, LEYSER O. Auxin, cytokinin and the control of shoot branching[J]. Annals of Botany,2011,107(7):1203-1212.
[13] WANG Q, KOHLEN W, ROSSMANN S, et al. Auxin depletion from the leaf axil conditions competence for axillary meristem formation in Arabidopsis and tomato[J]. Plant Cell,2014,26(5):2068-2079.
[14] NI J, GAO C, CHEN M S, et al. Gibberellin promotes shoot branching in the perennial woody plant Jatropha curcas[J]. Plant and Cell Physiology,2015,8(56):1655-1666.
[15] YAO C, FINLAYSON S A. Abscisic acid is a general negative regulator of Arabidopsis axillary bud growth[J]. Plant Physiology,2015,169(1):611-626.
[16] GOMEZ-ROLDAN V, FERMAS S, BREWER P B, et al. Strigolactone inhibition of shoot branching[J]. Nature,2008,455(7210):189-194.
[17] TANTIKANJANA T, YONG J W, LETHAM D S, et al. Control of axillary bud initiation and shoot architecture in Arabidopsis through the supershoot gene[J]. Genes Dev,2001,15(12):1577-1588.
[18]程华,李琳玲,袁红慧,等. 内源激素含量变化与板栗花芽分化关系研究[J]. 北方园艺,2013(22):5-9.
[19]李从玉,陈在新. 板栗雄花芽临界分化期内源激素含量变化[J]. 安徽农业科学,2012,40(2):680-681,684.
[20]郭成圆,魏安智,吕平会,等. 板栗雏梢分化期内源激素的动态变化特征[J]. 西北植物学报,2010,30(10):2061-2066.
[21] JANSSEN J B, DRUMMOND S R, SNOWDEN C K. Regulation of axillary shoot development [J]. Current Opinion in Plant Biology,2014,17:28-35.
[22]蒋彭炎,洪晓富,冯来定,等. 从水稻个体间同化物的分配动态论分蘖利用[J]. 浙江农业学报,1994,6(4):209-213.
[23]苏强,陈闯,曹飞,等. 板栗花芽分化生理及分子机理研究进展[J]. 河北果树,2023(2):1-3,12.
[24]欧阳芬. 锥栗花芽分化特性研究[D]. 长沙: 中南林业科技大学,2017.
[25]白志英,路丙社,张林平,等. 板栗雄花分化研究[J]. 经济林研究,2000(3):11-12,44.
[26]苏强. 三个不同类型板栗雌花花芽分化的观察及生理基础[D]. 秦皇岛:河北科技师范学院,2023.
[27]王璐. 板栗混合花序分化生理特征及其转录组分析[D]. 长沙:中南林业科技大学,2023.
[28]范吴蔚,潘志演,王原秀,等. 独脚金内酯对烟草腋芽生长以及抗氧化酶活性和糖含量的影响[J]. 植物生理学报,2021,57(4):910-918.
[29]王闵霞,彭 鹏,龙海馨,等. 独脚金内酯途径相关基因的研究进展[J]. 分子植物育种,2014,12(3):603-609.
[30]曾芳,高娅,潘鑫,等. 调控穗发芽的植物内源激素研究进展[J]. 江苏农业学报,2023,39(3):848-858.
[31]范晨伟,田丽波,商桑,等. 黄瓜果实内源激素与果实空腔的关系[J]. 南方农业学报,2022,53(11):3174-3183.
[32] ANTONIO S, STEFANO B, MAX B, et al. DELLA genes restrict inflorescence meristem function independently of plant height[J]. Nat Plants,2017,3(9):749-754.
[33] LUISI A, LORENZI R, SORCE C. Strigolactone may interact with gibberellin to control apical dominance in pea (Pisum sativum)[J]. Plant Growth Regulation,2011,65(2):415-419.
[34]杨成龙,方少忠,黄永旺,等. 外源生长素影响百合地下茎生鳞茎发生的转录组分析[J]. 南方农业学报,2023,54(11):3156-3164.
[35]黄双杰,曹梦珍,陈凌芝,等. 氮素胁迫条件下茶树根系发育及生长素的响应[J]. 江苏农业学报,2023,39(3):814-821.
[36]廖焕琴,杨会肖,徐放,等. 尾叶桉不同无性系组培生根相关基因的表达分析[J]. 南京林业大学学报(自然科学版),2023,47(4):114-122,265-276.
[37]何宏涛,王玉虎,周洪友,等. 番茄根际产生长素菌株分离及其对番茄和马铃薯幼苗的促生作用[J]. 江苏农业科学,2022,50(19):219-225.
[38]张焕玲. 金叶复叶槭水培嫩枝组织培养体系研究[J]. 南京林业大学学报(自然科学版),2022,46(5):89-94.
[39] LEYSER O. The fall and rise of apical dominance[J]. Current Opinion in Genetics & Development,2005,15(4):468-471.
[40] 王原秀,唐博希,田慧源,等. 脱落酸对植物芽休眠和侧枝生长的调控[J/OL]. 分子植物育种,2023:1-13. https://kns.cnki.net/kcms2/detail/46.1068.S.20230706.1944.014.html.
[41] DONG Z B, XIAO Y G, RAJANIKANTH G, et al. The regulatory landscape of a core maize domestication module controlling bud dormancy and growth repression[J]. Nature Communications, 2019,10(1):3810.
[42] GEUNS J M C, SMETS R, STRUYF T, et al. Apical dominance in Pssu-ipt-transformed tobacco[J]. Phytochemistry,2001,58(6):911-921.
[43] CLINE M G, OH C. A reappraisal of the role of abscisic acid and its interaction with auxin in apical dominance[J]. Ann Bot,2006,98(4):891-897.
[44]禹利君,史云峰,肖海云,等. 不同物候型茶树内源GA3和ABA的变化及其对腋芽萌发调控的影响[J]. 作物学报,2008,34(2):277-283.
[45]朱思雅,李雪娇,赵雁,等. 去顶诱导的食用玫瑰‘滇红’腋芽萌发及内源激素含量变化[J/OL]. 分子植物育种,2022:1-12. https://kns.cnki.net/kcms/detail/46.1068.S.20220804.1416.006.html.

相似文献/References:

[1]王大红,张帅滢,宋鹏辉,等.纳他霉素复合涂膜剂对板栗的保鲜效果[J].江苏农业学报,2018,(03):679.[doi:doi:10.3969/j.issn.1000-4440.2018.03.029]
　WANG Da-hong,ZHANG Shuai-ying,SONG Peng-hui,et al.Effects of natamycin coating compounds on preservation of chestnut during storage[J].,2018,(05):679.[doi:doi:10.3969/j.issn.1000-4440.2018.03.029]

备注/Memo

备注/Memo:
收稿日期：2024-01-30基金项目：国家重点研发计划项目(2022YFD2200400)作者简介：吕梦炀(1998-),女,河北秦皇岛人,硕士研究生,研究方向为果树栽培育种。(Tel)18232394008;(E-mail)892415636@qq.com通讯作者：王东升,(Tel)13603235351;(E-mail)wdsgfly@126.com
更新日期/Last Update: 2024-07-13